Synthesis, in vitro and cellular antioxidant activity evaluation of novel peptides derived from Saccharomyces cerevisiae protein hydrolysate : structure-function relationship
- Author
- Mahta Mirzaei (UGent) , Saeed Mirdamadi, Maliheh Safavi, Davood Zare, Mahnaz Hadizadeh and Mahnaz Mazaheri Asadi
- Organization
- Abstract
- The relationship between structure and function of primary antioxidant peptide, YR-10 (YGKPVAVPAR) was considered by synthesizing three analogues including YHR-10 (YGKHVAVHAR), GA-8 (GKPVAVPA) and PAR-3 (PAR). Antioxidant activity was determined through in vitro and cellular assays. Substitution of Pro with His in the structure of YR-10 led to significant (P < 0.05) higher ABTS radical scavenging and ferric reducing activity. Following in silico simulated gastrointestinal digestion, Tyr and Arg were omitted, respectively, from N and C-terminal positions and resulted in decreasing DPPH, ABTS radical scavenging, and ferric reducing activities. PAR-3 showed the best inhibitory activity on linoleic acid oxidation. Pretreatment of Caco-2 cells with YR-10, YHR-10, and GA-8 (1000 mu M) before exposure to H2O2 (160 mu M) resulted in 34.10%, 39.66% and 29.159% reduction in malondialdehyde and 53.52%, 17.02% and 24.71% reduction in protein carbonyl levels. The peptide pretreatment reduced catalase level in cells and PAR-3 exhibited the most protective effects on the viability of cells exposed to oxidative stress.
- Keywords
- Synthetic peptides, Structure-function, Antioxidant activity, Caco-2 cells, ANTICANCER PEPTIDES, OXIDATIVE DAMAGE, IDENTIFICATION, PURIFICATION, MUSCLE, PROLIFERATION, CAPACITY, ASSAY, POWER, FRAP
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8766031
- MLA
- Mirzaei, Mahta, et al. “Synthesis, in Vitro and Cellular Antioxidant Activity Evaluation of Novel Peptides Derived from Saccharomyces Cerevisiae Protein Hydrolysate : Structure-Function Relationship.” AMINO ACIDS, vol. 51, no. 8, 2019, pp. 1167–75, doi:10.1007/s00726-019-02752-z.
- APA
- Mirzaei, M., Mirdamadi, S., Safavi, M., Zare, D., Hadizadeh, M., & Asadi, M. M. (2019). Synthesis, in vitro and cellular antioxidant activity evaluation of novel peptides derived from Saccharomyces cerevisiae protein hydrolysate : structure-function relationship. AMINO ACIDS, 51(8), 1167–1175. https://doi.org/10.1007/s00726-019-02752-z
- Chicago author-date
- Mirzaei, Mahta, Saeed Mirdamadi, Maliheh Safavi, Davood Zare, Mahnaz Hadizadeh, and Mahnaz Mazaheri Asadi. 2019. “Synthesis, in Vitro and Cellular Antioxidant Activity Evaluation of Novel Peptides Derived from Saccharomyces Cerevisiae Protein Hydrolysate : Structure-Function Relationship.” AMINO ACIDS 51 (8): 1167–75. https://doi.org/10.1007/s00726-019-02752-z.
- Chicago author-date (all authors)
- Mirzaei, Mahta, Saeed Mirdamadi, Maliheh Safavi, Davood Zare, Mahnaz Hadizadeh, and Mahnaz Mazaheri Asadi. 2019. “Synthesis, in Vitro and Cellular Antioxidant Activity Evaluation of Novel Peptides Derived from Saccharomyces Cerevisiae Protein Hydrolysate : Structure-Function Relationship.” AMINO ACIDS 51 (8): 1167–1175. doi:10.1007/s00726-019-02752-z.
- Vancouver
- 1.Mirzaei M, Mirdamadi S, Safavi M, Zare D, Hadizadeh M, Asadi MM. Synthesis, in vitro and cellular antioxidant activity evaluation of novel peptides derived from Saccharomyces cerevisiae protein hydrolysate : structure-function relationship. AMINO ACIDS. 2019;51(8):1167–75.
- IEEE
- [1]M. Mirzaei, S. Mirdamadi, M. Safavi, D. Zare, M. Hadizadeh, and M. M. Asadi, “Synthesis, in vitro and cellular antioxidant activity evaluation of novel peptides derived from Saccharomyces cerevisiae protein hydrolysate : structure-function relationship,” AMINO ACIDS, vol. 51, no. 8, pp. 1167–1175, 2019.
@article{8766031,
abstract = {{The relationship between structure and function of primary antioxidant peptide, YR-10 (YGKPVAVPAR) was considered by synthesizing three analogues including YHR-10 (YGKHVAVHAR), GA-8 (GKPVAVPA) and PAR-3 (PAR). Antioxidant activity was determined through in vitro and cellular assays. Substitution of Pro with His in the structure of YR-10 led to significant (P < 0.05) higher ABTS radical scavenging and ferric reducing activity. Following in silico simulated gastrointestinal digestion, Tyr and Arg were omitted, respectively, from N and C-terminal positions and resulted in decreasing DPPH, ABTS radical scavenging, and ferric reducing activities. PAR-3 showed the best inhibitory activity on linoleic acid oxidation. Pretreatment of Caco-2 cells with YR-10, YHR-10, and GA-8 (1000 mu M) before exposure to H2O2 (160 mu M) resulted in 34.10%, 39.66% and 29.159% reduction in malondialdehyde and 53.52%, 17.02% and 24.71% reduction in protein carbonyl levels. The peptide pretreatment reduced catalase level in cells and PAR-3 exhibited the most protective effects on the viability of cells exposed to oxidative stress.}},
author = {{Mirzaei, Mahta and Mirdamadi, Saeed and Safavi, Maliheh and Zare, Davood and Hadizadeh, Mahnaz and Asadi, Mahnaz Mazaheri}},
issn = {{0939-4451}},
journal = {{AMINO ACIDS}},
keywords = {{Synthetic peptides,Structure-function,Antioxidant activity,Caco-2 cells,ANTICANCER PEPTIDES,OXIDATIVE DAMAGE,IDENTIFICATION,PURIFICATION,MUSCLE,PROLIFERATION,CAPACITY,ASSAY,POWER,FRAP}},
language = {{eng}},
number = {{8}},
pages = {{1167--1175}},
title = {{Synthesis, in vitro and cellular antioxidant activity evaluation of novel peptides derived from Saccharomyces cerevisiae protein hydrolysate : structure-function relationship}},
url = {{http://doi.org/10.1007/s00726-019-02752-z}},
volume = {{51}},
year = {{2019}},
}
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: