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lnc94638 is a testis-specific long non-coding RNA involved in spermatozoa formation in Zeugodacus cucurbitae (Coquillett)

W-J Li, D. Wei, H-L Han, Y-J Song, Y. Wang, H-Q Xu, Guy Smagghe (UGent) and J-J Wang
(2021) INSECT MOLECULAR BIOLOGY. 30(6). p.605-614
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Abstract
Long non-coding RNAs (lncRNAs) generally display tissue-specific distributions, and testis-specific lncRNAs form the highest proportion of lncRNAs in many species. Here, we presented a detailed analysis of testis-specific lncRNAs in the melon fly, Zeugodacus cucurbitae, a highly destructive insect pest of cucurbitaceous and other related crops. Most testis-specific lncRNAs were found to be long intergenic non-coding RNAs (lincRNA). The size distribution of these lncRNAs ranged between 600 and 1000 nucleotides. Testis-specific lncRNAs that harboured one isoform number and two exons were the most abundant. Compared to other male tissues, the testis had more highly expressed lncRNAs. The quantitative real-time polymerase chain reaction results of 10 randomly selected testis-specific lncRNAs showed expression patterns consistent with RNA-seq data. Further analysis of the most highly expressed testis-specific lncRNA, lnc94638, was undertaken. Fluorescent in situ hybridization assays localized lnc94638 to the apical region of the testis that contains mature spermatozoa. RNA interference-mediated knockdown of lnc94638 expression reduced spermatozoa numbers and impaired the fertility of Z. cucurbitae male. This study provides a catalogue of testis-specific lncRNAs, shows that the testis-specific lnc94638 is involved in spermatogenesis and has the potential to be used for treating male sterility.
Keywords
IDENTIFICATION, EXPRESSION, ROLES, melon fly, RNA-seq, long non-coding RNA, testis-specific, spermatogenesis, fertility

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MLA
Li, W. J., et al. “Lnc94638 Is a Testis-Specific Long Non-Coding RNA Involved in Spermatozoa Formation in Zeugodacus Cucurbitae (Coquillett).” INSECT MOLECULAR BIOLOGY, vol. 30, no. 6, 2021, pp. 605–14, doi:10.1111/imb.12729.
APA
Li, W.-J., Wei, D., Han, H.-L., Song, Y.-J., Wang, Y., Xu, H.-Q., … Wang, J.-J. (2021). lnc94638 is a testis-specific long non-coding RNA involved in spermatozoa formation in Zeugodacus cucurbitae (Coquillett). INSECT MOLECULAR BIOLOGY, 30(6), 605–614. https://doi.org/10.1111/imb.12729
Chicago author-date
Li, W-J, D. Wei, H-L Han, Y-J Song, Y. Wang, H-Q Xu, Guy Smagghe, and J-J Wang. 2021. “Lnc94638 Is a Testis-Specific Long Non-Coding RNA Involved in Spermatozoa Formation in Zeugodacus Cucurbitae (Coquillett).” INSECT MOLECULAR BIOLOGY 30 (6): 605–14. https://doi.org/10.1111/imb.12729.
Chicago author-date (all authors)
Li, W-J, D. Wei, H-L Han, Y-J Song, Y. Wang, H-Q Xu, Guy Smagghe, and J-J Wang. 2021. “Lnc94638 Is a Testis-Specific Long Non-Coding RNA Involved in Spermatozoa Formation in Zeugodacus Cucurbitae (Coquillett).” INSECT MOLECULAR BIOLOGY 30 (6): 605–614. doi:10.1111/imb.12729.
Vancouver
1.
Li W-J, Wei D, Han H-L, Song Y-J, Wang Y, Xu H-Q, et al. lnc94638 is a testis-specific long non-coding RNA involved in spermatozoa formation in Zeugodacus cucurbitae (Coquillett). INSECT MOLECULAR BIOLOGY. 2021;30(6):605–14.
IEEE
[1]
W.-J. Li et al., “lnc94638 is a testis-specific long non-coding RNA involved in spermatozoa formation in Zeugodacus cucurbitae (Coquillett),” INSECT MOLECULAR BIOLOGY, vol. 30, no. 6, pp. 605–614, 2021.
@article{8759456,
  abstract     = {{Long non-coding RNAs (lncRNAs) generally display tissue-specific distributions, and testis-specific lncRNAs form the highest proportion of lncRNAs in many species. Here, we presented a detailed analysis of testis-specific lncRNAs in the melon fly, Zeugodacus cucurbitae, a highly destructive insect pest of cucurbitaceous and other related crops. Most testis-specific lncRNAs were found to be long intergenic non-coding RNAs (lincRNA). The size distribution of these lncRNAs ranged between 600 and 1000 nucleotides. Testis-specific lncRNAs that harboured one isoform number and two exons were the most abundant. Compared to other male tissues, the testis had more highly expressed lncRNAs. The quantitative real-time polymerase chain reaction results of 10 randomly selected testis-specific lncRNAs showed expression patterns consistent with RNA-seq data. Further analysis of the most highly expressed testis-specific lncRNA, lnc94638, was undertaken. Fluorescent in situ hybridization assays localized lnc94638 to the apical region of the testis that contains mature spermatozoa. RNA interference-mediated knockdown of lnc94638 expression reduced spermatozoa numbers and impaired the fertility of Z. cucurbitae male. This study provides a catalogue of testis-specific lncRNAs, shows that the testis-specific lnc94638 is involved in spermatogenesis and has the potential to be used for treating male sterility.}},
  author       = {{Li, W-J and Wei, D. and Han, H-L and Song, Y-J and Wang, Y. and Xu, H-Q and Smagghe, Guy and Wang, J-J}},
  issn         = {{0962-1075}},
  journal      = {{INSECT MOLECULAR BIOLOGY}},
  keywords     = {{IDENTIFICATION,EXPRESSION,ROLES,melon fly,RNA-seq,long non-coding RNA,testis-specific,spermatogenesis,fertility}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{605--614}},
  title        = {{lnc94638 is a testis-specific long non-coding RNA involved in spermatozoa formation in Zeugodacus cucurbitae (Coquillett)}},
  url          = {{http://doi.org/10.1111/imb.12729}},
  volume       = {{30}},
  year         = {{2021}},
}
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