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Phenotype and RNA-seq-Based transcriptome profiling of Staphylococcus aureus biofilms in response to tea tree oil

Xingchen Zhao, Zonghui Liu, Zuojia Liu, Rizeng Meng, Ce Shi, Xiangrong Chen, Xiujuan Bu and Na Guo
(2018) MICROBIAL PATHOGENESIS. 123. p.304-313
Author
Organization
Abstract
Staphylococcus aureus (S. aureus) is a Gram-positive bacterium that causes a wide range of diseases, including food poisoning. Tea tree oil (TTO), an essential oil distilled from Melaleuca alternifolia, is well-known for its antibacterial activities. TTO effectively inhibited all 19 tested strains of S. aureus biofilm and planktonic cells. Phenotype analyses of S. aureus biofilm cells exposed to TTO were performed by biofilm adhesion assays, eDNA detection and PIA release. RNA sequencing (RNA-seq) was used in our study to elucidate the mechanism of TTO as a potential antibacterial agent to evaluate differentially expressed genes (DEGs) and the functional network in S. aureus ATCC 29213 biofilms. TTO significantly changed (greater than a 2- or less than a 2-fold change) the expression of 304 genes in S. aureus contained in biofilms. The levels of genes related to the glycine, serine and threonine metabolism pathway, purine metabolism pathway, pyrimidine metabolism pathway and amino acid biosynthesis pathway were dramatically changed in the biofilm exposed to no. Furthermore, the expression changes identified by RNA-seq analysis were verified by real-time RT-PCR. To the best of our knowledge, this research is the first study to report the phenotype and expression profiles of S. aureus in biofilms exposed to TTO.
Keywords
Staphylococcus aureus, Tea tree oil, Biofilm, RNA sequencing, ESCHERICHIA-COLI, ANTIBIOFILM ACTIVITY, GENE-EXPRESSION, EPIDERMIDIS, COMPONENTS, GROWTH, INFECTIONS, RESISTANCE, CELLS, SITES

Citation

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MLA
Zhao, Xingchen, et al. “Phenotype and RNA-Seq-Based Transcriptome Profiling of Staphylococcus Aureus Biofilms in Response to Tea Tree Oil.” MICROBIAL PATHOGENESIS, vol. 123, 2018, pp. 304–13, doi:10.1016/j.micpath.2018.07.027.
APA
Zhao, X., Liu, Z., Liu, Z., Meng, R., Shi, C., Chen, X., … Guo, N. (2018). Phenotype and RNA-seq-Based transcriptome profiling of Staphylococcus aureus biofilms in response to tea tree oil. MICROBIAL PATHOGENESIS, 123, 304–313. https://doi.org/10.1016/j.micpath.2018.07.027
Chicago author-date
Zhao, Xingchen, Zonghui Liu, Zuojia Liu, Rizeng Meng, Ce Shi, Xiangrong Chen, Xiujuan Bu, and Na Guo. 2018. “Phenotype and RNA-Seq-Based Transcriptome Profiling of Staphylococcus Aureus Biofilms in Response to Tea Tree Oil.” MICROBIAL PATHOGENESIS 123: 304–13. https://doi.org/10.1016/j.micpath.2018.07.027.
Chicago author-date (all authors)
Zhao, Xingchen, Zonghui Liu, Zuojia Liu, Rizeng Meng, Ce Shi, Xiangrong Chen, Xiujuan Bu, and Na Guo. 2018. “Phenotype and RNA-Seq-Based Transcriptome Profiling of Staphylococcus Aureus Biofilms in Response to Tea Tree Oil.” MICROBIAL PATHOGENESIS 123: 304–313. doi:10.1016/j.micpath.2018.07.027.
Vancouver
1.
Zhao X, Liu Z, Liu Z, Meng R, Shi C, Chen X, et al. Phenotype and RNA-seq-Based transcriptome profiling of Staphylococcus aureus biofilms in response to tea tree oil. MICROBIAL PATHOGENESIS. 2018;123:304–13.
IEEE
[1]
X. Zhao et al., “Phenotype and RNA-seq-Based transcriptome profiling of Staphylococcus aureus biofilms in response to tea tree oil,” MICROBIAL PATHOGENESIS, vol. 123, pp. 304–313, 2018.
@article{8768075,
  abstract     = {{Staphylococcus aureus (S. aureus) is a Gram-positive bacterium that causes a wide range of diseases, including food poisoning. Tea tree oil (TTO), an essential oil distilled from Melaleuca alternifolia, is well-known for its antibacterial activities. TTO effectively inhibited all 19 tested strains of S. aureus biofilm and planktonic cells. Phenotype analyses of S. aureus biofilm cells exposed to TTO were performed by biofilm adhesion assays, eDNA detection and PIA release. RNA sequencing (RNA-seq) was used in our study to elucidate the mechanism of TTO as a potential antibacterial agent to evaluate differentially expressed genes (DEGs) and the functional network in S. aureus ATCC 29213 biofilms. TTO significantly changed (greater than a 2- or less than a 2-fold change) the expression of 304 genes in S. aureus contained in biofilms. The levels of genes related to the glycine, serine and threonine metabolism pathway, purine metabolism pathway, pyrimidine metabolism pathway and amino acid biosynthesis pathway were dramatically changed in the biofilm exposed to no. Furthermore, the expression changes identified by RNA-seq analysis were verified by real-time RT-PCR. To the best of our knowledge, this research is the first study to report the phenotype and expression profiles of S. aureus in biofilms exposed to TTO.}},
  author       = {{Zhao, Xingchen and Liu, Zonghui and Liu, Zuojia and Meng, Rizeng and Shi, Ce and Chen, Xiangrong and Bu, Xiujuan and Guo, Na}},
  issn         = {{0882-4010}},
  journal      = {{MICROBIAL PATHOGENESIS}},
  keywords     = {{Staphylococcus aureus,Tea tree oil,Biofilm,RNA sequencing,ESCHERICHIA-COLI,ANTIBIOFILM ACTIVITY,GENE-EXPRESSION,EPIDERMIDIS,COMPONENTS,GROWTH,INFECTIONS,RESISTANCE,CELLS,SITES}},
  language     = {{eng}},
  pages        = {{304--313}},
  title        = {{Phenotype and RNA-seq-Based transcriptome profiling of Staphylococcus aureus biofilms in response to tea tree oil}},
  url          = {{http://doi.org/10.1016/j.micpath.2018.07.027}},
  volume       = {{123}},
  year         = {{2018}},
}
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