@inproceedings{8761764,
  abstract     = {{Temperature-responsive liquid chromatography (TRLC) allows for extensive retention and selectivity tuning through
temperature in HPLC. This is achieved through the use of stationary phases comprising a temperature-responsive
polymer which is able to undergo a reversible change of its surface from hydrophilic to hydrophobic upon an increase
of the temperature. This interesting behaviour allows for retention behaviour to be controlled purely by a change in
temperature,1,2 allowing for conventionally used mobile phase gradients to be replaced by temperature gradients.
As a result, this technique allows for analyses to be performed under purely aqueous conditions.3,4 However, despite
the promising nature of such form of retention control under isocratic mobile phase conditions, TRLC can suffer from
excessive retention upon analysis of highly apolar solutes even at lower column temperatures where the surface of
the polymer behaves hydrophilic. This is related both to a residual apolarity of the polymer chain, as well as due
to the high inherent logP values and reduced water solubility of the more highly apolar compounds. Despite this
apparent drawback, analyses in TRLC have already been performed under non-purely aqueous conditions and the use
of co-solvents to the water have been proven to be possible.5–7 However, the exact impact for introduction of organic
solvents was not yet investigated in a systematic way. Therefore, in this study the advantages and drawbacks for the
use of organic co-solvents (methanol and acetonitrile) in TRLC was assessed on two types of temperature-responsive
phases: poly-N-N-isopropylacrylamide (PNIPAAm) and poly-N-isopropylacrylamide (PNNPAAm). For this, the
influence of organic co-solvents on the efficiency of the stationary phase as well as the cloud point temperature of
the polymers is investigated with two representative test mixtures comprising of parabens and apolar steroids}},
  author       = {{Ampe, Adriaan}},
  booktitle    = {{17th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-17), Abstracts}},
  keywords     = {{temperature-responsive chromatography,mixed mobile phase,PNIPAAm}},
  language     = {{eng}},
  location     = {{Ghent, Belgium}},
  title        = {{Investigation of the potential of mixed solvent mobile phases in temperature-responsive liquid chromatography (TRLC)}},
  year         = {{2022}},
}