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Photodisruption of the inner limiting membrane : exploring ICG loaded nanoparticles as photosensitizers

Kaat De Clerck (UGent) , Geraldine Accou (UGent) , Félix Sauvage (UGent) , Kevin Braeckmans (UGent) , Stefaan De Smedt (UGent) , Katrien Remaut (UGent) and Karen Peynshaert (UGent)
(2022) PHARMACEUTICS. 14(8).
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Abstract
The inner limiting membrane (ILM) represents a major bottleneck hampering efficient drug delivery to the retina after intravitreal injection. To overcome this barrier, we intend to perforate the ILM by use of a light-based approach which relies on the creation of vapor nanobubbles (VNBs) when irradiating photosensitizers with high intensity laser pulses. Upon collapse of these VNBs, mechanical effects can disrupt biological structures. As a photosensitizer, we explore indocyanine green (ICG) loaded nanoparticles (NPs) specifically designed for our application. In light of this, ICG liposomes and PLGA ICG NPs were characterized in terms of physicochemical properties, ICG incorporation and VNB formation. ICG liposomes were found to encapsulate significantly higher amounts of ICG compared to PLGA ICG NPs which is reflected in their VNB creating capacity. Since only ICG liposomes were able to induce VNB generation, this class of NPs was further investigated on retinal explants. Here, application of ICG liposomes followed by laser treatment resulted in subtle disruption effects at the ILM where zones of fully ablated ILM were alternated by intact regions. As the interaction between the ICG liposomes and ILM might be insufficient, active targeting strategies or other NP designs might improve the concept to a further extent.
Keywords
INDOCYANINE-GREEN, FLUORESCENCE PROPERTIES, PARTICLE-SIZE, TRANSDUCTION, LIPOSOMES, BARRIERS, DELIVERY, FUTURE, retinal drug delivery, inner limiting membrane, photodisruption, vapor, nanobubbles, indocyanine green, nanotechnology, pulsed laser

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Citation

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MLA
De Clerck, Kaat, et al. “Photodisruption of the Inner Limiting Membrane : Exploring ICG Loaded Nanoparticles as Photosensitizers.” PHARMACEUTICS, vol. 14, no. 8, 2022, doi:10.3390/pharmaceutics14081716.
APA
De Clerck, K., Accou, G., Sauvage, F., Braeckmans, K., De Smedt, S., Remaut, K., & Peynshaert, K. (2022). Photodisruption of the inner limiting membrane : exploring ICG loaded nanoparticles as photosensitizers. PHARMACEUTICS, 14(8). https://doi.org/10.3390/pharmaceutics14081716
Chicago author-date
De Clerck, Kaat, Geraldine Accou, Félix Sauvage, Kevin Braeckmans, Stefaan De Smedt, Katrien Remaut, and Karen Peynshaert. 2022. “Photodisruption of the Inner Limiting Membrane : Exploring ICG Loaded Nanoparticles as Photosensitizers.” PHARMACEUTICS 14 (8). https://doi.org/10.3390/pharmaceutics14081716.
Chicago author-date (all authors)
De Clerck, Kaat, Geraldine Accou, Félix Sauvage, Kevin Braeckmans, Stefaan De Smedt, Katrien Remaut, and Karen Peynshaert. 2022. “Photodisruption of the Inner Limiting Membrane : Exploring ICG Loaded Nanoparticles as Photosensitizers.” PHARMACEUTICS 14 (8). doi:10.3390/pharmaceutics14081716.
Vancouver
1.
De Clerck K, Accou G, Sauvage F, Braeckmans K, De Smedt S, Remaut K, et al. Photodisruption of the inner limiting membrane : exploring ICG loaded nanoparticles as photosensitizers. PHARMACEUTICS. 2022;14(8).
IEEE
[1]
K. De Clerck et al., “Photodisruption of the inner limiting membrane : exploring ICG loaded nanoparticles as photosensitizers,” PHARMACEUTICS, vol. 14, no. 8, 2022.
@article{8768551,
  abstract     = {{The inner limiting membrane (ILM) represents a major bottleneck hampering efficient drug delivery to the retina after intravitreal injection. To overcome this barrier, we intend to perforate the ILM by use of a light-based approach which relies on the creation of vapor nanobubbles (VNBs) when irradiating photosensitizers with high intensity laser pulses. Upon collapse of these VNBs, mechanical effects can disrupt biological structures. As a photosensitizer, we explore indocyanine green (ICG) loaded nanoparticles (NPs) specifically designed for our application. In light of this, ICG liposomes and PLGA ICG NPs were characterized in terms of physicochemical properties, ICG incorporation and VNB formation. ICG liposomes were found to encapsulate significantly higher amounts of ICG compared to PLGA ICG NPs which is reflected in their VNB creating capacity. Since only ICG liposomes were able to induce VNB generation, this class of NPs was further investigated on retinal explants. Here, application of ICG liposomes followed by laser treatment resulted in subtle disruption effects at the ILM where zones of fully ablated ILM were alternated by intact regions. As the interaction between the ICG liposomes and ILM might be insufficient, active targeting strategies or other NP designs might improve the concept to a further extent.}},
  articleno    = {{1716}},
  author       = {{De Clerck, Kaat and Accou, Geraldine and Sauvage, Félix and Braeckmans, Kevin and De Smedt, Stefaan and Remaut, Katrien and Peynshaert, Karen}},
  issn         = {{1999-4923}},
  journal      = {{PHARMACEUTICS}},
  keywords     = {{INDOCYANINE-GREEN,FLUORESCENCE PROPERTIES,PARTICLE-SIZE,TRANSDUCTION,LIPOSOMES,BARRIERS,DELIVERY,FUTURE,retinal drug delivery,inner limiting membrane,photodisruption,vapor,nanobubbles,indocyanine green,nanotechnology,pulsed laser}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{17}},
  title        = {{Photodisruption of the inner limiting membrane : exploring ICG loaded nanoparticles as photosensitizers}},
  url          = {{http://doi.org/10.3390/pharmaceutics14081716}},
  volume       = {{14}},
  year         = {{2022}},
}
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