Overexpression of the scopoletin biosynthetic pathway enhances lignocellulosic biomass processing
- Author
- Lennart Hoengenaert (UGent) , Marlies Wouters, Hoon Kim, Barbara De Meester (UGent) , Kris Morreel (UGent) , Steven Vandersyppe (UGent) , Jacob Pollier (UGent) , Sandrien Desmet (UGent) , Geert Goeminne (UGent) , John Ralph, Wout Boerjan (UGent) and Ruben Vanholme (UGent)
- Organization
- Project
-
- Optimisation of the lignin composition of poplar to improve biomass processing in favor of a bio-based economy.
- Next generation lignocellulosics biorefinery concepts and implementation (Next-BIOREF)
- Unraveling the function of new O-methyltransferases in phenylpropanoid metabolism
- Optimalisatie van populier voor een bio-gebaseerde economie door middel van weefselspecifieke lignificatie en de incorporatie van alternatieve ligninemonomeren
- Pathway discovery in ferulic acid metabolism with relevance to lignin biosynthesis
- Developing transgene-free genome-engineered poplars to enabling field applications for the biobased economy.
- Biorefinery of entire plant biomass to aromatics
- Exploring and broadening the valorization potential of ugt72e-mediated growth restoration of low-lignin plants
- Abstract
- Lignin is the main factor limiting the enzymatic conversion of lignocellulosic biomass into fermentable sugars. To reduce the recalcitrance engendered by the lignin polymer, the coumarin scopoletin was incorporated into the lignin polymer through the simultaneous expression of FERULOYL-CoA 6'-HYDROXYLASE 1 (F6'H1) and COUMARIN SYNTHASE (COSY) in lignifying cells in Arabidopsis. The transgenic lines overproduced scopoletin and incorporated it into the lignin polymer, without adversely affecting plant growth. About 3.3% of the lignin units in the transgenic lines were derived from scopoletin, thereby exceeding the levels of the traditional p-hydroxyphenyl units. Saccharification efficiency of alkali-pretreated scopoletin-overproducing lines was 40% higher than for wild type.
- Keywords
- Multidisciplinary, LIGNIN MONOMER COMPOSITION, ARABIDOPSIS, POPLAR, EXPRESSION, CLEAVAGE, ETHER, COA, PERTURBATIONS, DEFICIENT, LINKAGES
Downloads
-
Hoengenaert et al. 2022 Science Advances 8 eabo5738.pdf
- full text (Published version)
- |
- open access
- |
- |
- 2.57 MB
-
Data S2 v4.pdf
- supplementary material
- |
- open access
- |
- |
- 962.87 KB
-
Data S3.pdf
- supplementary material
- |
- open access
- |
- |
- 253.81 KB
-
abo5738 SupplementalMaterial No fluo.docx
- supplementary material
- |
- open access
- |
- Word
- |
- 8.99 MB
-
Data S1.pdf
- supplementary material
- |
- open access
- |
- |
- 662.36 KB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8761962
- MLA
- Hoengenaert, Lennart, et al. “Overexpression of the Scopoletin Biosynthetic Pathway Enhances Lignocellulosic Biomass Processing.” SCIENCE ADVANCES, vol. 8, no. 28, 2022, doi:10.1126/sciadv.abo5738.
- APA
- Hoengenaert, L., Wouters, M., Kim, H., De Meester, B., Morreel, K., Vandersyppe, S., … Vanholme, R. (2022). Overexpression of the scopoletin biosynthetic pathway enhances lignocellulosic biomass processing. SCIENCE ADVANCES, 8(28). https://doi.org/10.1126/sciadv.abo5738
- Chicago author-date
- Hoengenaert, Lennart, Marlies Wouters, Hoon Kim, Barbara De Meester, Kris Morreel, Steven Vandersyppe, Jacob Pollier, et al. 2022. “Overexpression of the Scopoletin Biosynthetic Pathway Enhances Lignocellulosic Biomass Processing.” SCIENCE ADVANCES 8 (28). https://doi.org/10.1126/sciadv.abo5738.
- Chicago author-date (all authors)
- Hoengenaert, Lennart, Marlies Wouters, Hoon Kim, Barbara De Meester, Kris Morreel, Steven Vandersyppe, Jacob Pollier, Sandrien Desmet, Geert Goeminne, John Ralph, Wout Boerjan, and Ruben Vanholme. 2022. “Overexpression of the Scopoletin Biosynthetic Pathway Enhances Lignocellulosic Biomass Processing.” SCIENCE ADVANCES 8 (28). doi:10.1126/sciadv.abo5738.
- Vancouver
- 1.Hoengenaert L, Wouters M, Kim H, De Meester B, Morreel K, Vandersyppe S, et al. Overexpression of the scopoletin biosynthetic pathway enhances lignocellulosic biomass processing. SCIENCE ADVANCES. 2022;8(28).
- IEEE
- [1]L. Hoengenaert et al., “Overexpression of the scopoletin biosynthetic pathway enhances lignocellulosic biomass processing,” SCIENCE ADVANCES, vol. 8, no. 28, 2022.
@article{8761962,
abstract = {{Lignin is the main factor limiting the enzymatic conversion of lignocellulosic biomass into fermentable sugars. To reduce the recalcitrance engendered by the lignin polymer, the coumarin scopoletin was incorporated into the lignin polymer through the simultaneous expression of FERULOYL-CoA 6'-HYDROXYLASE 1 (F6'H1) and COUMARIN SYNTHASE (COSY) in lignifying cells in Arabidopsis. The transgenic lines overproduced scopoletin and incorporated it into the lignin polymer, without adversely affecting plant growth. About 3.3% of the lignin units in the transgenic lines were derived from scopoletin, thereby exceeding the levels of the traditional p-hydroxyphenyl units. Saccharification efficiency of alkali-pretreated scopoletin-overproducing lines was 40% higher than for wild type.}},
articleno = {{eabo5738}},
author = {{Hoengenaert, Lennart and Wouters, Marlies and Kim, Hoon and De Meester, Barbara and Morreel, Kris and Vandersyppe, Steven and Pollier, Jacob and Desmet, Sandrien and Goeminne, Geert and Ralph, John and Boerjan, Wout and Vanholme, Ruben}},
issn = {{2375-2548}},
journal = {{SCIENCE ADVANCES}},
keywords = {{Multidisciplinary,LIGNIN MONOMER COMPOSITION,ARABIDOPSIS,POPLAR,EXPRESSION,CLEAVAGE,ETHER,COA,PERTURBATIONS,DEFICIENT,LINKAGES}},
language = {{eng}},
number = {{28}},
pages = {{12}},
title = {{Overexpression of the scopoletin biosynthetic pathway enhances lignocellulosic biomass processing}},
url = {{http://doi.org/10.1126/sciadv.abo5738}},
volume = {{8}},
year = {{2022}},
}
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: