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Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes

Rupert Mayer (UGent) , Rein Verbeke (UGent) , Caroline Asselman (UGent) , Ilke Aernout (UGent) , Adillah Gul (UGent) , Denzel Eggermont (UGent) , Katie Boucher (UGent) , Fabien Henri Thery (UGent) , Teresa Maia (UGent) , Hans Demol (UGent) , et al.
(2022) NATURE COMMUNICATIONS. 13(1).
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Abstract
Listeria monocytogenes is a foodborne intracellular bacterial pathogen leading to human listeriosis. Despite a high mortality rate and increasing antibiotic resistance no clinically approved vaccine against Listeria is available. Attenuated Listeria strains offer protection and are tested as antitumor vaccine vectors, but would benefit from a better knowledge on immunodominant vector antigens. To identify novel antigens, we screen for Listeria peptides presented on the surface of infected human cell lines by mass spectrometry-based immunopeptidomics. In between more than 15,000 human self-peptides, we detect 68 Listeria immunopeptides from 42 different bacterial proteins, including several known antigens. Peptides presented on different cell lines are often derived from the same bacterial surface proteins, classifying these antigens as potential vaccine candidates. Encoding these highly presented antigens in lipid nanoparticle mRNA vaccine formulations results in specific CD8(+) T-cell responses and induces protection in vaccination challenge experiments in mice. Our results can serve as a starting point for the development of a clinical mRNA vaccine against Listeria and aid to improve attenuated Listeria vaccines and vectors, demonstrating the power of immunopeptidomics for next-generation bacterial vaccine development. Currently, no approved vaccines for Listeria monocytogenes are available. Here, the authors use immunopeptidomics to map bacterial peptides presented on infected cells and identify antigens that, as mRNA vaccine, provide protection in mice.

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MLA
Mayer, Rupert, et al. “Immunopeptidomics-Based Design of MRNA Vaccine Formulations against Listeria Monocytogenes.” NATURE COMMUNICATIONS, vol. 13, no. 1, 2022, doi:10.1038/s41467-022-33721-y.
APA
Mayer, R., Verbeke, R., Asselman, C., Aernout, I., Gul, A., Eggermont, D., … Impens, F. (2022). Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes. NATURE COMMUNICATIONS, 13(1). https://doi.org/10.1038/s41467-022-33721-y
Chicago author-date
Mayer, Rupert, Rein Verbeke, Caroline Asselman, Ilke Aernout, Adillah Gul, Denzel Eggermont, Katie Boucher, et al. 2022. “Immunopeptidomics-Based Design of MRNA Vaccine Formulations against Listeria Monocytogenes.” NATURE COMMUNICATIONS 13 (1). https://doi.org/10.1038/s41467-022-33721-y.
Chicago author-date (all authors)
Mayer, Rupert, Rein Verbeke, Caroline Asselman, Ilke Aernout, Adillah Gul, Denzel Eggermont, Katie Boucher, Fabien Henri Thery, Teresa Maia, Hans Demol, Ralf Gabriels, Lennart Martens, Christophe Bécavin, Stefaan De Smedt, Bart Vandekerckhove, Ine Lentacker, and Francis Impens. 2022. “Immunopeptidomics-Based Design of MRNA Vaccine Formulations against Listeria Monocytogenes.” NATURE COMMUNICATIONS 13 (1). doi:10.1038/s41467-022-33721-y.
Vancouver
1.
Mayer R, Verbeke R, Asselman C, Aernout I, Gul A, Eggermont D, et al. Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes. NATURE COMMUNICATIONS. 2022;13(1).
IEEE
[1]
R. Mayer et al., “Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes,” NATURE COMMUNICATIONS, vol. 13, no. 1, 2022.
@article{8770650,
  abstract     = {{Listeria monocytogenes is a foodborne intracellular bacterial pathogen leading to human listeriosis. Despite a high mortality rate and increasing antibiotic resistance no clinically approved vaccine against Listeria is available. Attenuated Listeria strains offer protection and are tested as antitumor vaccine vectors, but would benefit from a better knowledge on immunodominant vector antigens. To identify novel antigens, we screen for Listeria peptides presented on the surface of infected human cell lines by mass spectrometry-based immunopeptidomics. In between more than 15,000 human self-peptides, we detect 68 Listeria immunopeptides from 42 different bacterial proteins, including several known antigens. Peptides presented on different cell lines are often derived from the same bacterial surface proteins, classifying these antigens as potential vaccine candidates. Encoding these highly presented antigens in lipid nanoparticle mRNA vaccine formulations results in specific CD8(+) T-cell responses and induces protection in vaccination challenge experiments in mice. Our results can serve as a starting point for the development of a clinical mRNA vaccine against Listeria and aid to improve attenuated Listeria vaccines and vectors, demonstrating the power of immunopeptidomics for next-generation bacterial vaccine development.

Currently, no approved vaccines for Listeria monocytogenes are available. Here, the authors use immunopeptidomics to map bacterial peptides presented on infected cells and identify antigens that, as mRNA vaccine, provide protection in mice.}},
  articleno    = {{6075}},
  author       = {{Mayer, Rupert and Verbeke, Rein and Asselman, Caroline and Aernout, Ilke and Gul, Adillah and Eggermont, Denzel and Boucher, Katie and Thery, Fabien Henri and Maia, Teresa and Demol, Hans and Gabriels, Ralf and Martens, Lennart and Bécavin, Christophe and De Smedt, Stefaan and Vandekerckhove, Bart and Lentacker, Ine and Impens, Francis}},
  issn         = {{2041-1723}},
  journal      = {{NATURE COMMUNICATIONS}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{17}},
  title        = {{Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes}},
  url          = {{http://doi.org/10.1038/s41467-022-33721-y}},
  volume       = {{13}},
  year         = {{2022}},
}
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