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Topolin cytokinins enhanced shoot proliferation, reduced hyperhydricity and altered cytokinin metabolism in Pistacia vera L. seedling explants

Dhekra Abdouli, Lenka Plačková, Karel Doležal, Taoufik Bettaieb and Stefaan Werbrouck (UGent)
(2022) PLANT SCIENCE. 322.
Author
Organization
Abstract
The effect of 10 mu M meta-topolin (mT) and meta-topolin riboside (mTR) on in vitro proliferation and anomalies of Pistacia vera L. were evaluated and compared to that of 6-benzylaminopurine (BA). The highest proliferation rate (15.6) was recorded in the mT-medium, with a value 6 times higher than in BA-medium. Moreover, the lowest percentage of hyperhydric usable shoots (58,9%) and callus weight (46,9%) were found in mTR-treated shoots. Shoot tip as well as leaf necrosis were not influenced by cytokinin (CK) type. Image analysis was used to evaluate photosynthetic efficiency as well as anthocyanin index. Photosynthesis was more efficient with BA and mTR but the higher anthocyanin accumulation in BA-treated shoots suggests more stress. Endogenous CKs and their metabolites were determined in seedlings and, for the first time, the metabolism of exogenous BA, mT and mTR was studied in pistachio. The stimulating effect on cis-zeatin and its riboside and the appearance of BA and traces of ortho-topolin and para-topolin as natural CKs are discussed. The quantitative and qualitative CK metabolite analyses provides some initial clues as to why topolin would be superior to BA in terms of proliferation rate and avoiding hyperhydricity and allowed a better understanding of the effect of exogenous administration of CK.
Keywords
Pistachio, Endogenous cytokinin, meta-topolin riboside, Shoot tip necrosis, IN-VITRO, ENDOGENOUS CYTOKININ, AROMATIC CYTOKININ, WILLIAMS BANANAS, PLANT-TISSUES, TIP NECROSIS, PROFILES, MICROPROPAGATION, IDENTIFICATION, ORGANOGENESIS

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MLA
Abdouli, Dhekra, et al. “Topolin Cytokinins Enhanced Shoot Proliferation, Reduced Hyperhydricity and Altered Cytokinin Metabolism in Pistacia Vera L. Seedling Explants.” PLANT SCIENCE, vol. 322, 2022, doi:10.1016/j.plantsci.2022.111360.
APA
Abdouli, D., Plačková, L., Doležal, K., Bettaieb, T., & Werbrouck, S. (2022). Topolin cytokinins enhanced shoot proliferation, reduced hyperhydricity and altered cytokinin metabolism in Pistacia vera L. seedling explants. PLANT SCIENCE, 322. https://doi.org/10.1016/j.plantsci.2022.111360
Chicago author-date
Abdouli, Dhekra, Lenka Plačková, Karel Doležal, Taoufik Bettaieb, and Stefaan Werbrouck. 2022. “Topolin Cytokinins Enhanced Shoot Proliferation, Reduced Hyperhydricity and Altered Cytokinin Metabolism in Pistacia Vera L. Seedling Explants.” PLANT SCIENCE 322. https://doi.org/10.1016/j.plantsci.2022.111360.
Chicago author-date (all authors)
Abdouli, Dhekra, Lenka Plačková, Karel Doležal, Taoufik Bettaieb, and Stefaan Werbrouck. 2022. “Topolin Cytokinins Enhanced Shoot Proliferation, Reduced Hyperhydricity and Altered Cytokinin Metabolism in Pistacia Vera L. Seedling Explants.” PLANT SCIENCE 322. doi:10.1016/j.plantsci.2022.111360.
Vancouver
1.
Abdouli D, Plačková L, Doležal K, Bettaieb T, Werbrouck S. Topolin cytokinins enhanced shoot proliferation, reduced hyperhydricity and altered cytokinin metabolism in Pistacia vera L. seedling explants. PLANT SCIENCE. 2022;322.
IEEE
[1]
D. Abdouli, L. Plačková, K. Doležal, T. Bettaieb, and S. Werbrouck, “Topolin cytokinins enhanced shoot proliferation, reduced hyperhydricity and altered cytokinin metabolism in Pistacia vera L. seedling explants,” PLANT SCIENCE, vol. 322, 2022.
@article{8758526,
  abstract     = {{The effect of 10 mu M meta-topolin (mT) and meta-topolin riboside (mTR) on in vitro proliferation and anomalies of Pistacia vera L. were evaluated and compared to that of 6-benzylaminopurine (BA). The highest proliferation rate (15.6) was recorded in the mT-medium, with a value 6 times higher than in BA-medium. Moreover, the lowest percentage of hyperhydric usable shoots (58,9%) and callus weight (46,9%) were found in mTR-treated shoots. Shoot tip as well as leaf necrosis were not influenced by cytokinin (CK) type. Image analysis was used to evaluate photosynthetic efficiency as well as anthocyanin index. Photosynthesis was more efficient with BA and mTR but the higher anthocyanin accumulation in BA-treated shoots suggests more stress. Endogenous CKs and their metabolites were determined in seedlings and, for the first time, the metabolism of exogenous BA, mT and mTR was studied in pistachio. The stimulating effect on cis-zeatin and its riboside and the appearance of BA and traces of ortho-topolin and para-topolin as natural CKs are discussed. The quantitative and qualitative CK metabolite analyses provides some initial clues as to why topolin would be superior to BA in terms of proliferation rate and avoiding hyperhydricity and allowed a better understanding of the effect of exogenous administration of CK.}},
  articleno    = {{111360}},
  author       = {{Abdouli, Dhekra and Plačková, Lenka and Doležal, Karel and Bettaieb, Taoufik and Werbrouck, Stefaan}},
  issn         = {{0168-9452}},
  journal      = {{PLANT SCIENCE}},
  keywords     = {{Pistachio,Endogenous cytokinin,meta-topolin riboside,Shoot tip necrosis,IN-VITRO,ENDOGENOUS CYTOKININ,AROMATIC CYTOKININ,WILLIAMS BANANAS,PLANT-TISSUES,TIP NECROSIS,PROFILES,MICROPROPAGATION,IDENTIFICATION,ORGANOGENESIS}},
  language     = {{eng}},
  pages        = {{7}},
  title        = {{Topolin cytokinins enhanced shoot proliferation, reduced hyperhydricity and altered cytokinin metabolism in Pistacia vera L. seedling explants}},
  url          = {{http://doi.org/10.1016/j.plantsci.2022.111360}},
  volume       = {{322}},
  year         = {{2022}},
}
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