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Assessment of a 27-kDa antigen in Enzyme-Linked Immunosorbent Assay for the diagnosis of fasciolosis in Vietnamese patients

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Abstract
Fasciolosis has emerged as an important zoonotic disease in many parts of the world. In recent years, an increasing number of human cases were reported in Vietnam. In this study, the 27-kDa component protein from the excretory/secretory production of adult Fasciola gigantica, purified by high performance liquid chromatography, was assessed in an enzyme-linked immunosorbent assay (ELISA) to detect antibodies against Fasciola spp. for diagnosis of human fasciolosis. The ELISA showed a high sensitivity (100%) and specificity (97.67%) when tested on patients with fasciolosis, other parasitic infections, cholangiocarcinoma and on healthy controls. The assay was applied for diagnosis on 143 patients in the Viet Duc-Hanoi hospital who presented with clinical signs of liver disease and lesions in their livers as shown by imaging techniques. Antibodies were found in 37 (25.9%) of these patients, of whom only 3 shed Fasciola eggs in their stools (2.1%). The excellent response to triclabendazole treatment of 37 sero-positive patients confirmed the diagnosis of fasciolosis. This study demonstrated the diagnostic potential for human fasciolosis of the 27-kDa antigen ELISA. Fasciolosis should be considered in the differential diagnosis of hepatic disease in Vietnam.
Keywords
diagnosis, Fasciolosis, Enzyme-linked immunosorbent assay, hepatic disease, Vietnam, HEPATICA, GIGANTICA, SERODIAGNOSIS, CHOLANGIOCARCINOMA, TRICLABENDAZOLE, SHEEP

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MLA
Nguyen Thi, Giang Thanh, TH Le, NV De, et al. “Assessment of a 27-kDa Antigen in Enzyme-Linked Immunosorbent Assay for the Diagnosis of Fasciolosis in Vietnamese Patients.” TROPICAL MEDICINE & INTERNATIONAL HEALTH 15.4 (2010): 462–467. Print.
APA
Nguyen Thi, G. T., Le, T., De, N., Doan, T., Dao, T., Vercruysse, J., & Dorny, P. (2010). Assessment of a 27-kDa antigen in Enzyme-Linked Immunosorbent Assay for the diagnosis of fasciolosis in Vietnamese patients. TROPICAL MEDICINE & INTERNATIONAL HEALTH, 15(4), 462–467.
Chicago author-date
Nguyen Thi, Giang Thanh, TH Le, NV De, TT Doan, THT Dao, Jozef Vercruysse, and Pierre Dorny. 2010. “Assessment of a 27-kDa Antigen in Enzyme-Linked Immunosorbent Assay for the Diagnosis of Fasciolosis in Vietnamese Patients.” Tropical Medicine & International Health 15 (4): 462–467.
Chicago author-date (all authors)
Nguyen Thi, Giang Thanh, TH Le, NV De, TT Doan, THT Dao, Jozef Vercruysse, and Pierre Dorny. 2010. “Assessment of a 27-kDa Antigen in Enzyme-Linked Immunosorbent Assay for the Diagnosis of Fasciolosis in Vietnamese Patients.” Tropical Medicine & International Health 15 (4): 462–467.
Vancouver
1.
Nguyen Thi GT, Le T, De N, Doan T, Dao T, Vercruysse J, et al. Assessment of a 27-kDa antigen in Enzyme-Linked Immunosorbent Assay for the diagnosis of fasciolosis in Vietnamese patients. TROPICAL MEDICINE & INTERNATIONAL HEALTH. 2010;15(4):462–7.
IEEE
[1]
G. T. Nguyen Thi et al., “Assessment of a 27-kDa antigen in Enzyme-Linked Immunosorbent Assay for the diagnosis of fasciolosis in Vietnamese patients,” TROPICAL MEDICINE & INTERNATIONAL HEALTH, vol. 15, no. 4, pp. 462–467, 2010.
@article{886204,
  abstract     = {Fasciolosis has emerged as an important zoonotic disease in many parts of the world. In recent years, an increasing number of human cases were reported in Vietnam. In this study, the 27-kDa component protein from the excretory/secretory production of adult Fasciola gigantica, purified by high performance liquid chromatography, was assessed in an enzyme-linked immunosorbent assay (ELISA) to detect antibodies against Fasciola spp. for diagnosis of human fasciolosis. The ELISA showed a high sensitivity (100%) and specificity (97.67%) when tested on patients with fasciolosis, other parasitic infections, cholangiocarcinoma and on healthy controls. The assay was applied for diagnosis on 143 patients in the Viet Duc-Hanoi hospital who presented with clinical signs of liver disease and lesions in their livers as shown by imaging techniques. Antibodies were found in 37 (25.9%) of these patients, of whom only 3 shed Fasciola eggs in their stools (2.1%). The excellent response to triclabendazole treatment of 37 sero-positive patients confirmed the diagnosis of fasciolosis. This study demonstrated the diagnostic potential for human fasciolosis of the 27-kDa antigen ELISA. Fasciolosis should be considered in the differential diagnosis of hepatic disease in Vietnam.},
  author       = {Nguyen Thi, Giang Thanh and  Le, TH and De, NV and Doan, TT and  Dao, THT and Vercruysse, Jozef and Dorny, Pierre},
  issn         = {1365-3156},
  journal      = {TROPICAL MEDICINE & INTERNATIONAL HEALTH},
  keywords     = {diagnosis,Fasciolosis,Enzyme-linked immunosorbent assay,hepatic disease,Vietnam,HEPATICA,GIGANTICA,SERODIAGNOSIS,CHOLANGIOCARCINOMA,TRICLABENDAZOLE,SHEEP},
  language     = {eng},
  number       = {4},
  pages        = {462--467},
  title        = {Assessment of a 27-kDa antigen in Enzyme-Linked Immunosorbent Assay for the diagnosis of fasciolosis in Vietnamese patients},
  url          = {http://dx.doi.org/10.1111/j.1365-3156.2010.02468.x},
  volume       = {15},
  year         = {2010},
}

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