Effect of pectin on anti-diabetic and anti-oxidant properties of fermented milk by lactic acid bacteria isolated from traditional Iranian dairy products
- Author
- Faezeh Shirkhan, Saeed Mirdamadi, Mahta Mirzaei (UGent) , Behrouz Akbari-Adergani and Nikoo Nasoohi
- Organization
- Abstract
- Volume 19, Issue 123 (2022) FSCT 2022, 19(123): 55-67 | Back to browse issues page 10.52547/fsct.19.123.55 20.1001.1.20088787.1401.19.123.20.5 XML Persian Abstract Print Download citation: BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks Send citation to: Mendeley Zotero RefWorks shirkhan F, Mirdamadi S, Mahta Mirzaei M, Akbari-Adergani B, Nasoohi N. Effect of Pectin on Anti-diabetic and Anti-oxidant Properties of Fermented Milk by Lactic Acid Bacteria Isolated from Traditional Iranian Dairy Products. FSCT. 2022; 19 (123) :55-67 URL: http://fsct.modares.ac.ir/article-7-57521-en.html Effect of Pectin on Anti-diabetic and Anti-oxidant Properties of Fermented Milk by Lactic Acid Bacteria Isolated from Traditional Iranian Dairy Products Faezeh Shirkhan1, Saeed Mirdamadi2, Mahta Mahta Mirzaei3, Behrouz Akbari-Adergani4, Nikoo Nasoohi5 1- Department of Food Science and Technology, Faculty of Pharmacy, Tehran Medical Sience, Islamic Azad Univercity,Tehran, Iran 2- Professor, Department of Biotechnology, Iranian Research Organization for Science & Technology (IROST), Tehran, Iran , Mirdamadi@irost.ir 3- Assistant Professor, Department of Food Science and Technology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran 4- Professor of Food and Drug Laboratory Research Center, Food and Drug administration, Ministry of Health and Medical Education 5- Assistant Professor, Department of Biochemistry and Biophysics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran Abstract: (579 Views) Abstract Nowadays, Polysaccharides are used to improve the nutritional and physicochemical properties of dairy products. The identification of natural compounds such as polysaccharides with antidiabetic and antioxidant properties has become important due to the relationship between diabetes and oxidative stress. Therefore, the present study aimed to evaluate the influence of pectin on the antidiabetic and antioxidant activity of milk fermented by lactic acid bacteria (LAB) isolated from traditional Iranian dairy products. Pectin (1%) was added to the samples following milk fermentation by Lactobacillus helveticus and Lactobacillus para-casei strains, and antidiabetic activity was assessed by considering the inhibitory effects on α-amylase and α-glucosidase. The antioxidant activity was determined by evaluating inhibition of 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and hydroxyl radicals. Samples included pectin solution (0.5-3.5 mg/ml and 0.5-10 mg/ml for evaluation of anti-diabetic and antioxidant activity, respectively), whey of fermented and non-fermented milk with (1%) and without pectin. The results indicated the role of pectin on inhibition of α-glucosidase enzyme activity (IC50=2.38 mg/ml), as well as scavenging the DPPH (44.57%), ABTS (28.7%), and Hydroxyl (38.85%) radicals (P<0.05) a concentration of 10 mg/ml. Pectin added to the whey of non-fermented milk sample boosted antioxidant properties and the maximum rate of free radical scavenging activity (35%) was obtained for DPPH radicals. Furthermore, adding pectin to the whey of milk fermented with Lactobacillus helveticus strain improved the activity of the product on inhibition of α-glucosidase enzyme (29%), scavenging of DPPH (41.26 %), ABTS (2.5%), and hydroxyl radicals (7.64%) (P<0.05). The results indicated the potential of pectin to be used in the formulation of beneficial food products due to its ability to improve the antioxidant and anti-diabetic properties of fermented milk products.
- Keywords
- Type 2 diabetes, Pectin, Lactic Acid Bacteria, Polysaccharide, Antioxidant activity, Functional product
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8766039
- MLA
- Shirkhan, Faezeh, et al. “Effect of Pectin on Anti-Diabetic and Anti-Oxidant Properties of Fermented Milk by Lactic Acid Bacteria Isolated from Traditional Iranian Dairy Products.” JOURNAL OF FOOD SCIENCE AND TECHNOLOGY (IRAN), vol. 19, no. 123, 2022, pp. 55–67, doi:10.52547/fsct.19.123.55.
- APA
- Shirkhan, F., Mirdamadi, S., Mirzaei, M., Akbari-Adergani, B., & Nasoohi, N. (2022). Effect of pectin on anti-diabetic and anti-oxidant properties of fermented milk by lactic acid bacteria isolated from traditional Iranian dairy products. JOURNAL OF FOOD SCIENCE AND TECHNOLOGY (IRAN), 19(123), 55–67. https://doi.org/10.52547/fsct.19.123.55
- Chicago author-date
- Shirkhan, Faezeh, Saeed Mirdamadi, Mahta Mirzaei, Behrouz Akbari-Adergani, and Nikoo Nasoohi. 2022. “Effect of Pectin on Anti-Diabetic and Anti-Oxidant Properties of Fermented Milk by Lactic Acid Bacteria Isolated from Traditional Iranian Dairy Products.” JOURNAL OF FOOD SCIENCE AND TECHNOLOGY (IRAN) 19 (123): 55–67. https://doi.org/10.52547/fsct.19.123.55.
