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Lentils and yeast fibers : a new strategy to mitigate enterotoxigenic Escherichia coli (ETEC) strain H10407 virulence?

Thomas Sauvaitre, Florence Van Herreweghen (UGent) , Karen Delbaere (UGent) , Claude Durif, Josefien Van Landuyt (UGent) , Khaled Fadhlaoui, Segolene Huille, Frederique Chaucheyras-Durand, Lucie Etienne-Mesmin, Stephanie Blanquet-Diot, et al.
(2022) NUTRIENTS. 14(10).
Author
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Abstract
Dietary fibers exhibit well-known beneficial effects on human health, but their anti-infectious properties against enteric pathogens have been poorly investigated. Enterotoxigenic Escherichia coli (ETEC) is a major food-borne pathogen that causes acute traveler's diarrhea. Its virulence traits mainly rely on adhesion to an epithelial surface, mucus degradation, and the secretion of two enterotoxins associated with intestinal inflammation. With the increasing burden of antibiotic resistance worldwide, there is an imperious need to develop novel alternative strategies to control ETEC infections. This study aimed to investigate, using complementary in vitro approaches, the inhibitory potential of two dietary-fiber-containing products (a lentil extract and yeast cell walls) against the human ETEC reference strain H10407. We showed that the lentil extract decreased toxin production in a dose-dependent manner, reduced pro-inflammatory interleukin-8 production, and modulated mucus-related gene induction in ETEC-infected mucus-secreting intestinal cells. We also report that the yeast product reduced ETEC adhesion to mucin and Caco-2/HT29-MTX cells. Both fiber-containing products strengthened intestinal barrier function and modulated toxin-related gene expression. In a complex human gut microbial background, both products did not elicit a significant effect on ETEC colonization. These pioneering data demonstrate the promising role of dietary fibers in controlling different stages of the ETEC infection process.
Keywords
HEAT-STABLE ENTEROTOXIN, IN-SITU HYBRIDIZATION, DIETARY FIBER, BARRIER, DYSFUNCTION, SECRETED METALLOPROTEASE, COLONIZATION FACTORS, GROWTH-PERFORMANCE, LABILE ENTEROTOXIN, INTESTINAL-TRACT, GUT MICROBIOTA, dietary fiber, enterotoxigenic Escherichia coli, virulence, mucus, fecal, microbiota, innate immune response

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MLA
Sauvaitre, Thomas, et al. “Lentils and Yeast Fibers : A New Strategy to Mitigate Enterotoxigenic Escherichia Coli (ETEC) Strain H10407 Virulence?” NUTRIENTS, vol. 14, no. 10, 2022, doi:10.3390/nu14102146.
APA
Sauvaitre, T., Van Herreweghen, F., Delbaere, K., Durif, C., Van Landuyt, J., Fadhlaoui, K., … Van de Wiele, T. (2022). Lentils and yeast fibers : a new strategy to mitigate enterotoxigenic Escherichia coli (ETEC) strain H10407 virulence? NUTRIENTS, 14(10). https://doi.org/10.3390/nu14102146
Chicago author-date
Sauvaitre, Thomas, Florence Van Herreweghen, Karen Delbaere, Claude Durif, Josefien Van Landuyt, Khaled Fadhlaoui, Segolene Huille, et al. 2022. “Lentils and Yeast Fibers : A New Strategy to Mitigate Enterotoxigenic Escherichia Coli (ETEC) Strain H10407 Virulence?” NUTRIENTS 14 (10). https://doi.org/10.3390/nu14102146.
Chicago author-date (all authors)
Sauvaitre, Thomas, Florence Van Herreweghen, Karen Delbaere, Claude Durif, Josefien Van Landuyt, Khaled Fadhlaoui, Segolene Huille, Frederique Chaucheyras-Durand, Lucie Etienne-Mesmin, Stephanie Blanquet-Diot, and Tom Van de Wiele. 2022. “Lentils and Yeast Fibers : A New Strategy to Mitigate Enterotoxigenic Escherichia Coli (ETEC) Strain H10407 Virulence?” NUTRIENTS 14 (10). doi:10.3390/nu14102146.
Vancouver
1.
Sauvaitre T, Van Herreweghen F, Delbaere K, Durif C, Van Landuyt J, Fadhlaoui K, et al. Lentils and yeast fibers : a new strategy to mitigate enterotoxigenic Escherichia coli (ETEC) strain H10407 virulence? NUTRIENTS. 2022;14(10).
IEEE
[1]
T. Sauvaitre et al., “Lentils and yeast fibers : a new strategy to mitigate enterotoxigenic Escherichia coli (ETEC) strain H10407 virulence?,” NUTRIENTS, vol. 14, no. 10, 2022.
@article{8759981,
  abstract     = {{Dietary fibers exhibit well-known beneficial effects on human health, but their anti-infectious properties against enteric pathogens have been poorly investigated. Enterotoxigenic Escherichia coli (ETEC) is a major food-borne pathogen that causes acute traveler's diarrhea. Its virulence traits mainly rely on adhesion to an epithelial surface, mucus degradation, and the secretion of two enterotoxins associated with intestinal inflammation. With the increasing burden of antibiotic resistance worldwide, there is an imperious need to develop novel alternative strategies to control ETEC infections. This study aimed to investigate, using complementary in vitro approaches, the inhibitory potential of two dietary-fiber-containing products (a lentil extract and yeast cell walls) against the human ETEC reference strain H10407. We showed that the lentil extract decreased toxin production in a dose-dependent manner, reduced pro-inflammatory interleukin-8 production, and modulated mucus-related gene induction in ETEC-infected mucus-secreting intestinal cells. We also report that the yeast product reduced ETEC adhesion to mucin and Caco-2/HT29-MTX cells. Both fiber-containing products strengthened intestinal barrier function and modulated toxin-related gene expression. In a complex human gut microbial background, both products did not elicit a significant effect on ETEC colonization. These pioneering data demonstrate the promising role of dietary fibers in controlling different stages of the ETEC infection process.}},
  articleno    = {{2146}},
  author       = {{Sauvaitre, Thomas and Van Herreweghen, Florence and Delbaere, Karen and Durif, Claude and Van Landuyt, Josefien and Fadhlaoui, Khaled and Huille, Segolene and Chaucheyras-Durand, Frederique and Etienne-Mesmin, Lucie and Blanquet-Diot, Stephanie and Van de Wiele, Tom}},
  issn         = {{2072-6643}},
  journal      = {{NUTRIENTS}},
  keywords     = {{HEAT-STABLE ENTEROTOXIN,IN-SITU HYBRIDIZATION,DIETARY FIBER,BARRIER,DYSFUNCTION,SECRETED METALLOPROTEASE,COLONIZATION FACTORS,GROWTH-PERFORMANCE,LABILE ENTEROTOXIN,INTESTINAL-TRACT,GUT MICROBIOTA,dietary fiber,enterotoxigenic Escherichia coli,virulence,mucus,fecal,microbiota,innate immune response}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{34}},
  title        = {{Lentils and yeast fibers : a new strategy to mitigate enterotoxigenic Escherichia coli (ETEC) strain H10407 virulence?}},
  url          = {{http://doi.org/10.3390/nu14102146}},
  volume       = {{14}},
  year         = {{2022}},
}
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