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The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2

Ihab B Lolas, Kristiina Himanen (UGent) , Jesper T Gronlund, Carine Lynggaard, Andreas Houben, Michael Melzer, Maria Van Lijsebettens (UGent) and Klaus D Grasser
(2010) PLANT JOURNAL. 61(4). p.686-697
Author
Organization
Abstract
The facilitates chromatin transcription ( FACT) complex, consisting of the SSRP1 and SPT16 proteins, is a histone chaperone that assists the progression of transcribing RNA polymerase on chromatin templates by destabilizing nucleosomes. Here, we examined plants that harbour mutations in the genes encoding the subunits of Arabidopsis FACT. These experiments revealed that (i) SSRP1 is critical for plant viability, and (ii) plants with reduced amounts of SSRP1 and SPT16 display various defects in vegetative and reproductive development. Thus, mutant plants display an increased number of leaves and inflorescences, show early bolting, have abnormal flower and leaf architecture, and their seed production is severely affected. The early flowering of the mutant plants is associated with reduced expression of the floral repressor FLC in ssrp1 and spt16 plants. Compared to control plants, reduced amounts of FACT in mutant plants are detected at the FLC locus as well as at the locations of housekeeping genes (whose expression is not affected in the mutants), suggesting that expression of FLC is particularly sensitive to reduced FACT activity. Analysis of double mutants that are affected in the expression of both FACT subunits and factors catalysing the mono-ubiquitination of histone H2B (HUB1/2) demonstrates that they genetically interact to regulate various developmental processes (i.e. branching, leaf venation pattern, silique development) but independently regulate the growth of leaves and the induction of flowering.
Keywords
GENE, REPRESSION, FLORAL TRANSITION, CHROMATIN REMODELING COMPLEX, HISTONE H2B MONOUBIQUITINATION, DOMAIN PROTEIN SSRP1, RNA-POLYMERASE-II, PLANT DEVELOPMENT, NUCLEOSOME DYNAMICS, FLOWERING-TIME

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MLA
Lolas, Ihab B., et al. “The Transcript Elongation Factor FACT Affects Arabidopsis Vegetative and Reproductive Development and Genetically Interacts with HUB1/2.” PLANT JOURNAL, vol. 61, no. 4, WILEY-BLACKWELL PUBLISHING, INC, 2010, pp. 686–97, doi:10.1111/j.1365-313X.2009.04096.x.
APA
Lolas, I. B., Himanen, K., Gronlund, J. T., Lynggaard, C., Houben, A., Melzer, M., … Grasser, K. D. (2010). The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2. PLANT JOURNAL, 61(4), 686–697. https://doi.org/10.1111/j.1365-313X.2009.04096.x
Chicago author-date
Lolas, Ihab B, Kristiina Himanen, Jesper T Gronlund, Carine Lynggaard, Andreas Houben, Michael Melzer, Maria Van Lijsebettens, and Klaus D Grasser. 2010. “The Transcript Elongation Factor FACT Affects Arabidopsis Vegetative and Reproductive Development and Genetically Interacts with HUB1/2.” PLANT JOURNAL 61 (4): 686–97. https://doi.org/10.1111/j.1365-313X.2009.04096.x.
Chicago author-date (all authors)
Lolas, Ihab B, Kristiina Himanen, Jesper T Gronlund, Carine Lynggaard, Andreas Houben, Michael Melzer, Maria Van Lijsebettens, and Klaus D Grasser. 2010. “The Transcript Elongation Factor FACT Affects Arabidopsis Vegetative and Reproductive Development and Genetically Interacts with HUB1/2.” PLANT JOURNAL 61 (4): 686–697. doi:10.1111/j.1365-313X.2009.04096.x.
Vancouver
1.
Lolas IB, Himanen K, Gronlund JT, Lynggaard C, Houben A, Melzer M, et al. The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2. PLANT JOURNAL. 2010;61(4):686–97.
IEEE
[1]
I. B. Lolas et al., “The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2,” PLANT JOURNAL, vol. 61, no. 4, pp. 686–697, 2010.
@article{878811,
  abstract     = {{The facilitates chromatin transcription ( FACT) complex, consisting of the SSRP1 and SPT16 proteins, is a histone chaperone that assists the progression of transcribing RNA polymerase on chromatin templates by destabilizing nucleosomes. Here, we examined plants that harbour mutations in the genes encoding the subunits of Arabidopsis FACT. These experiments revealed that (i) SSRP1 is critical for plant viability, and (ii) plants with reduced amounts of SSRP1 and SPT16 display various defects in vegetative and reproductive development. Thus, mutant plants display an increased number of leaves and inflorescences, show early bolting, have abnormal flower and leaf architecture, and their seed production is severely affected. The early flowering of the mutant plants is associated with reduced expression of the floral repressor FLC in ssrp1 and spt16 plants. Compared to control plants, reduced amounts of FACT in mutant plants are detected at the FLC locus as well as at the locations of housekeeping genes (whose expression is not affected in the mutants), suggesting that expression of FLC is particularly sensitive to reduced FACT activity. Analysis of double mutants that are affected in the expression of both FACT subunits and factors catalysing the mono-ubiquitination of histone H2B (HUB1/2) demonstrates that they genetically interact to regulate various developmental processes (i.e. branching, leaf venation pattern, silique development) but independently regulate the growth of leaves and the induction of flowering.}},
  author       = {{Lolas, Ihab B and Himanen, Kristiina and Gronlund, Jesper T and Lynggaard, Carine and Houben, Andreas and Melzer, Michael and Van Lijsebettens, Maria and Grasser, Klaus D}},
  issn         = {{0960-7412}},
  journal      = {{PLANT JOURNAL}},
  keywords     = {{GENE,REPRESSION,FLORAL TRANSITION,CHROMATIN REMODELING COMPLEX,HISTONE H2B MONOUBIQUITINATION,DOMAIN PROTEIN SSRP1,RNA-POLYMERASE-II,PLANT DEVELOPMENT,NUCLEOSOME DYNAMICS,FLOWERING-TIME}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{686--697}},
  publisher    = {{WILEY-BLACKWELL PUBLISHING, INC}},
  title        = {{The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2}},
  url          = {{http://dx.doi.org/10.1111/j.1365-313X.2009.04096.x}},
  volume       = {{61}},
  year         = {{2010}},
}
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