The MCM-Binding Protein ETG1 Aids Sister Chromatid Cohesion Required for Postreplicative Homologous Recombination Repair
- Author
- Naoki Takahashi (UGent) , Mauricio Quimbaya Gomez (UGent) , Veit Schubert, Tim Lammens (UGent) , Klaas Vandepoele (UGent) , Ingo Schubert, Minami Matsui, Dirk Inzé (UGent) , Geert Berx (UGent) and Lieven De Veylder (UGent)
- Organization
- Abstract
- The DNA replication process represents a source of DNA stress that causes potentially spontaneous genome damage. This effect might be strengthened by mutations in crucial replication factors, requiring the activation of DNA damage checkpoints to enable DNA repair before anaphase onset. Here, we demonstrate that depletion of the evolutionarily conserved minichromosome maintenance helicase-binding protein ETG1 of Arabidopsis thaliana resulted in a stringent late G2 cell cycle arrest. This arrest correlated with a partial loss of sister chromatid cohesion. The lack-of-cohesion phenotype was intensified in plants without functional CTF18, a replication fork factor needed for cohesion establishment. The synergistic effect of the etg1 and ctf18 mutants on sister chromatid cohesion strengthened the impact on plant growth of the replication stress caused by ETG1 deficiency because of inefficient DNA repair. We conclude that the ETG1 replication factor is required for efficient cohesion and that cohesion establishment is essential for proper development of plants suffering from endogenous DNA stress. Cohesion defects observed upon knockdown of its human counterpart suggest an equally important developmental role for the orthologous mammalian ETG1 protein.
- Keywords
- DNA-DAMAGE REPAIR, SACCHAROMYCES-CEREVISIAE, ARABIDOPSIS-THALIANA, STRUCTURAL MAINTENANCE, MEDIATED TRANSFORMATION, IONIZING-RADIATION, ATP HYDROLYSIS, INTERPHASE NUCLEI, SMC PROTEINS, S-PHASE
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-878774
- MLA
- Takahashi, Naoki, et al. “The MCM-Binding Protein ETG1 Aids Sister Chromatid Cohesion Required for Postreplicative Homologous Recombination Repair.” PLOS GENETICS, vol. 6, no. 1, PUBLIC LIBRARY SCIENCE, 2010, doi:10.1371/journal.pgen.1000817.
- APA
- Takahashi, N., Quimbaya Gomez, M., Schubert, V., Lammens, T., Vandepoele, K., Schubert, I., … De Veylder, L. (2010). The MCM-Binding Protein ETG1 Aids Sister Chromatid Cohesion Required for Postreplicative Homologous Recombination Repair. PLOS GENETICS, 6(1). https://doi.org/10.1371/journal.pgen.1000817
- Chicago author-date
- Takahashi, Naoki, Mauricio Quimbaya Gomez, Veit Schubert, Tim Lammens, Klaas Vandepoele, Ingo Schubert, Minami Matsui, Dirk Inzé, Geert Berx, and Lieven De Veylder. 2010. “The MCM-Binding Protein ETG1 Aids Sister Chromatid Cohesion Required for Postreplicative Homologous Recombination Repair.” PLOS GENETICS 6 (1). https://doi.org/10.1371/journal.pgen.1000817.
- Chicago author-date (all authors)
- Takahashi, Naoki, Mauricio Quimbaya Gomez, Veit Schubert, Tim Lammens, Klaas Vandepoele, Ingo Schubert, Minami Matsui, Dirk Inzé, Geert Berx, and Lieven De Veylder. 2010. “The MCM-Binding Protein ETG1 Aids Sister Chromatid Cohesion Required for Postreplicative Homologous Recombination Repair.” PLOS GENETICS 6 (1). doi:10.1371/journal.pgen.1000817.
- Vancouver
- 1.Takahashi N, Quimbaya Gomez M, Schubert V, Lammens T, Vandepoele K, Schubert I, et al. The MCM-Binding Protein ETG1 Aids Sister Chromatid Cohesion Required for Postreplicative Homologous Recombination Repair. PLOS GENETICS. 2010;6(1).
- IEEE
- [1]N. Takahashi et al., “The MCM-Binding Protein ETG1 Aids Sister Chromatid Cohesion Required for Postreplicative Homologous Recombination Repair,” PLOS GENETICS, vol. 6, no. 1, 2010.
@article{878774,
abstract = {{The DNA replication process represents a source of DNA stress that causes potentially spontaneous genome damage. This effect might be strengthened by mutations in crucial replication factors, requiring the activation of DNA damage checkpoints to enable DNA repair before anaphase onset. Here, we demonstrate that depletion of the evolutionarily conserved minichromosome maintenance helicase-binding protein ETG1 of Arabidopsis thaliana resulted in a stringent late G2 cell cycle arrest. This arrest correlated with a partial loss of sister chromatid cohesion. The lack-of-cohesion phenotype was intensified in plants without functional CTF18, a replication fork factor needed for cohesion establishment. The synergistic effect of the etg1 and ctf18 mutants on sister chromatid cohesion strengthened the impact on plant growth of the replication stress caused by ETG1 deficiency because of inefficient DNA repair. We conclude that the ETG1 replication factor is required for efficient cohesion and that cohesion establishment is essential for proper development of plants suffering from endogenous DNA stress. Cohesion defects observed upon knockdown of its human counterpart suggest an equally important developmental role for the orthologous mammalian ETG1 protein.}},
author = {{Takahashi, Naoki and Quimbaya Gomez, Mauricio and Schubert, Veit and Lammens, Tim and Vandepoele, Klaas and Schubert, Ingo and Matsui, Minami and Inzé, Dirk and Berx, Geert and De Veylder, Lieven}},
issn = {{1553-7390}},
journal = {{PLOS GENETICS}},
keywords = {{DNA-DAMAGE REPAIR,SACCHAROMYCES-CEREVISIAE,ARABIDOPSIS-THALIANA,STRUCTURAL MAINTENANCE,MEDIATED TRANSFORMATION,IONIZING-RADIATION,ATP HYDROLYSIS,INTERPHASE NUCLEI,SMC PROTEINS,S-PHASE}},
language = {{eng}},
number = {{1}},
pages = {{13}},
publisher = {{PUBLIC LIBRARY SCIENCE}},
title = {{The MCM-Binding Protein ETG1 Aids Sister Chromatid Cohesion Required for Postreplicative Homologous Recombination Repair}},
url = {{http://dx.doi.org/10.1371/journal.pgen.1000817}},
volume = {{6}},
year = {{2010}},
}
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