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Use of Pseudomonas species producing phenazine-based metabolites in the anodes of microbial fuel cells to improve electricity generation

Hai The Pham UGent, Nico Boon UGent, Katrien De Maeyer UGent, Monica Höfte UGent, Korneel Rabaey UGent and Willy Verstraete UGent (2008) APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 80(6). p.985-993
abstract
The rate of anodic electron transfer is one of the factors limiting the performance of microbial fuel cells (MFCs). It is known that phenazine-based metabolites produced by Pseudomonas species can function as electron shuttles for Pseudomonas themselves and also, in a syntrophic association, for Gram-positive bacteria. In this study, we have investigated whether phenazine-based metabolites and their producers could be used to improve the electricity generation of a MFC operated with a mixed culture. Both anodic supernatants obtained from MFCs operated with a Pseudomonas strain (P-PCA) producing phenazine-1-carboxylic acid (PCA) and those from MFCs operated with a strain (P-PCN) producing phenazine-1-carboxamide (PCN) exerted similarly positive effects on the electricity generation of a mixed culture. Replacing supernatants of MFCs operated with a mixed culture with supernatants of MFCs operated with P-PCN could double the currents generated. Purified PCA and purified PCN had similar effects. If the supernatant of an engineered strain overproducing PCN was used, the effect could be maintained over longer time courses, resulting in a 1.5-fold increase in the production of charge. Bioaugmentation of the mixed culture MFCs using slow release tubes containing P-PCN not only doubled the currents but also maintained the effect over longer periods. The results demonstrated the electron-shuttling effect of phenazine-based compounds produced by Pseudomonas species and their capacity to improve the performance of MFCs operated with mixed cultures.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
COMMUNITIES, BIOSYNTHESIS, ANTIBIOTICS, NANOWIRES, phenazines, bacterial interactions, mixed culture, electrochemically active bacteria, microbial fuel cell, PHENAZINE-1-CARBOXAMIDE, BIOFUEL CELLS, CHLORORAPHIS PCL1391, EXTRACELLULAR ELECTRON-TRANSFER, MICROORGANISMS, BIOCONTROL
journal title
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Appl. Microbiol. Biotechnol.
volume
80
issue
6
pages
985 - 993
Web of Science type
Article
Web of Science id
000259670500005
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
2.569 (2008)
JCR rank
50/144 (2008)
JCR quartile
2 (2008)
ISSN
0175-7598
DOI
10.1007/s00253-008-1619-7
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
883331
handle
http://hdl.handle.net/1854/LU-883331
date created
2010-02-26 09:44:11
date last changed
2014-01-08 15:38:50
@article{883331,
  abstract     = {The rate of anodic electron transfer is one of the factors limiting the performance of microbial fuel cells (MFCs). It is known that phenazine-based metabolites produced by Pseudomonas species can function as electron shuttles for Pseudomonas themselves and also, in a syntrophic association, for Gram-positive bacteria. In this study, we have investigated whether phenazine-based metabolites and their producers could be used to improve the electricity generation of a MFC operated with a mixed culture. Both anodic supernatants obtained from MFCs operated with a Pseudomonas strain (P-PCA) producing phenazine-1-carboxylic acid (PCA) and those from MFCs operated with a strain (P-PCN) producing phenazine-1-carboxamide (PCN) exerted similarly positive effects on the electricity generation of a mixed culture. Replacing supernatants of MFCs operated with a mixed culture with supernatants of MFCs operated with P-PCN could double the currents generated. Purified PCA and purified PCN had similar effects. If the supernatant of an engineered strain overproducing PCN was used, the effect could be maintained over longer time courses, resulting in a 1.5-fold increase in the production of charge. Bioaugmentation of the mixed culture MFCs using slow release tubes containing P-PCN not only doubled the currents but also maintained the effect over longer periods. The results demonstrated the electron-shuttling effect of phenazine-based compounds produced by Pseudomonas species and their capacity to improve the performance of MFCs operated with mixed cultures.},
  author       = {Pham, Hai The and Boon, Nico and De Maeyer, Katrien and H{\"o}fte, Monica and Rabaey, Korneel and Verstraete, Willy},
  issn         = {0175-7598},
  journal      = {APPLIED MICROBIOLOGY AND BIOTECHNOLOGY},
  keyword      = {COMMUNITIES,BIOSYNTHESIS,ANTIBIOTICS,NANOWIRES,phenazines,bacterial interactions,mixed culture,electrochemically active bacteria,microbial fuel cell,PHENAZINE-1-CARBOXAMIDE,BIOFUEL CELLS,CHLORORAPHIS PCL1391,EXTRACELLULAR ELECTRON-TRANSFER,MICROORGANISMS,BIOCONTROL},
  language     = {eng},
  number       = {6},
  pages        = {985--993},
  title        = {Use of Pseudomonas species producing phenazine-based metabolites in the anodes of microbial fuel cells to improve electricity generation},
  url          = {http://dx.doi.org/10.1007/s00253-008-1619-7},
  volume       = {80},
  year         = {2008},
}

Chicago
Pham, Hai The, Nico Boon, Katrien De Maeyer, Monica Höfte, Korneel Rabaey, and Willy Verstraete. 2008. “Use of Pseudomonas Species Producing Phenazine-based Metabolites in the Anodes of Microbial Fuel Cells to Improve Electricity Generation.” Applied Microbiology and Biotechnology 80 (6): 985–993.
APA
Pham, H. T., Boon, N., De Maeyer, K., Höfte, M., Rabaey, K., & Verstraete, W. (2008). Use of Pseudomonas species producing phenazine-based metabolites in the anodes of microbial fuel cells to improve electricity generation. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 80(6), 985–993.
Vancouver
1.
Pham HT, Boon N, De Maeyer K, Höfte M, Rabaey K, Verstraete W. Use of Pseudomonas species producing phenazine-based metabolites in the anodes of microbial fuel cells to improve electricity generation. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2008;80(6):985–93.
MLA
Pham, Hai The, Nico Boon, Katrien De Maeyer, et al. “Use of Pseudomonas Species Producing Phenazine-based Metabolites in the Anodes of Microbial Fuel Cells to Improve Electricity Generation.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 80.6 (2008): 985–993. Print.