Academic Bibliography

 Search 200 years of publications by Ghent University researchers.
Advanced search
1 file | 2.20 MB
Add to list
  1. Search results
  2. Altering in vivo membrane sterol comp...

Altering in vivo membrane sterol composition affects the activity of the cyclic lipopeptides tolaasin and sessilin against Pythium

Enrico Ferrarini (UGent) , Vic De Roo, Niels Geudens (UGent) , José Martins (UGent) and Monica Höfte (UGent)
(2022) BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES. 1864(10).
Author
Organization
Project
Abstract
Cyclic lipopeptides (CLiPs) are secondary metabolites produced by a variety of bacteria. These compounds show a broad range of antimicrobial activities; therefore, they are studied for their potential applications in agriculture and medicine. It is generally assumed that the primary target of the CLiPs is the cellular membrane, where they can permeabilize the lipid bilayer. Model membrane systems are commonly used to investigate the effect of lipid composition on the permeabilizing activity of CLiPs, but these systems do not represent the full complexity of true biological membranes. Here, we introduce a novel method that uses sterol-auxotrophic oomycetes to investigate how the activity of membrane-active compounds is influenced by alterations in membrane sterol composition. More specifically, we investigated how ergosterol, cholesterol, beta-sitosterol and stigmasterol affect the activity of the structurally related Pseudomonas-derived CLiPs tolaasin and sessilin against the oomycete Pythium myriotylum. Both compounds were effective against oomycetes, although tolaasin was considerably more active. Interestingly, tolaasin and sessilin effects were similarly reduced by the presence of sterols, with cholesterol showing the highest reduction of activity.
Keywords
Cell Biology, Biochemistry, Biophysics, Pythium, Pseudomonas, Oomycetes, Membranes, Sterols, Sessilin, Tolaasin, lipopeptides, Cyclic

Downloads

  • Download
    (...).pdf
    • full text (Published version)
    • |
    • UGent only
    • |
    • PDF
    • |
    • 2.20 MB

Citation

Please use this url to cite or link to this publication:

MLA
Ferrarini, Enrico, et al. “Altering in Vivo Membrane Sterol Composition Affects the Activity of the Cyclic Lipopeptides Tolaasin and Sessilin against Pythium.” BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, vol. 1864, no. 10, 2022, doi:10.1016/j.bbamem.2022.184008.
APA
Ferrarini, E., De Roo, V., Geudens, N., Martins, J., & Höfte, M. (2022). Altering in vivo membrane sterol composition affects the activity of the cyclic lipopeptides tolaasin and sessilin against Pythium. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1864(10). https://doi.org/10.1016/j.bbamem.2022.184008
Chicago author-date
Ferrarini, Enrico, Vic De Roo, Niels Geudens, José Martins, and Monica Höfte. 2022. “Altering in Vivo Membrane Sterol Composition Affects the Activity of the Cyclic Lipopeptides Tolaasin and Sessilin against Pythium.” BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1864 (10). https://doi.org/10.1016/j.bbamem.2022.184008.
Chicago author-date (all authors)
Ferrarini, Enrico, Vic De Roo, Niels Geudens, José Martins, and Monica Höfte. 2022. “Altering in Vivo Membrane Sterol Composition Affects the Activity of the Cyclic Lipopeptides Tolaasin and Sessilin against Pythium.” BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1864 (10). doi:10.1016/j.bbamem.2022.184008.
Vancouver
1.
Ferrarini E, De Roo V, Geudens N, Martins J, Höfte M. Altering in vivo membrane sterol composition affects the activity of the cyclic lipopeptides tolaasin and sessilin against Pythium. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES. 2022;1864(10).
IEEE
[1]
E. Ferrarini, V. De Roo, N. Geudens, J. Martins, and M. Höfte, “Altering in vivo membrane sterol composition affects the activity of the cyclic lipopeptides tolaasin and sessilin against Pythium,” BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, vol. 1864, no. 10, 2022.
@article{8762148,
  abstract     = {{Cyclic lipopeptides (CLiPs) are secondary metabolites produced by a variety of bacteria. These compounds show a broad range of antimicrobial activities; therefore, they are studied for their potential applications in agriculture and medicine. It is generally assumed that the primary target of the CLiPs is the cellular membrane, where they can permeabilize the lipid bilayer. Model membrane systems are commonly used to investigate the effect of lipid composition on the permeabilizing activity of CLiPs, but these systems do not represent the full complexity of true biological membranes. Here, we introduce a novel method that uses sterol-auxotrophic oomycetes to investigate how the activity of membrane-active compounds is influenced by alterations in membrane sterol composition. More specifically, we investigated how ergosterol, cholesterol, beta-sitosterol and stigmasterol affect the activity of the structurally related Pseudomonas-derived CLiPs tolaasin and sessilin against the oomycete Pythium myriotylum. Both compounds were effective against oomycetes, although tolaasin was considerably more active. Interestingly, tolaasin and sessilin effects were similarly reduced by the presence of sterols, with cholesterol showing the highest reduction of activity.}},
  articleno    = {{184008}},
  author       = {{Ferrarini, Enrico and De Roo, Vic and Geudens, Niels and Martins, José and Höfte, Monica}},
  issn         = {{0005-2736}},
  journal      = {{BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES}},
  keywords     = {{Cell Biology,Biochemistry,Biophysics,Pythium,Pseudomonas,Oomycetes,Membranes,Sterols,Sessilin,Tolaasin,lipopeptides,Cyclic}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{10}},
  title        = {{Altering in vivo membrane sterol composition affects the activity of the cyclic lipopeptides tolaasin and sessilin against Pythium}},
  url          = {{http://doi.org/10.1016/j.bbamem.2022.184008}},
  volume       = {{1864}},
  year         = {{2022}},
}
Altmetric
View in Altmetric
Web of Science
Times cited: