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The MCM-Binding Protein ETG1 Aids Sister Chromatid Cohesion Required for Postreplicative Homologous Recombination Repair

Naoki Takahashi UGent, Mauricio Alberto Quimbaya Gomez UGent, Veit Schubert, Tim Lammens UGent, Klaas Vandepoele UGent, Ingo Schubert, Minami Matsui, Dirk Inzé UGent, Geert Berx UGent and Lieven De Veylder UGent (2010) PLOS GENETICS. 6(1).
abstract
The DNA replication process represents a source of DNA stress that causes potentially spontaneous genome damage. This effect might be strengthened by mutations in crucial replication factors, requiring the activation of DNA damage checkpoints to enable DNA repair before anaphase onset. Here, we demonstrate that depletion of the evolutionarily conserved minichromosome maintenance helicase-binding protein ETG1 of Arabidopsis thaliana resulted in a stringent late G2 cell cycle arrest. This arrest correlated with a partial loss of sister chromatid cohesion. The lack-of-cohesion phenotype was intensified in plants without functional CTF18, a replication fork factor needed for cohesion establishment. The synergistic effect of the etg1 and ctf18 mutants on sister chromatid cohesion strengthened the impact on plant growth of the replication stress caused by ETG1 deficiency because of inefficient DNA repair. We conclude that the ETG1 replication factor is required for efficient cohesion and that cohesion establishment is essential for proper development of plants suffering from endogenous DNA stress. Cohesion defects observed upon knockdown of its human counterpart suggest an equally important developmental role for the orthologous mammalian ETG1 protein.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DNA-DAMAGE REPAIR, SACCHAROMYCES-CEREVISIAE, ARABIDOPSIS-THALIANA, STRUCTURAL MAINTENANCE, MEDIATED TRANSFORMATION, IONIZING-RADIATION, ATP HYDROLYSIS, INTERPHASE NUCLEI, SMC PROTEINS, S-PHASE
journal title
PLOS GENETICS
PLoS Genet.
volume
6
issue
1
pages
13 pages
publisher
PUBLIC LIBRARY SCIENCE
place of publication
SAN FRANCISCO
Web of Science type
Article
Web of Science id
000274194300025
JCR category
GENETICS & HEREDITY
JCR impact factor
9.543 (2010)
JCR rank
10/154 (2010)
JCR quartile
1 (2010)
ISSN
1553-7390
DOI
10.1371/journal.pgen.1000817
language
English
UGent publication?
yes
classification
A1
copyright statement
I don't know the status of the copyright for this publication
id
878774
handle
http://hdl.handle.net/1854/LU-878774
date created
2010-02-24 11:41:05
date last changed
2012-06-26 14:31:58
@article{878774,
  abstract     = {The DNA replication process represents a source of DNA stress that causes potentially spontaneous genome damage. This effect might be strengthened by mutations in crucial replication factors, requiring the activation of DNA damage checkpoints to enable DNA repair before anaphase onset. Here, we demonstrate that depletion of the evolutionarily conserved minichromosome maintenance helicase-binding protein ETG1 of Arabidopsis thaliana resulted in a stringent late G2 cell cycle arrest. This arrest correlated with a partial loss of sister chromatid cohesion. The lack-of-cohesion phenotype was intensified in plants without functional CTF18, a replication fork factor needed for cohesion establishment. The synergistic effect of the etg1 and ctf18 mutants on sister chromatid cohesion strengthened the impact on plant growth of the replication stress caused by ETG1 deficiency because of inefficient DNA repair. We conclude that the ETG1 replication factor is required for efficient cohesion and that cohesion establishment is essential for proper development of plants suffering from endogenous DNA stress. Cohesion defects observed upon knockdown of its human counterpart suggest an equally important developmental role for the orthologous mammalian ETG1 protein.},
  author       = {Takahashi, Naoki and Quimbaya Gomez, Mauricio Alberto and Schubert, Veit and Lammens, Tim and Vandepoele, Klaas and Schubert, Ingo and Matsui, Minami and Inz{\'e}, Dirk and Berx, Geert and De Veylder, Lieven},
  issn         = {1553-7390},
  journal      = {PLOS GENETICS},
  keyword      = {DNA-DAMAGE REPAIR,SACCHAROMYCES-CEREVISIAE,ARABIDOPSIS-THALIANA,STRUCTURAL MAINTENANCE,MEDIATED TRANSFORMATION,IONIZING-RADIATION,ATP HYDROLYSIS,INTERPHASE NUCLEI,SMC PROTEINS,S-PHASE},
  language     = {eng},
  number       = {1},
  pages        = {13},
  publisher    = {PUBLIC LIBRARY SCIENCE},
  title        = {The MCM-Binding Protein ETG1 Aids Sister Chromatid Cohesion Required for Postreplicative Homologous Recombination Repair},
  url          = {http://dx.doi.org/10.1371/journal.pgen.1000817},
  volume       = {6},
  year         = {2010},
}

Chicago
Takahashi, Naoki, Mauricio Alberto Quimbaya Gomez, Veit Schubert, Tim Lammens, Klaas Vandepoele, Ingo Schubert, Minami Matsui, Dirk Inzé, Geert Berx, and Lieven De Veylder. 2010. “The MCM-Binding Protein ETG1 Aids Sister Chromatid Cohesion Required for Postreplicative Homologous Recombination Repair.” Plos Genetics 6 (1).
APA
Takahashi, Naoki, Quimbaya Gomez, M. A., Schubert, V., Lammens, T., Vandepoele, K., Schubert, I., Matsui, M., et al. (2010). The MCM-Binding Protein ETG1 Aids Sister Chromatid Cohesion Required for Postreplicative Homologous Recombination Repair. PLOS GENETICS, 6(1).
Vancouver
1.
Takahashi N, Quimbaya Gomez MA, Schubert V, Lammens T, Vandepoele K, Schubert I, et al. The MCM-Binding Protein ETG1 Aids Sister Chromatid Cohesion Required for Postreplicative Homologous Recombination Repair. PLOS GENETICS. SAN FRANCISCO: PUBLIC LIBRARY SCIENCE; 2010;6(1).
MLA
Takahashi, Naoki, Mauricio Alberto Quimbaya Gomez, Veit Schubert, et al. “The MCM-Binding Protein ETG1 Aids Sister Chromatid Cohesion Required for Postreplicative Homologous Recombination Repair.” PLOS GENETICS 6.1 (2010): n. pag. Print.