Field and saccharification performances of poplars severely downregulated in CAD1
- Author
- Barbara De Meester (UGent) , Rebecca Van Acker (UGent) , Marlies Wouters, Silvia Traversari, Marijke Steenackers (UGent) , Jenny Neukermans (UGent) , Frank Van Breusegem (UGent) , Annabelle Déjardin, Gilles Pilate and Wout Boerjan (UGent)
- Organization
- Project
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- Unraveling the function of new O-methyltransferases in phenylpropanoid metabolism
- ADV_BIO: Technological innovation in the production of advanced biofuels applicable to the Belgian territory for road and air transport and techno-economic and environmental analyses
- Advanced Liquid Biofuels for advaced engine concepts enabled by advanced wood breeding and catalysis
- BIOLEUM: Brandstoffen en chemicaliën door snelle pyrolyse van biomassa
- Exploring and broadening the valorization potential of ugt72e-mediated growth restoration of low-lignin plants
- Abstract
- •Lignin is one of the main factors causing lignocellulosic biomass recalcitrance to enzymatic hydrolysis. Greenhouse-grown poplars severely downregulated for CINNAMYL ALCOHOL DEHYDROGENASE 1 (CAD1), the enzyme catalyzing the last step in the monolignol-specific branch of lignin biosynthesis, have increased saccharification yields and normal growth. •Here, we assess the performance of these hpCAD poplars in the field under short rotation coppice culture for two consecutive rotations of one and three years. •While one-year-old hpCAD wood had 10% less lignin, three-year-old hpCAD wood had wild-type lignin levels. Because of their altered cell wall composition, including elevated levels of cinnamaldehydes, both one- and three-year-old hpCAD wood showed enhanced saccharification yields upon harsh alkaline pretreatments (up to +85% and +77%, respectively). In contrast to previous field trials with poplars less severely downregulated for CAD, the hpCAD poplars displayed leaning phenotypes, early bud set, early flowering and yield penalties. Moreover, hpCAD wood had enlarged vessels, decreased wood density, and reduced relative and free water contents. •Our data show that the phenotypes of CAD-deficient poplars are strongly dependent on the environment and underpin the importance of field trials in translating basic research towards applications.
- Keywords
- CAD, field trial, lignin engineering, poplar, saccharification, water content, wood, CINNAMYL-ALCOHOL-DEHYDROGENASE, LIGNIN BIOSYNTHESIS, TRANSGENIC POPLARS, MECHANICAL-PROPERTIES, POPULUS-TRICHOCARPA, HYBRID POPLAR, TRADE-OFF, XYLEM, GROWTH, PLANTS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8761567
- MLA
- De Meester, Barbara, et al. “Field and Saccharification Performances of Poplars Severely Downregulated in CAD1.” NEW PHYTOLOGIST, vol. 236, no. 6, 2022, pp. 2075–90, doi:10.1111/nph.18366.
- APA
- De Meester, B., Van Acker, R., Wouters, M., Traversari, S., Steenackers, M., Neukermans, J., … Boerjan, W. (2022). Field and saccharification performances of poplars severely downregulated in CAD1. NEW PHYTOLOGIST, 236(6), 2075–2090. https://doi.org/10.1111/nph.18366
- Chicago author-date
- De Meester, Barbara, Rebecca Van Acker, Marlies Wouters, Silvia Traversari, Marijke Steenackers, Jenny Neukermans, Frank Van Breusegem, Annabelle Déjardin, Gilles Pilate, and Wout Boerjan. 2022. “Field and Saccharification Performances of Poplars Severely Downregulated in CAD1.” NEW PHYTOLOGIST 236 (6): 2075–90. https://doi.org/10.1111/nph.18366.
- Chicago author-date (all authors)
- De Meester, Barbara, Rebecca Van Acker, Marlies Wouters, Silvia Traversari, Marijke Steenackers, Jenny Neukermans, Frank Van Breusegem, Annabelle Déjardin, Gilles Pilate, and Wout Boerjan. 2022. “Field and Saccharification Performances of Poplars Severely Downregulated in CAD1.” NEW PHYTOLOGIST 236 (6): 2075–2090. doi:10.1111/nph.18366.
- Vancouver
- 1.De Meester B, Van Acker R, Wouters M, Traversari S, Steenackers M, Neukermans J, et al. Field and saccharification performances of poplars severely downregulated in CAD1. NEW PHYTOLOGIST. 2022;236(6):2075–90.
- IEEE
- [1]B. De Meester et al., “Field and saccharification performances of poplars severely downregulated in CAD1,” NEW PHYTOLOGIST, vol. 236, no. 6, pp. 2075–2090, 2022.
@article{8761567,
abstract = {{•Lignin is one of the main factors causing lignocellulosic biomass recalcitrance to enzymatic hydrolysis. Greenhouse-grown poplars severely downregulated for CINNAMYL ALCOHOL DEHYDROGENASE 1 (CAD1), the enzyme catalyzing the last step in the monolignol-specific branch of lignin biosynthesis, have increased saccharification yields and normal growth.
•Here, we assess the performance of these hpCAD poplars in the field under short rotation coppice culture for two consecutive rotations of one and three years.
•While one-year-old hpCAD wood had 10% less lignin, three-year-old hpCAD wood had wild-type lignin levels. Because of their altered cell wall composition, including elevated levels of cinnamaldehydes, both one- and three-year-old hpCAD wood showed enhanced saccharification yields upon harsh alkaline pretreatments (up to +85% and +77%, respectively). In contrast to previous field trials with poplars less severely downregulated for CAD, the hpCAD poplars displayed leaning phenotypes, early bud set, early flowering and yield penalties. Moreover, hpCAD wood had enlarged vessels, decreased wood density, and reduced relative and free water contents.
•Our data show that the phenotypes of CAD-deficient poplars are strongly dependent on the environment and underpin the importance of field trials in translating basic research towards applications.}},
author = {{De Meester, Barbara and Van Acker, Rebecca and Wouters, Marlies and Traversari, Silvia and Steenackers, Marijke and Neukermans, Jenny and Van Breusegem, Frank and Déjardin, Annabelle and Pilate, Gilles and Boerjan, Wout}},
issn = {{0028-646X}},
journal = {{NEW PHYTOLOGIST}},
keywords = {{CAD,field trial,lignin engineering,poplar,saccharification,water content,wood,CINNAMYL-ALCOHOL-DEHYDROGENASE,LIGNIN BIOSYNTHESIS,TRANSGENIC POPLARS,MECHANICAL-PROPERTIES,POPULUS-TRICHOCARPA,HYBRID POPLAR,TRADE-OFF,XYLEM,GROWTH,PLANTS}},
language = {{eng}},
number = {{6}},
pages = {{2075--2090}},
title = {{Field and saccharification performances of poplars severely downregulated in CAD1}},
url = {{http://doi.org/10.1111/nph.18366}},
volume = {{236}},
year = {{2022}},
}
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