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Systematic localization of the Arabidopsis core cell cycle proteins reveals novel cell division complexes

Joanna Boruc (UGent) , Evelien Mylle (UGent) , Maria Duda (UGent) , Rebecca De Clercq (UGent) , Stephane Rombauts (UGent) , Danny Geelen (UGent) , Pierre Hilson (UGent) , Dirk Inzé (UGent) , Daniël Van Damme (UGent) and Eugenia Russinova (UGent)
(2010) PLANT PHYSIOLOGY. 152(2). p.553-565
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Abstract
Cell division depends on the correct localization of the cyclin-dependent kinases that are regulated by phosphorylation, cyclin proteolysis, and protein-protein interactions. Although immunological assays can define cell cycle protein abundance and localization, they are not suitable for detecting the dynamic rearrangements of molecular components during cell division. Here, we applied an in vivo approach to trace the subcellular localization of 60 Arabidopsis (Arabidopsis thaliana) core cell cycle proteins fused to green fluorescent proteins during cell division in tobacco (Nicotiana tabacum) and Arabidopsis. Several cell cycle proteins showed a dynamic association with mitotic structures, such as condensed chromosomes and the preprophase band in both species, suggesting a strong conservation of targeting mechanisms. Furthermore, colocalized proteins were shown to bind in vivo, strengthening their localization-function connection. Thus, we identified unknown spatiotemporal territories where functional cell cycle protein interactions are most likely to occur.
Keywords
DNA-REPLICATION, PREPROPHASE BAND, A-TYPE CYCLIN, GENE-EXPRESSION, GENOME-WIDE ANALYSIS, XENOPUS SUC1/CKS PROTEIN, DEPENDENT KINASE INHIBITORS, ANAPHASE-PROMOTING COMPLEX, INDEPENDENT FUNCTION, MICROTUBULE DYNAMICS

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MLA
Boruc, Joanna, et al. “Systematic Localization of the Arabidopsis Core Cell Cycle Proteins Reveals Novel Cell Division Complexes.” PLANT PHYSIOLOGY, vol. 152, no. 2, 2010, pp. 553–65, doi:10.1104/pp.109.148643.
APA
Boruc, J., Mylle, E., Duda, M., De Clercq, R., Rombauts, S., Geelen, D., … Russinova, E. (2010). Systematic localization of the Arabidopsis core cell cycle proteins reveals novel cell division complexes. PLANT PHYSIOLOGY, 152(2), 553–565. https://doi.org/10.1104/pp.109.148643
Chicago author-date
Boruc, Joanna, Evelien Mylle, Maria Duda, Rebecca De Clercq, Stephane Rombauts, Danny Geelen, Pierre Hilson, Dirk Inzé, Daniël Van Damme, and Eugenia Russinova. 2010. “Systematic Localization of the Arabidopsis Core Cell Cycle Proteins Reveals Novel Cell Division Complexes.” PLANT PHYSIOLOGY 152 (2): 553–65. https://doi.org/10.1104/pp.109.148643.
Chicago author-date (all authors)
Boruc, Joanna, Evelien Mylle, Maria Duda, Rebecca De Clercq, Stephane Rombauts, Danny Geelen, Pierre Hilson, Dirk Inzé, Daniël Van Damme, and Eugenia Russinova. 2010. “Systematic Localization of the Arabidopsis Core Cell Cycle Proteins Reveals Novel Cell Division Complexes.” PLANT PHYSIOLOGY 152 (2): 553–565. doi:10.1104/pp.109.148643.
Vancouver
1.
Boruc J, Mylle E, Duda M, De Clercq R, Rombauts S, Geelen D, et al. Systematic localization of the Arabidopsis core cell cycle proteins reveals novel cell division complexes. PLANT PHYSIOLOGY. 2010;152(2):553–65.
IEEE
[1]
J. Boruc et al., “Systematic localization of the Arabidopsis core cell cycle proteins reveals novel cell division complexes,” PLANT PHYSIOLOGY, vol. 152, no. 2, pp. 553–565, 2010.
@article{878786,
  abstract     = {{Cell division depends on the correct localization of the cyclin-dependent kinases that are regulated by phosphorylation, cyclin proteolysis, and protein-protein interactions. Although immunological assays can define cell cycle protein abundance and localization, they are not suitable for detecting the dynamic rearrangements of molecular components during cell division. Here, we applied an in vivo approach to trace the subcellular localization of 60 Arabidopsis (Arabidopsis thaliana) core cell cycle proteins fused to green fluorescent proteins during cell division in tobacco (Nicotiana tabacum) and Arabidopsis. Several cell cycle proteins showed a dynamic association with mitotic structures, such as condensed chromosomes and the preprophase band in both species, suggesting a strong conservation of targeting mechanisms. Furthermore, colocalized proteins were shown to bind in vivo, strengthening their localization-function connection. Thus, we identified unknown spatiotemporal territories where functional cell cycle protein interactions are most likely to occur.}},
  author       = {{Boruc, Joanna and Mylle, Evelien and Duda, Maria and De Clercq, Rebecca and Rombauts, Stephane and Geelen, Danny and Hilson, Pierre and Inzé, Dirk and Van Damme, Daniël and Russinova, Eugenia}},
  issn         = {{0032-0889}},
  journal      = {{PLANT PHYSIOLOGY}},
  keywords     = {{DNA-REPLICATION,PREPROPHASE BAND,A-TYPE CYCLIN,GENE-EXPRESSION,GENOME-WIDE ANALYSIS,XENOPUS SUC1/CKS PROTEIN,DEPENDENT KINASE INHIBITORS,ANAPHASE-PROMOTING COMPLEX,INDEPENDENT FUNCTION,MICROTUBULE DYNAMICS}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{553--565}},
  title        = {{Systematic localization of the Arabidopsis core cell cycle proteins reveals novel cell division complexes}},
  url          = {{http://dx.doi.org/10.1104/pp.109.148643}},
  volume       = {{152}},
  year         = {{2010}},
}

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