- Chicago author-date (all authors)
- Shirkhan, Faezeh, Saeed Mirdamadi, Mahta Mirzaei, Behrouz Akbari-Adergani, and Nikoo Nasoohi. 2022. “Effect of Pectin on Anti-Diabetic and Anti-Oxidant Properties of Fermented Milk by Lactic Acid Bacteria Isolated from Traditional Iranian Dairy Products.” JOURNAL OF FOOD SCIENCE AND TECHNOLOGY (IRAN) 19 (123): 55–67. doi:10.52547/fsct.19.123.55.
- Vancouver
- 1.Shirkhan F, Mirdamadi S, Mirzaei M, Akbari-Adergani B, Nasoohi N. Effect of pectin on anti-diabetic and anti-oxidant properties of fermented milk by lactic acid bacteria isolated from traditional Iranian dairy products. JOURNAL OF FOOD SCIENCE AND TECHNOLOGY (IRAN). 2022;19(123):55–67.
- IEEE
- [1]F. Shirkhan, S. Mirdamadi, M. Mirzaei, B. Akbari-Adergani, and N. Nasoohi, “Effect of pectin on anti-diabetic and anti-oxidant properties of fermented milk by lactic acid bacteria isolated from traditional Iranian dairy products,” JOURNAL OF FOOD SCIENCE AND TECHNOLOGY (IRAN), vol. 19, no. 123, pp. 55–67, 2022.
@article{8766039,
abstract = {{Volume 19, Issue 123 (2022) FSCT 2022, 19(123): 55-67 | Back to browse issues page
10.52547/fsct.19.123.55
20.1001.1.20088787.1401.19.123.20.5
XML Persian Abstract Print
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Mendeley
Zotero
RefWorks
shirkhan F, Mirdamadi S, Mahta Mirzaei M, Akbari-Adergani B, Nasoohi N. Effect of Pectin on Anti-diabetic and Anti-oxidant Properties of Fermented Milk by Lactic Acid Bacteria Isolated from Traditional Iranian Dairy Products. FSCT. 2022; 19 (123) :55-67
URL: http://fsct.modares.ac.ir/article-7-57521-en.html
Effect of Pectin on Anti-diabetic and Anti-oxidant Properties of Fermented Milk by Lactic Acid Bacteria Isolated from Traditional Iranian Dairy Products
Faezeh Shirkhan1, Saeed Mirdamadi2, Mahta Mahta Mirzaei3, Behrouz Akbari-Adergani4, Nikoo Nasoohi5
1- Department of Food Science and Technology, Faculty of Pharmacy, Tehran Medical Sience, Islamic Azad Univercity,Tehran, Iran
2- Professor, Department of Biotechnology, Iranian Research Organization for Science & Technology (IROST), Tehran, Iran , Mirdamadi@irost.ir
3- Assistant Professor, Department of Food Science and Technology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
4- Professor of Food and Drug Laboratory Research Center, Food and Drug administration, Ministry of Health and Medical Education
5- Assistant Professor, Department of Biochemistry and Biophysics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Abstract: (579 Views)
Abstract
Nowadays, Polysaccharides are used to improve the nutritional and physicochemical properties of dairy products. The identification of natural compounds such as polysaccharides with antidiabetic and antioxidant properties has become important due to the relationship between diabetes and oxidative stress. Therefore, the present study aimed to evaluate the influence of pectin on the antidiabetic and antioxidant activity of milk fermented by lactic acid bacteria (LAB) isolated from traditional Iranian dairy products. Pectin (1%) was added to the samples following milk fermentation by Lactobacillus helveticus and Lactobacillus para-casei strains, and antidiabetic activity was assessed by considering the inhibitory effects on α-amylase and α-glucosidase. The antioxidant activity was determined by evaluating inhibition of 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and hydroxyl radicals. Samples included pectin solution (0.5-3.5 mg/ml and 0.5-10 mg/ml for evaluation of anti-diabetic and antioxidant activity, respectively), whey of fermented and non-fermented milk with (1%) and without pectin. The results indicated the role of pectin on inhibition of α-glucosidase enzyme activity (IC50=2.38 mg/ml), as well as scavenging the DPPH (44.57%), ABTS (28.7%), and Hydroxyl (38.85%) radicals (P<0.05) a concentration of 10 mg/ml. Pectin added to the whey of non-fermented milk sample boosted antioxidant properties and the maximum rate of free radical scavenging activity (35%) was obtained for DPPH radicals. Furthermore, adding pectin to the whey of milk fermented with Lactobacillus helveticus strain improved the activity of the product on inhibition of α-glucosidase enzyme (29%), scavenging of DPPH (41.26 %), ABTS (2.5%), and hydroxyl radicals (7.64%) (P<0.05). The results indicated the potential of pectin to be used in the formulation of beneficial food products due to its ability to improve the antioxidant and anti-diabetic properties of fermented milk products.}},
author = {{Shirkhan, Faezeh and Mirdamadi, Saeed and Mirzaei, Mahta and Akbari-Adergani, Behrouz and Nasoohi, Nikoo}},
issn = {{2008-8787}},
journal = {{JOURNAL OF FOOD SCIENCE AND TECHNOLOGY (IRAN)}},
keywords = {{Type 2 diabetes,Pectin,Lactic Acid Bacteria,Polysaccharide,Antioxidant activity,Functional product}},
language = {{per}},
number = {{123}},
pages = {{55--67}},
title = {{Effect of pectin on anti-diabetic and anti-oxidant properties of fermented milk by lactic acid bacteria isolated from traditional Iranian dairy products}},
url = {{http://doi.org/10.52547/fsct.19.123.55}},
volume = {{19}},
year = {{2022}},
}
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