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The quorum sensing peptide EntF* promotes colorectal cancer metastasis in mice: a new factor in the host-microbiome interaction

Author
Abstract
<jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:p>Colorectal cancer, one of the most common malignancies worldwide, is associated with a high mortality rate, mainly caused by metastasis. Comparative metagenome-wide association analyses of healthy individuals and cancer patients suggest a role for the human intestinal microbiota in tumor progression. However, the microbial molecules involved in host-microbe communication are largely unknown, with current studies mainly focusing on short-chain fatty acids and amino acid metabolites as potential mediators. Quorum sensing peptides are not yet considered in this context since their presence in vivo and their ability to affect host cells have not been reported so far.</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>Here, we show that EntF*, a metabolite of the quorum sensing peptide EntF produced by <jats:italic>Enterococcus faecium</jats:italic>, is naturally present in mice bloodstream. Moreover, by using an orthotopic mouse model, we show that EntF* promotes colorectal cancer metastasis in vivo, with metastatic lesions in liver and lung tissues. In vitro tests suggest that EntF* regulates E-cadherin expression and consequently the epithelial-mesenchymal transition, via the CXCR4 receptor. In addition, alanine-scanning analysis indicates that the first, second, sixth, and tenth amino acid of EntF* are critical for epithelial-mesenchymal transition and tumor metastasis.</jats:p> </jats:sec><jats:sec> <jats:title>Conclusion</jats:title> <jats:p>Our work identifies a new class of molecules, quorum sensing peptides, as potential regulators of host-microbe interactions. We prove, for the first time, the presence of a selected quorum sensing peptide metabolite in a mouse model, and we demonstrate its effects on colorectal cancer metastasis. We believe that our work represents a starting point for future investigations on the role of microbiome in colorectal cancer metastasis and for the development of novel bio-therapeutics in other disease areas.</jats:p> </jats:sec>
Keywords
Cell Biology, Developmental Biology, Plant Science, General Agricultural and Biological Sciences, General Biochemistry, Genetics and Molecular Biology, Physiology, Ecology, Evolution, Behavior and Systematics, Structural Biology, Biotechnology

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MLA
Wynendaele, Evelien, et al. “The Quorum Sensing Peptide EntF* Promotes Colorectal Cancer Metastasis in Mice: A New Factor in the Host-Microbiome Interaction.” BMC Biology, 2022, doi:10.1186/s12915-022-01317-z.
APA
Wynendaele, E., Debunne, N., Janssens, Y., De Spiegeleer, A., Verbeke, F., Tack, L., … De Spiegeleer, B. (2022). The quorum sensing peptide EntF* promotes colorectal cancer metastasis in mice: a new factor in the host-microbiome interaction. BMC Biology. https://doi.org/10.1186/s12915-022-01317-z
Chicago author-date
Wynendaele, Evelien, Nathan Debunne, Yorick Janssens, Anton De Spiegeleer, Frederick Verbeke, Liesa Tack, Sophie Van Welden, et al. 2022. “The Quorum Sensing Peptide EntF* Promotes Colorectal Cancer Metastasis in Mice: A New Factor in the Host-Microbiome Interaction.” BMC Biology. https://doi.org/10.1186/s12915-022-01317-z.
Chicago author-date (all authors)
Wynendaele, Evelien, Nathan Debunne, Yorick Janssens, Anton De Spiegeleer, Frederick Verbeke, Liesa Tack, Sophie Van Welden, Evy Goossens, Daniel Knappe, Ralf Hoffmann, Christophe Van De Wiele, Debby Laukens, Peter Van Eenoo, Lars Vereecke, Filip Van Immerseel, Olivier De Wever, and Bart De Spiegeleer. 2022. “The Quorum Sensing Peptide EntF* Promotes Colorectal Cancer Metastasis in Mice: A New Factor in the Host-Microbiome Interaction.” BMC Biology. doi:10.1186/s12915-022-01317-z.
Vancouver
1.
Wynendaele E, Debunne N, Janssens Y, De Spiegeleer A, Verbeke F, Tack L, et al. The quorum sensing peptide EntF* promotes colorectal cancer metastasis in mice: a new factor in the host-microbiome interaction. BMC Biology. 2022;
IEEE
[1]
E. Wynendaele et al., “The quorum sensing peptide EntF* promotes colorectal cancer metastasis in mice: a new factor in the host-microbiome interaction,” BMC Biology, 2022.
@article{8763504,
  abstract     = {{<jats:title>Abstract</jats:title><jats:sec>
                <jats:title>Background</jats:title>
                <jats:p>Colorectal cancer, one of the most common malignancies worldwide, is associated with a high mortality rate, mainly caused by metastasis. Comparative metagenome-wide association analyses of healthy individuals and cancer patients suggest a role for the human intestinal microbiota in tumor progression. However, the microbial molecules involved in host-microbe communication are largely unknown, with current studies mainly focusing on short-chain fatty acids and amino acid metabolites as potential mediators. Quorum sensing peptides are not yet considered in this context since their presence in vivo and their ability to affect host cells have not been reported so far.</jats:p>
              </jats:sec><jats:sec>
                <jats:title>Results</jats:title>
                <jats:p>Here, we show that EntF*, a metabolite of the quorum sensing peptide EntF produced by <jats:italic>Enterococcus faecium</jats:italic>, is naturally present in mice bloodstream. Moreover, by using an orthotopic mouse model, we show that EntF* promotes colorectal cancer metastasis in vivo, with metastatic lesions in liver and lung tissues. In vitro tests suggest that EntF* regulates E-cadherin expression and consequently the epithelial-mesenchymal transition, via the CXCR4 receptor. In addition, alanine-scanning analysis indicates that the first, second, sixth, and tenth amino acid of EntF* are critical for epithelial-mesenchymal transition and tumor metastasis.</jats:p>
              </jats:sec><jats:sec>
                <jats:title>Conclusion</jats:title>
                <jats:p>Our work identifies a new class of molecules, quorum sensing peptides, as potential regulators of host-microbe interactions. We prove, for the first time, the presence of a selected quorum sensing peptide metabolite in a mouse model, and we demonstrate its effects on colorectal cancer metastasis. We believe that our work represents a starting point for future investigations on the role of microbiome in colorectal cancer metastasis and for the development of novel bio-therapeutics in other disease areas.</jats:p>
              </jats:sec>}},
  author       = {{Wynendaele, Evelien and Debunne, Nathan and Janssens, Yorick and De Spiegeleer, Anton and Verbeke, Frederick and Tack, Liesa and Van Welden, Sophie and Goossens, Evy and Knappe, Daniel and Hoffmann, Ralf and Van De Wiele, Christophe and Laukens, Debby and Van Eenoo, Peter and Vereecke, Lars and Van Immerseel, Filip and De Wever, Olivier and De Spiegeleer, Bart}},
  issn         = {{1741-7007}},
  journal      = {{BMC Biology}},
  keywords     = {{Cell Biology,Developmental Biology,Plant Science,General Agricultural and Biological Sciences,General Biochemistry,Genetics and Molecular Biology,Physiology,Ecology,Evolution,Behavior and Systematics,Structural Biology,Biotechnology}},
  language     = {{eng}},
  title        = {{The quorum sensing peptide EntF* promotes colorectal cancer metastasis in mice: a new factor in the host-microbiome interaction}},
  url          = {{http://dx.doi.org/10.1186/s12915-022-01317-z}},
  year         = {{2022}},
}

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