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Survey of Pseudomonas aeruginosa and its phages: de novo peptide sequencing as a novel tool to assess the diversity of worldwide collected viruses

Pieter-Jan Ceyssens, Jean-Paul Noben, Hans-W Ackermann, Jan Verhaegen, Daniel De Vos, Jean-Paul Pirnay, Maia Merabishvili UGent, Mario Vaneechoutte UGent, Andrew Chibeu and Guido Volckaert, et al. (2009) ENVIRONMENTAL MICROBIOLOGY. 11(5). p.1303-1313
abstract
A collection of 15 newly isolated (bacterio)phages infecting the opportunistic pathogen Pseudomonas aeruginosa was established to investigate their global diversity and potential in phage therapy. These phages were sampled in 14 different countries traversing four continents, from both natural environments and hospital sewage. They all display unique DNA and protein profiles and cluster morphologically into six groups within the three major families of the Caudovirales. Extensive host range studies on a library of 122 AFLP-genotyped clinical P. aeruginosa strains (of which 49 were newly isolated at the University Hospital of Leuven, Belgium) showed that the phages lysed 87% of the strains. Infection analysis of outer membrane mutants identified 10 phages as type IV pili-dependent. More detailed information about the evolutionary relatedness of the phages was gathered by de novo peptide sequencing of major virion proteins using tandem Matrix-Assisted Laser Desorption/Ionization Time of Flight technology. Applying this technique for the first time to viruses, seven groups of closely related phages were identified without the need of prior knowledge of genome content and/or electron microscopic imaging. This study demonstrates both the epidemic population structure of P. aeruginosa and the global spread of P. aeruginosa phage species, and points at the resistance of two clinically predominant, widespread P. aeruginosa strains against phage attack.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DERIVATIZATION, IDENTIFICATION, MALDI, BACTERIOPHAGES, PHI-KMV, STRUCTURAL PROTEOME, GENOME SEQUENCE, DNA PACKAGING STRATEGY, FLIGHT MASS-SPECTROMETRY, CYSTIC-FIBROSIS PATIENTS
journal title
ENVIRONMENTAL MICROBIOLOGY
Environ. Microbiol.
volume
11
issue
5
pages
1303 - 1313
Web of Science type
Article
Web of Science id
000265481600022
JCR category
MICROBIOLOGY
JCR impact factor
4.909 (2009)
JCR rank
15/94 (2009)
JCR quartile
1 (2009)
ISSN
1462-2912
DOI
10.1111/j.1462-2920.2008.01862.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
879853
handle
http://hdl.handle.net/1854/LU-879853
date created
2010-02-24 16:50:34
date last changed
2010-09-15 16:43:58
@article{879853,
  abstract     = {A collection of 15 newly isolated (bacterio)phages infecting the opportunistic pathogen Pseudomonas aeruginosa was established to investigate their global diversity and potential in phage therapy. These phages were sampled in 14 different countries traversing four continents, from both natural environments and hospital sewage. They all display unique DNA and protein profiles and cluster morphologically into six groups within the three major families of the Caudovirales. Extensive host range studies on a library of 122 AFLP-genotyped clinical P. aeruginosa strains (of which 49 were newly isolated at the University Hospital of Leuven, Belgium) showed that the phages lysed 87\% of the strains. Infection analysis of outer membrane mutants identified 10 phages as type IV pili-dependent. More detailed information about the evolutionary relatedness of the phages was gathered by de novo peptide sequencing of major virion proteins using tandem Matrix-Assisted Laser Desorption/Ionization Time of Flight technology. Applying this technique for the first time to viruses, seven groups of closely related phages were identified without the need of prior knowledge of genome content and/or electron microscopic imaging. This study demonstrates both the epidemic population structure of P. aeruginosa and the global spread of P. aeruginosa phage species, and points at the resistance of two clinically predominant, widespread P. aeruginosa strains against phage attack.},
  author       = {Ceyssens, Pieter-Jan and Noben, Jean-Paul and Ackermann, Hans-W and Verhaegen, Jan and De Vos, Daniel and Pirnay, Jean-Paul and Merabishvili, Maia and Vaneechoutte, Mario and Chibeu, Andrew and Volckaert, Guido and Lavigne, Rob},
  issn         = {1462-2912},
  journal      = {ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {DERIVATIZATION,IDENTIFICATION,MALDI,BACTERIOPHAGES,PHI-KMV,STRUCTURAL PROTEOME,GENOME SEQUENCE,DNA PACKAGING STRATEGY,FLIGHT MASS-SPECTROMETRY,CYSTIC-FIBROSIS PATIENTS},
  language     = {eng},
  number       = {5},
  pages        = {1303--1313},
  title        = {Survey of Pseudomonas aeruginosa and its phages: de novo peptide sequencing as a novel tool to assess the diversity of worldwide collected viruses},
  url          = {http://dx.doi.org/10.1111/j.1462-2920.2008.01862.x},
  volume       = {11},
  year         = {2009},
}

Chicago
Ceyssens, Pieter-Jan, Jean-Paul Noben, Hans-W Ackermann, Jan Verhaegen, Daniel De Vos, Jean-Paul Pirnay, Maia Merabishvili, et al. 2009. “Survey of Pseudomonas Aeruginosa and Its Phages: De Novo Peptide Sequencing as a Novel Tool to Assess the Diversity of Worldwide Collected Viruses.” Environmental Microbiology 11 (5): 1303–1313.
APA
Ceyssens, P.-J., Noben, J.-P., Ackermann, H.-W., Verhaegen, J., De Vos, D., Pirnay, J.-P., Merabishvili, M., et al. (2009). Survey of Pseudomonas aeruginosa and its phages: de novo peptide sequencing as a novel tool to assess the diversity of worldwide collected viruses. ENVIRONMENTAL MICROBIOLOGY, 11(5), 1303–1313.
Vancouver
1.
Ceyssens P-J, Noben J-P, Ackermann H-W, Verhaegen J, De Vos D, Pirnay J-P, et al. Survey of Pseudomonas aeruginosa and its phages: de novo peptide sequencing as a novel tool to assess the diversity of worldwide collected viruses. ENVIRONMENTAL MICROBIOLOGY. 2009;11(5):1303–13.
MLA
Ceyssens, Pieter-Jan, Jean-Paul Noben, Hans-W Ackermann, et al. “Survey of Pseudomonas Aeruginosa and Its Phages: De Novo Peptide Sequencing as a Novel Tool to Assess the Diversity of Worldwide Collected Viruses.” ENVIRONMENTAL MICROBIOLOGY 11.5 (2009): 1303–1313. Print.