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Balance between the cell viability and death in 3D

Angela Debruyne (UGent) , Irina Okkelman (UGent) and Ruslan Dmitriev (UGent)
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Abstract
Cell death is a phenomenon, frequently perceived as an absolute event for cell, tissue and the organ. However, the rising popularity and complexity of such 3D multicellular 'tissue building blocks' as heterocellular spheroids, organoids, and 'assembloids' prompts to revise the definition and quantification of cell viability and death. It raises several questions on the overall viability of all the cells within 3D volume and on choosing the appropriate, continuous, and non-destructive viability assay enabling for a single-cell analysis. In this review, we look at cell viability and cell death modalities with attention to the intrinsic features of such 3D models as spheroids, organoids, and bioprints. Furthermore, we look at emerging and promising methodologies, which can help define and understand the balance between cell viability and death in dynamic and complex 3D environments. We conclude that the recent innovations in biofabrication, biosensor probe development, and fluorescence microscopy can help answer these questions.
Keywords
Cell Biology, Developmental Biology, Organoids, Multicellular spheroids, Microscopy, Cell viability, Cell death, Bioprint

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MLA
Debruyne, Angela, et al. “Balance between the Cell Viability and Death in 3D.” SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, vol. 144, 2023, pp. 55–66, doi:10.1016/j.semcdb.2022.09.005.
APA
Debruyne, A., Okkelman, I., & Dmitriev, R. (2023). Balance between the cell viability and death in 3D. SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 144, 55–66. https://doi.org/10.1016/j.semcdb.2022.09.005
Chicago author-date
Debruyne, Angela, Irina Okkelman, and Ruslan Dmitriev. 2023. “Balance between the Cell Viability and Death in 3D.” SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY 144: 55–66. https://doi.org/10.1016/j.semcdb.2022.09.005.
Chicago author-date (all authors)
Debruyne, Angela, Irina Okkelman, and Ruslan Dmitriev. 2023. “Balance between the Cell Viability and Death in 3D.” SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY 144: 55–66. doi:10.1016/j.semcdb.2022.09.005.
Vancouver
1.
Debruyne A, Okkelman I, Dmitriev R. Balance between the cell viability and death in 3D. SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY. 2023;144:55–66.
IEEE
[1]
A. Debruyne, I. Okkelman, and R. Dmitriev, “Balance between the cell viability and death in 3D,” SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, vol. 144, pp. 55–66, 2023.
@article{8766664,
  abstract     = {{Cell death is a phenomenon, frequently perceived as an absolute event for cell, tissue and the organ. However, the rising popularity and complexity of such 3D multicellular 'tissue building blocks' as heterocellular spheroids, organoids, and 'assembloids' prompts to revise the definition and quantification of cell viability and death. It raises several questions on the overall viability of all the cells within 3D volume and on choosing the appropriate, continuous, and non-destructive viability assay enabling for a single-cell analysis. In this review, we look at cell viability and cell death modalities with attention to the intrinsic features of such 3D models as spheroids, organoids, and bioprints. Furthermore, we look at emerging and promising methodologies, which can help define and understand the balance between cell viability and death in dynamic and complex 3D environments. We conclude that the recent innovations in biofabrication, biosensor probe development, and fluorescence microscopy can help answer these questions.}},
  author       = {{Debruyne, Angela and Okkelman, Irina and Dmitriev, Ruslan}},
  issn         = {{1084-9521}},
  journal      = {{SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY}},
  keywords     = {{Cell Biology,Developmental Biology,Organoids,Multicellular spheroids,Microscopy,Cell viability,Cell death,Bioprint}},
  language     = {{eng}},
  pages        = {{55--66}},
  title        = {{Balance between the cell viability and death in 3D}},
  url          = {{http://doi.org/10.1016/j.semcdb.2022.09.005}},
  volume       = {{144}},
  year         = {{2023}},
}

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