The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residency
- Author
- Sigrid Wahlen, Filip Matthijssens (UGent) , Wouter Van Loocke (UGent) , Sylvie Taveirne (UGent) , Laura Kiekens, Eva Persyn, Els Van Ammel (UGent) , Zenzi De Vos (UGent) , Stijn De Munter (UGent) , Patrick Matthys, Filip Van Nieuwerburgh (UGent) , Tom Taghon (UGent) , Bart Vandekerckhove (UGent) , Pieter Van Vlierberghe (UGent) and Georges Leclercq (UGent)
- Organization
- Project
-
- Transcriptional regulation of differentiation of human natural killer and innate lymphoid cells.
- Exploring important milestones in the differentiation of human natural killer cells and innate lymphoid cells: from stem cell to effector.
- Transcriptional regulation of human natural killer cell and innate lymphoid cell differentiation
- The regulatory role of the transcription factors T-bet and Eomes in development and function of human NK cells and innate lymphoid cells
- HPC-UGent: the central High Performance Computing infrastructure of Ghent University
- Abstract
- Natural killer (NK) cells are innate lymphocytes that eliminate virus-infected and cancer cells by cytotoxicity and cytokine secretion. In addition to circulating NK cells, distinct tissue-resident NK subsets have been identified in various organs. Although transcription factors regulating NK cell development and function have been extensively studied in mice, the role of RUNX2 in these processes has not been investigated, neither in mice nor in human. Here, by manipulating RUNX2 expression with either knockdown or overexpression in human haematopoietic stem cell-based NK cell differentiation cultures, combined with transcriptomic and ChIP-sequencing analyses, we established that RUNX2 drives the generation of NK cells, possibly through induction of IL-2R beta expression in NK progenitor cells. Importantly, RUNX2 promotes tissue residency in human NK cells. Our findings have the potential to improve existing NK cell-based cancer therapies and can impact research fields beyond NK cell biology, since tissue-resident subsets have also been described in other lymphocyte subpopulations.
- Keywords
- General Immunology and Microbiology, General Biochemistry, Genetics and Molecular Biology, General Medicine, General Neuroscience
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8770253
- MLA
- Wahlen, Sigrid, et al. “The Transcription Factor RUNX2 Drives the Generation of Human NK Cells and Promotes Tissue Residency.” ELIFE, vol. 11, 2022, doi:10.7554/elife.80320.
- APA
- Wahlen, S., Matthijssens, F., Van Loocke, W., Taveirne, S., Kiekens, L., Persyn, E., … Leclercq, G. (2022). The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residency. ELIFE, 11. https://doi.org/10.7554/elife.80320
- Chicago author-date
- Wahlen, Sigrid, Filip Matthijssens, Wouter Van Loocke, Sylvie Taveirne, Laura Kiekens, Eva Persyn, Els Van Ammel, et al. 2022. “The Transcription Factor RUNX2 Drives the Generation of Human NK Cells and Promotes Tissue Residency.” ELIFE 11. https://doi.org/10.7554/elife.80320.
- Chicago author-date (all authors)
- Wahlen, Sigrid, Filip Matthijssens, Wouter Van Loocke, Sylvie Taveirne, Laura Kiekens, Eva Persyn, Els Van Ammel, Zenzi De Vos, Stijn De Munter, Patrick Matthys, Filip Van Nieuwerburgh, Tom Taghon, Bart Vandekerckhove, Pieter Van Vlierberghe, and Georges Leclercq. 2022. “The Transcription Factor RUNX2 Drives the Generation of Human NK Cells and Promotes Tissue Residency.” ELIFE 11. doi:10.7554/elife.80320.
- Vancouver
- 1.Wahlen S, Matthijssens F, Van Loocke W, Taveirne S, Kiekens L, Persyn E, et al. The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residency. ELIFE. 2022;11.
- IEEE
- [1]S. Wahlen et al., “The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residency,” ELIFE, vol. 11, 2022.
@article{8770253,
abstract = {{Natural killer (NK) cells are innate lymphocytes that eliminate virus-infected and cancer cells by cytotoxicity and cytokine secretion. In addition to circulating NK cells, distinct tissue-resident NK subsets have been identified in various organs. Although transcription factors regulating NK cell development and function have been extensively studied in mice, the role of RUNX2 in these processes has not been investigated, neither in mice nor in human. Here, by manipulating RUNX2 expression with either knockdown or overexpression in human haematopoietic stem cell-based NK cell differentiation cultures, combined with transcriptomic and ChIP-sequencing analyses, we established that RUNX2 drives the generation of NK cells, possibly through induction of IL-2R beta expression in NK progenitor cells. Importantly, RUNX2 promotes tissue residency in human NK cells. Our findings have the potential to improve existing NK cell-based cancer therapies and can impact research fields beyond NK cell biology, since tissue-resident subsets have also been described in other lymphocyte subpopulations.}},
articleno = {{e80320}},
author = {{Wahlen, Sigrid and Matthijssens, Filip and Van Loocke, Wouter and Taveirne, Sylvie and Kiekens, Laura and Persyn, Eva and Van Ammel, Els and De Vos, Zenzi and De Munter, Stijn and Matthys, Patrick and Van Nieuwerburgh, Filip and Taghon, Tom and Vandekerckhove, Bart and Van Vlierberghe, Pieter and Leclercq, Georges}},
issn = {{2050-084X}},
journal = {{ELIFE}},
keywords = {{General Immunology and Microbiology,General Biochemistry,Genetics and Molecular Biology,General Medicine,General Neuroscience}},
language = {{eng}},
pages = {{25}},
title = {{The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residency}},
url = {{http://doi.org/10.7554/elife.80320}},
volume = {{11}},
year = {{2022}},
}
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