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Description of Acinetobacter venetianus ex Di Cello et al. 1997 sp nov.

Mario Vaneechoutte UGent, A Nemec, M Musilek, TJK van der Reijdend, M van den Barselaar, I Tjernberg, W Calame, R Fani, Thierry De Baere UGent and L Dijkshoorn (2009) INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 59. p.1376-1381
abstract
The name 'Acinetobacter venetianus' has been used previously to designate three marine hydrocarbon-degrading Acinetobacter strains, of which strain RAG-1 (=ATCC 31012) has industrial applications for the production of the bioemulsifier emulsan. However, to date, the name of this taxon has not been validly published. In this study, five strains were examined to corroborate the delineation of this taxon by means of phenotypic characterization, DNA-DNA hybridization, selective restriction fragment amplification (AFLP), amplified rDNA restriction analysis (ARDRA), rpoB gene sequence analysis and tRNA intergenic spacer length polymorphism analysis (tDNA-PCR) and to emend the description of 'Acinetobacter venetianus' (ex Di Cello et al. 1997). AFLP analysis showed that the five strains formed a tight cluster at 56.8 +/- 5.0% genomic relatedness that was separated from strains of other haemolytic species of the genus Acinetobacter and from the type and reference strains of other Acinetobacter species at <= 27% relatedness, indicating the distinctiveness of the novel strains. The strains were haemolytic and able to grow on citrate (Simmons), L-histidine and malonate. The strains did not oxidize D-glucose or utilize DL-lactate or L-aspartate. The G + C contents of strains RAG-1 and of VE-C3 were 43.9% and 43.6 mol%, respectively. The novel strains could be recognized by a characteristic ARDRA pattern (Cfol 1, Alul 3, Mbol 2, Rsal 2, Mspl 3). The consensus tDNA-PCR pattern for the five strains consisted of amplified fragments of 87.9, 100.2, 134.6 and 248.5 bp and was indistinguishable from that of strains of Acinetobacter genomic species 14BJ. The five strains represent a novel species for which the name Acinetobacter venetianus sp. nov. is proposed. The type strain is RAG-1(T) (=ATCC 31012(T)=CCUG 45561(T)=LMG 19082(T)=LUH 3904(T)=NIPH 1925(T)).
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
OIL, HYBRIDIZATION, RAG-1, DNA RESTRICTION ANALYSIS, HUMAN CLINICAL SPECIMENS, GENUS ACINETOBACTER, CARBON SOURCE, IDENTIFICATION, STRAINS, CALCOACETICUS
journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Int. J. Syst. Evol. Microbiol.
volume
59
pages
6 pages
publisher
SOC GENERAL MICROBIOLOGY
place of publication
READING
Web of Science type
Article
Web of Science id
000267645000022
JCR category
MICROBIOLOGY
JCR impact factor
2.113 (2009)
JCR rank
56/94 (2009)
JCR quartile
3 (2009)
ISSN
1466-5026
DOI
10.1099/ijs.0.003541-0
language
English
UGent publication?
yes
classification
A1
id
879940
handle
http://hdl.handle.net/1854/LU-879940
date created
2010-02-24 16:51:59
date last changed
2010-02-25 14:32:10
@article{879940,
  abstract     = {The name 'Acinetobacter venetianus' has been used previously to designate three marine hydrocarbon-degrading Acinetobacter strains, of which strain RAG-1 (=ATCC 31012) has industrial applications for the production of the bioemulsifier emulsan. However, to date, the name of this taxon has not been validly published. In this study, five strains were examined to corroborate the delineation of this taxon by means of phenotypic characterization, DNA-DNA hybridization, selective restriction fragment amplification (AFLP), amplified rDNA restriction analysis (ARDRA), rpoB gene sequence analysis and tRNA intergenic spacer length polymorphism analysis (tDNA-PCR) and to emend the description of 'Acinetobacter venetianus' (ex Di Cello et al. 1997). AFLP analysis showed that the five strains formed a tight cluster at 56.8 +/- 5.0\% genomic relatedness that was separated from strains of other haemolytic species of the genus Acinetobacter and from the type and reference strains of other Acinetobacter species at {\textlangle}= 27\% relatedness, indicating the distinctiveness of the novel strains. The strains were haemolytic and able to grow on citrate (Simmons), L-histidine and malonate. The strains did not oxidize D-glucose or utilize DL-lactate or L-aspartate. The G + C contents of strains RAG-1 and of VE-C3 were 43.9\% and 43.6 mol\%, respectively. The novel strains could be recognized by a characteristic ARDRA pattern (Cfol 1, Alul 3, Mbol 2, Rsal 2, Mspl 3). The consensus tDNA-PCR pattern for the five strains consisted of amplified fragments of 87.9, 100.2, 134.6 and 248.5 bp and was indistinguishable from that of strains of Acinetobacter genomic species 14BJ. The five strains represent a novel species for which the name Acinetobacter venetianus sp. nov. is proposed. The type strain is RAG-1(T) (=ATCC 31012(T)=CCUG 45561(T)=LMG 19082(T)=LUH 3904(T)=NIPH 1925(T)).},
  author       = {Vaneechoutte, Mario and Nemec, A and Musilek, M and van der Reijdend, TJK and van den Barselaar, M and Tjernberg, I and Calame, W and Fani, R and De Baere, Thierry and Dijkshoorn, L},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {OIL,HYBRIDIZATION,RAG-1,DNA RESTRICTION ANALYSIS,HUMAN CLINICAL SPECIMENS,GENUS ACINETOBACTER,CARBON SOURCE,IDENTIFICATION,STRAINS,CALCOACETICUS},
  language     = {eng},
  pages        = {1376--1381},
  publisher    = {SOC GENERAL MICROBIOLOGY},
  title        = {Description of Acinetobacter venetianus ex Di Cello et al. 1997 sp nov.},
  url          = {http://dx.doi.org/10.1099/ijs.0.003541-0},
  volume       = {59},
  year         = {2009},
}

Chicago
Vaneechoutte, Mario, A Nemec, M Musilek, TJK van der Reijdend, M van den Barselaar, I Tjernberg, W Calame, R Fani, Thierry De Baere, and L Dijkshoorn. 2009. “Description of Acinetobacter Venetianus Ex Di Cello Et Al. 1997 Sp Nov.” International Journal of Systematic and Evolutionary Microbiology 59: 1376–1381.
APA
Vaneechoutte, M., Nemec, A., Musilek, M., van der Reijdend, T., van den Barselaar, M., Tjernberg, I., Calame, W., et al. (2009). Description of Acinetobacter venetianus ex Di Cello et al. 1997 sp nov. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 59, 1376–1381.
Vancouver
1.
Vaneechoutte M, Nemec A, Musilek M, van der Reijdend T, van den Barselaar M, Tjernberg I, et al. Description of Acinetobacter venetianus ex Di Cello et al. 1997 sp nov. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. READING: SOC GENERAL MICROBIOLOGY; 2009;59:1376–81.
MLA
Vaneechoutte, Mario, A Nemec, M Musilek, et al. “Description of Acinetobacter Venetianus Ex Di Cello Et Al. 1997 Sp Nov.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 59 (2009): 1376–1381. Print.