Engineering a highly sensitive biosensor for abscisic acid in mammalian cells
- Author
- Seo Woo Kim (UGent) , Kûbra Alci (UGent) , Femke Van Gaever (UGent) , Yasmine Driege (UGent) , Keylla Bicalho (UGent) , Geert Goeminne (UGent) , Claude Libert (UGent) , Alain Goossens (UGent) , Rudi Beyaert (UGent) and Jens Staal (UGent)
- Organization
- Project
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- Biosynthesis and physiological role of endogenous mammalian “phytohormones” in health and disease
- Elucidating the details of the interaction between specific nutrients-microbiota-intestinal health and the consequences for inflammatory diseases
- Engineering of an abscisic acid (ABA)-producing probiotic Saccharomyces boulardii strain to modulate host microbiota, intestinal health and inflammatory diseases
- Abstract
- Abscisic acid (ABA) is a signaling molecule conserved in plants, bacteria, fungi and animals. Recently, ABA has gained attention for its pharmacological activities and its potential as a biomarker for the severity of chronic obstructive pulmonary disease (COPD) and glioma. This prompts the development of a reliable, sensitive, rapid, and cost-effective method to quantify ABA levels in mammalian cells and tissues. The previously described ABA biosensor system based on the ABA-dependent interaction between the plant ABA receptor PYL1 and co-receptor ABI1 is not sensitive enough for the low ABA levels seen in mammals. Therefore, we optimized this system by replacing PYL1 with other high-affinity plant PYL proteins. The optimized biosensor system engineered with the PYL8 receptor enabled the quantification of ABA at low concentrations in HEK293T cells.
- Keywords
- Cell Biology, Genetics, Molecular Biology, Biochemistry, Structural Biology, Biophysics, abscisic acid, biosensor, cell-based assay, synthetic biology, CYCLIC ADP-RIBOSE, PPAR-GAMMA, PROTEIN-2 LANCL2, EXPRESSION, PHYTOHORMONE, INCREASES, DISEASE, ENZYME
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8757253
- MLA
- Kim, Seo Woo, et al. “Engineering a Highly Sensitive Biosensor for Abscisic Acid in Mammalian Cells.” FEBS LETTERS, vol. 596, no. 19, 2022, pp. 2576–90, doi:10.1002/1873-3468.14431.
- APA
- Kim, S. W., Alci, K., Van Gaever, F., Driege, Y., Bicalho, K., Goeminne, G., … Staal, J. (2022). Engineering a highly sensitive biosensor for abscisic acid in mammalian cells. FEBS LETTERS, 596(19), 2576–2590. https://doi.org/10.1002/1873-3468.14431
- Chicago author-date
- Kim, Seo Woo, Kûbra Alci, Femke Van Gaever, Yasmine Driege, Keylla Bicalho, Geert Goeminne, Claude Libert, Alain Goossens, Rudi Beyaert, and Jens Staal. 2022. “Engineering a Highly Sensitive Biosensor for Abscisic Acid in Mammalian Cells.” FEBS LETTERS 596 (19): 2576–90. https://doi.org/10.1002/1873-3468.14431.
- Chicago author-date (all authors)
- Kim, Seo Woo, Kûbra Alci, Femke Van Gaever, Yasmine Driege, Keylla Bicalho, Geert Goeminne, Claude Libert, Alain Goossens, Rudi Beyaert, and Jens Staal. 2022. “Engineering a Highly Sensitive Biosensor for Abscisic Acid in Mammalian Cells.” FEBS LETTERS 596 (19): 2576–2590. doi:10.1002/1873-3468.14431.
- Vancouver
- 1.Kim SW, Alci K, Van Gaever F, Driege Y, Bicalho K, Goeminne G, et al. Engineering a highly sensitive biosensor for abscisic acid in mammalian cells. FEBS LETTERS. 2022;596(19):2576–90.
- IEEE
- [1]S. W. Kim et al., “Engineering a highly sensitive biosensor for abscisic acid in mammalian cells,” FEBS LETTERS, vol. 596, no. 19, pp. 2576–2590, 2022.
@article{8757253,
abstract = {{Abscisic acid (ABA) is a signaling molecule conserved in plants, bacteria, fungi and animals. Recently, ABA has gained attention for its pharmacological activities and its potential as a biomarker for the severity of chronic obstructive pulmonary disease (COPD) and glioma. This prompts the development of a reliable, sensitive, rapid, and cost-effective method to quantify ABA levels in mammalian cells and tissues. The previously described ABA biosensor system based on the ABA-dependent interaction between the plant ABA receptor PYL1 and co-receptor ABI1 is not sensitive enough for the low ABA levels seen in mammals. Therefore, we optimized this system by replacing PYL1 with other high-affinity plant PYL proteins. The optimized biosensor system engineered with the PYL8 receptor enabled the quantification of ABA at low concentrations in HEK293T cells.}},
author = {{Kim, Seo Woo and Alci, Kûbra and Van Gaever, Femke and Driege, Yasmine and Bicalho, Keylla and Goeminne, Geert and Libert, Claude and Goossens, Alain and Beyaert, Rudi and Staal, Jens}},
issn = {{0014-5793}},
journal = {{FEBS LETTERS}},
keywords = {{Cell Biology,Genetics,Molecular Biology,Biochemistry,Structural Biology,Biophysics,abscisic acid,biosensor,cell-based assay,synthetic biology,CYCLIC ADP-RIBOSE,PPAR-GAMMA,PROTEIN-2 LANCL2,EXPRESSION,PHYTOHORMONE,INCREASES,DISEASE,ENZYME}},
language = {{eng}},
number = {{19}},
pages = {{2576--2590}},
title = {{Engineering a highly sensitive biosensor for abscisic acid in mammalian cells}},
url = {{http://dx.doi.org/10.1002/1873-3468.14431}},
volume = {{596}},
year = {{2022}},
}
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