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Overexpression of the scopoletin biosynthetic pathway enhances lignocellulosic biomass processing

Lennart Hoengenaert (UGent) , Marlies Wouters (UGent) , Hoon Kim, Barbara De Meester (UGent) , Kris Morreel (UGent) , Steven Vandersyppe (UGent) , Jacob Pollier (UGent) , Sandrien Desmet (UGent) , Geert Goeminne (UGent) , John Ralph, et al.
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Abstract
Lignin is the main factor limiting the enzymatic conversion of lignocellulosic biomass into fermentable sugars. To reduce the recalcitrance engendered by the lignin polymer, the coumarin scopoletin was incorporated into the lignin polymer through the simultaneous expression of FERULOYL-CoA 6'-HYDROXYLASE 1 (F6'H1) and COUMARIN SYNTHASE (COSY) in lignifying cells in Arabidopsis. The transgenic lines overproduced scopoletin and incorporated it into the lignin polymer, without adversely affecting plant growth. About 3.3% of the lignin units in the transgenic lines were derived from scopoletin, thereby exceeding the levels of the traditional p-hydroxyphenyl units. Saccharification efficiency of alkali-pretreated scopoletin-overproducing lines was 40% higher than for wild type.
Keywords
Multidisciplinary, LIGNIN MONOMER COMPOSITION, ARABIDOPSIS, POPLAR, EXPRESSION, CLEAVAGE, ETHER, COA, PERTURBATIONS, DEFICIENT, LINKAGES

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Citation

Please use this url to cite or link to this publication:

MLA
Hoengenaert, Lennart, et al. “Overexpression of the Scopoletin Biosynthetic Pathway Enhances Lignocellulosic Biomass Processing.” SCIENCE ADVANCES, vol. 8, no. 28, 2022, doi:10.1126/sciadv.abo5738.
APA
Hoengenaert, L., Wouters, M., Kim, H., De Meester, B., Morreel, K., Vandersyppe, S., … Vanholme, R. (2022). Overexpression of the scopoletin biosynthetic pathway enhances lignocellulosic biomass processing. SCIENCE ADVANCES, 8(28). https://doi.org/10.1126/sciadv.abo5738
Chicago author-date
Hoengenaert, Lennart, Marlies Wouters, Hoon Kim, Barbara De Meester, Kris Morreel, Steven Vandersyppe, Jacob Pollier, et al. 2022. “Overexpression of the Scopoletin Biosynthetic Pathway Enhances Lignocellulosic Biomass Processing.” SCIENCE ADVANCES 8 (28). https://doi.org/10.1126/sciadv.abo5738.
Chicago author-date (all authors)
Hoengenaert, Lennart, Marlies Wouters, Hoon Kim, Barbara De Meester, Kris Morreel, Steven Vandersyppe, Jacob Pollier, Sandrien Desmet, Geert Goeminne, John Ralph, Wout Boerjan, and Ruben Vanholme. 2022. “Overexpression of the Scopoletin Biosynthetic Pathway Enhances Lignocellulosic Biomass Processing.” SCIENCE ADVANCES 8 (28). doi:10.1126/sciadv.abo5738.
Vancouver
1.
Hoengenaert L, Wouters M, Kim H, De Meester B, Morreel K, Vandersyppe S, et al. Overexpression of the scopoletin biosynthetic pathway enhances lignocellulosic biomass processing. SCIENCE ADVANCES. 2022;8(28).
IEEE
[1]
L. Hoengenaert et al., “Overexpression of the scopoletin biosynthetic pathway enhances lignocellulosic biomass processing,” SCIENCE ADVANCES, vol. 8, no. 28, 2022.
@article{8761962,
  abstract     = {{Lignin is the main factor limiting the enzymatic conversion of lignocellulosic biomass into fermentable sugars. To reduce the recalcitrance engendered by the lignin polymer, the coumarin scopoletin was incorporated into the lignin polymer through the simultaneous expression of FERULOYL-CoA 6'-HYDROXYLASE 1 (F6'H1) and COUMARIN SYNTHASE (COSY) in lignifying cells in Arabidopsis. The transgenic lines overproduced scopoletin and incorporated it into the lignin polymer, without adversely affecting plant growth. About 3.3% of the lignin units in the transgenic lines were derived from scopoletin, thereby exceeding the levels of the traditional p-hydroxyphenyl units. Saccharification efficiency of alkali-pretreated scopoletin-overproducing lines was 40% higher than for wild type.}},
  articleno    = {{eabo5738}},
  author       = {{Hoengenaert, Lennart and Wouters, Marlies and Kim, Hoon and De Meester, Barbara and Morreel, Kris and Vandersyppe, Steven and Pollier, Jacob and Desmet, Sandrien and Goeminne, Geert and Ralph, John and Boerjan, Wout and Vanholme, Ruben}},
  issn         = {{2375-2548}},
  journal      = {{SCIENCE ADVANCES}},
  keywords     = {{Multidisciplinary,LIGNIN MONOMER COMPOSITION,ARABIDOPSIS,POPLAR,EXPRESSION,CLEAVAGE,ETHER,COA,PERTURBATIONS,DEFICIENT,LINKAGES}},
  language     = {{eng}},
  number       = {{28}},
  pages        = {{12}},
  title        = {{Overexpression of the scopoletin biosynthetic pathway enhances lignocellulosic biomass processing}},
  url          = {{http://dx.doi.org/10.1126/sciadv.abo5738}},
  volume       = {{8}},
  year         = {{2022}},
}

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