Academic Bibliography

 Search 200 years of publications by Ghent University researchers.
Advanced search
1 file | 9.85 MB
Add to list
  1. Search results
  2. Dry Ice Self-Pressurised Rapid Freezi...

Dry Ice Self-Pressurised Rapid Freezing (DryIce SPRF): all you need to cool for cryofixation of nematodes is dry ice

Myriam Claeys (UGent) , Vladimir V. Yushin and Wim Bert (UGent)
(2021) NEMATOLOGY. 23(10). p.1179-1195
Author
Organization
Abstract
Cryofixation immediately arrests all biochemical, physiological and dynamic processes underway in the sample in their present state, resulting in both excellent preservation of the specimen's ultrastructure and its antigenicity. Cryofixation involves extremely rapid cooling of specimens, creating an amorphous, or 'non-crystalline', state of water containing no detectable ice crystals, a process dependent on pressure, medium composition and temperature. Self-Pressurised Rapid Freezing (SPRF) employs plunge freezing of specimens in a sealed copper tube into a cryogen such as nitrogen slush (-210 degrees C),liquid nitrogen (-196 degrees C), ethane (-183 degrees C) or propane (-120 degrees C). In this study we have explored the use of SPRF with cooled acetone on dry ice (-80 degrees C) as the cryogen, a method named Drylce SPRF. Although with this relatively high temperature amorphous water cannot be formed, we have demonstrated that the ultrastructural and antigenicity results after Drylce SPRF on Caenorhabditis elegans are perfectly comparable with those achieved using High Pressure Freezing and SPRF. Thus, with sufficient pressure optimal results, with ice crystals below the resolution of transmission electron microscopy. can be achieved even at -78 degrees C. Furthermore, a huge advantage of Drylce SPRF over other techniques is its use of affordable, easily available and safe products.
Keywords
Agronomy and Crop Science, Ecology, Evolution, Behavior and Systematics, acetone, Caenorhabditis elegans, cellulose capillary, immunolocalisation, isochoric freezing, transmission electron microscopy, ultrastructure, ELECTRON-MICROSCOPY, ULTRASTRUCTURAL ANALYSIS, CRYOELECTRON MICROSCOPY, SPECIMEN PREPARATION, CELL-SUSPENSIONS, PRESERVATION, SUBSTITUTION, WATER, TREHALOSE, FIXATION

Downloads

  • Download
    (...).pdf
    • full text (Published version)
    • |
    • UGent only
    • |
    • PDF
    • |
    • 9.85 MB

Citation

Please use this url to cite or link to this publication:

MLA
Claeys, Myriam, et al. “Dry Ice Self-Pressurised Rapid Freezing (DryIce SPRF): All You Need to Cool for Cryofixation of Nematodes Is Dry Ice.” NEMATOLOGY, vol. 23, no. 10, 2021, pp. 1179–95, doi:10.1163/15685411-bja10104.
APA
Claeys, M., Yushin, V. V., & Bert, W. (2021). Dry Ice Self-Pressurised Rapid Freezing (DryIce SPRF): all you need to cool for cryofixation of nematodes is dry ice. NEMATOLOGY, 23(10), 1179–1195. https://doi.org/10.1163/15685411-bja10104
Chicago author-date
Claeys, Myriam, Vladimir V. Yushin, and Wim Bert. 2021. “Dry Ice Self-Pressurised Rapid Freezing (DryIce SPRF): All You Need to Cool for Cryofixation of Nematodes Is Dry Ice.” NEMATOLOGY 23 (10): 1179–95. https://doi.org/10.1163/15685411-bja10104.
Chicago author-date (all authors)
Claeys, Myriam, Vladimir V. Yushin, and Wim Bert. 2021. “Dry Ice Self-Pressurised Rapid Freezing (DryIce SPRF): All You Need to Cool for Cryofixation of Nematodes Is Dry Ice.” NEMATOLOGY 23 (10): 1179–1195. doi:10.1163/15685411-bja10104.
Vancouver
1.
Claeys M, Yushin VV, Bert W. Dry Ice Self-Pressurised Rapid Freezing (DryIce SPRF): all you need to cool for cryofixation of nematodes is dry ice. NEMATOLOGY. 2021;23(10):1179–95.
IEEE
[1]
M. Claeys, V. V. Yushin, and W. Bert, “Dry Ice Self-Pressurised Rapid Freezing (DryIce SPRF): all you need to cool for cryofixation of nematodes is dry ice,” NEMATOLOGY, vol. 23, no. 10, pp. 1179–1195, 2021.
@article{8771260,
  abstract     = {{Cryofixation immediately arrests all biochemical, physiological and dynamic processes underway in the sample in their present state, resulting in both excellent preservation of the specimen's ultrastructure and its antigenicity. Cryofixation involves extremely rapid cooling of specimens, creating an amorphous, or 'non-crystalline', state of water containing no detectable ice crystals, a process dependent on pressure, medium composition and temperature. Self-Pressurised Rapid Freezing (SPRF) employs plunge freezing of specimens in a sealed copper tube into a cryogen such as nitrogen slush (-210 degrees C),liquid nitrogen (-196 degrees C), ethane (-183 degrees C) or propane (-120 degrees C). In this study we have explored the use of SPRF with cooled acetone on dry ice (-80 degrees C) as the cryogen, a method named Drylce SPRF. Although with this relatively high temperature amorphous water cannot be formed, we have demonstrated that the ultrastructural and antigenicity results after Drylce SPRF on Caenorhabditis elegans are perfectly comparable with those achieved using High Pressure Freezing and SPRF. Thus, with sufficient pressure optimal results, with ice crystals below the resolution of transmission electron microscopy. can be achieved even at -78 degrees C. Furthermore, a huge advantage of Drylce SPRF over other techniques is its use of affordable, easily available and safe products.}},
  author       = {{Claeys, Myriam and Yushin, Vladimir V. and Bert, Wim}},
  issn         = {{1388-5545}},
  journal      = {{NEMATOLOGY}},
  keywords     = {{Agronomy and Crop Science,Ecology,Evolution,Behavior and Systematics,acetone,Caenorhabditis elegans,cellulose capillary,immunolocalisation,isochoric freezing,transmission electron microscopy,ultrastructure,ELECTRON-MICROSCOPY,ULTRASTRUCTURAL ANALYSIS,CRYOELECTRON MICROSCOPY,SPECIMEN PREPARATION,CELL-SUSPENSIONS,PRESERVATION,SUBSTITUTION,WATER,TREHALOSE,FIXATION}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{1179--1195}},
  title        = {{Dry Ice Self-Pressurised Rapid Freezing (DryIce SPRF): all you need to cool for cryofixation of nematodes is dry ice}},
  url          = {{http://doi.org/10.1163/15685411-bja10104}},
  volume       = {{23}},
  year         = {{2021}},
}
Altmetric
View in Altmetric
Web of Science
Times cited: