Academic Bibliography

 Search 200 years of publications by Ghent University researchers.
Advanced search
1 file | 3.24 MB
Add to list
  1. Search results
  2. Conversion of the free Cellvibrio jap...

Conversion of the free Cellvibrio japonicus xyloglucan degradation system to the cellulosomal mode

Julie Vanderstraeten (UGent) , Babette Lamote (UGent) , Maria João de Carvalho Maurício da Fonseca (UGent) , Philippe De Groote (UGent) and Yves Briers (UGent)
(2022) APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 106(17). p.5495-5509
Author
Organization
Project
Abstract
Cellulosomes are multi-enzyme complexes produced by specialised micro-organisms. The spatial proximity of synergistically acting enzymes incorporated in these naturally occurring complexes supports the efficient hydrolysis of lignocellulosic biomass. Several functional designer cellulosomes, incorporating naturally non-cellulosomal cellulases, have been constructed and can be used for cellulose saccharification. However, in lignocellulosic biomass, cellulose is tightly intertwined with several hemicelluloses and lignin. One of the most abundant hemicelluloses interacting with cellulose microfibrils is xyloglucan, and degradation of these polymers is crucial for complete saccharification. Yet, designer cellulosome studies focusing on the incorporation of hemicellulases have been limited. Here, we report the conversion of the free Cellvibrio japonicus xyloglucan degradation system to the cellulosomal mode. Therefore, we constructed multiple docking enzyme variants of C. japonicus endoxyloglucanase, beta-1,2-galactosidase, alpha-1,6 xylosidase and beta-1,4-glucosidase, using the combinatorial VersaTile technique dedicated to the design and optimisation of modular proteins. We individually optimised the docking enzymes to degrade the xyloglucan backbone and side chains. Finally, we show that a purified designer xyloglucanosome comprising these docking enzymes was able to release xyloglucan oligosaccharides, galactose, xylose and glucose from tamarind xyloglucan.
Keywords
Designer cellulosome, Multi-enzyme complex, Hemicellulase, Xyloglucan, VersaTile, DNA assembly, DESIGNER CELLULOSOMES, INTEGRATION, EXPRESSION, PROTEINS, PROMOTES, ENZYMES

Downloads

  • Download
    (...).pdf
    • full text (Published version)
    • |
    • UGent only
    • |
    • PDF
    • |
    • 3.24 MB

Citation

Please use this url to cite or link to this publication:

MLA
Vanderstraeten, Julie, et al. “Conversion of the Free Cellvibrio Japonicus Xyloglucan Degradation System to the Cellulosomal Mode.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 106, no. 17, 2022, pp. 5495–509, doi:10.1007/s00253-022-12072-0.
APA
Vanderstraeten, J., Lamote, B., de Carvalho Maurício da Fonseca, M. J., De Groote, P., & Briers, Y. (2022). Conversion of the free Cellvibrio japonicus xyloglucan degradation system to the cellulosomal mode. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 106(17), 5495–5509. https://doi.org/10.1007/s00253-022-12072-0
Chicago author-date
Vanderstraeten, Julie, Babette Lamote, Maria João de Carvalho Maurício da Fonseca, Philippe De Groote, and Yves Briers. 2022. “Conversion of the Free Cellvibrio Japonicus Xyloglucan Degradation System to the Cellulosomal Mode.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 106 (17): 5495–5509. https://doi.org/10.1007/s00253-022-12072-0.
Chicago author-date (all authors)
Vanderstraeten, Julie, Babette Lamote, Maria João de Carvalho Maurício da Fonseca, Philippe De Groote, and Yves Briers. 2022. “Conversion of the Free Cellvibrio Japonicus Xyloglucan Degradation System to the Cellulosomal Mode.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 106 (17): 5495–5509. doi:10.1007/s00253-022-12072-0.
Vancouver
1.
Vanderstraeten J, Lamote B, de Carvalho Maurício da Fonseca MJ, De Groote P, Briers Y. Conversion of the free Cellvibrio japonicus xyloglucan degradation system to the cellulosomal mode. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2022;106(17):5495–509.
IEEE
[1]
J. Vanderstraeten, B. Lamote, M. J. de Carvalho Maurício da Fonseca, P. De Groote, and Y. Briers, “Conversion of the free Cellvibrio japonicus xyloglucan degradation system to the cellulosomal mode,” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 106, no. 17, pp. 5495–5509, 2022.
@article{8762899,
  abstract     = {{Cellulosomes are multi-enzyme complexes produced by specialised micro-organisms. The spatial proximity of synergistically acting enzymes incorporated in these naturally occurring complexes supports the efficient hydrolysis of lignocellulosic biomass. Several functional designer cellulosomes, incorporating naturally non-cellulosomal cellulases, have been constructed and can be used for cellulose saccharification. However, in lignocellulosic biomass, cellulose is tightly intertwined with several hemicelluloses and lignin. One of the most abundant hemicelluloses interacting with cellulose microfibrils is xyloglucan, and degradation of these polymers is crucial for complete saccharification. Yet, designer cellulosome studies focusing on the incorporation of hemicellulases have been limited. Here, we report the conversion of the free Cellvibrio japonicus xyloglucan degradation system to the cellulosomal mode. Therefore, we constructed multiple docking enzyme variants of C. japonicus endoxyloglucanase, beta-1,2-galactosidase, alpha-1,6 xylosidase and beta-1,4-glucosidase, using the combinatorial VersaTile technique dedicated to the design and optimisation of modular proteins. We individually optimised the docking enzymes to degrade the xyloglucan backbone and side chains. Finally, we show that a purified designer xyloglucanosome comprising these docking enzymes was able to release xyloglucan oligosaccharides, galactose, xylose and glucose from tamarind xyloglucan.}},
  author       = {{Vanderstraeten, Julie and Lamote, Babette and de Carvalho Maurício da Fonseca, Maria João and De Groote, Philippe and Briers, Yves}},
  issn         = {{0175-7598}},
  journal      = {{APPLIED MICROBIOLOGY AND BIOTECHNOLOGY}},
  keywords     = {{Designer cellulosome,Multi-enzyme complex,Hemicellulase,Xyloglucan,VersaTile,DNA assembly,DESIGNER CELLULOSOMES,INTEGRATION,EXPRESSION,PROTEINS,PROMOTES,ENZYMES}},
  language     = {{eng}},
  number       = {{17}},
  pages        = {{5495--5509}},
  title        = {{Conversion of the free Cellvibrio japonicus xyloglucan degradation system to the cellulosomal mode}},
  url          = {{http://doi.org/10.1007/s00253-022-12072-0}},
  volume       = {{106}},
  year         = {{2022}},
}
Altmetric
View in Altmetric
Web of Science
Times cited: