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Engineering a highly sensitive biosensor for abscisic acid in mammalian cells

Seo Woo Kim (UGent) , Kûbra Alci (UGent) , Femke Van Gaever (UGent) , Yasmine Driege (UGent) , Keylla Bicalho (UGent) , Geert Goeminne (UGent) , Claude Libert (UGent) , Alain Goossens (UGent) , Rudi Beyaert (UGent) and Jens Staal (UGent)
(2022) FEBS LETTERS. 596(19). p.2576-2590
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Abstract
Abscisic acid (ABA) is a signaling molecule conserved in plants, bacteria, fungi and animals. Recently, ABA has gained attention for its pharmacological activities and its potential as a biomarker for the severity of chronic obstructive pulmonary disease (COPD) and glioma. This prompts the development of a reliable, sensitive, rapid, and cost-effective method to quantify ABA levels in mammalian cells and tissues. The previously described ABA biosensor system based on the ABA-dependent interaction between the plant ABA receptor PYL1 and co-receptor ABI1 is not sensitive enough for the low ABA levels seen in mammals. Therefore, we optimized this system by replacing PYL1 with other high-affinity plant PYL proteins. The optimized biosensor system engineered with the PYL8 receptor enabled the quantification of ABA at low concentrations in HEK293T cells.
Keywords
Cell Biology, Genetics, Molecular Biology, Biochemistry, Structural Biology, Biophysics, abscisic acid, biosensor, cell-based assay, synthetic biology, CYCLIC ADP-RIBOSE, PPAR-GAMMA, PROTEIN-2 LANCL2, EXPRESSION, PHYTOHORMONE, INCREASES, DISEASE, ENZYME

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Citation

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MLA
Kim, Seo Woo, et al. “Engineering a Highly Sensitive Biosensor for Abscisic Acid in Mammalian Cells.” FEBS LETTERS, vol. 596, no. 19, 2022, pp. 2576–90, doi:10.1002/1873-3468.14431.
APA
Kim, S. W., Alci, K., Van Gaever, F., Driege, Y., Bicalho, K., Goeminne, G., … Staal, J. (2022). Engineering a highly sensitive biosensor for abscisic acid in mammalian cells. FEBS LETTERS, 596(19), 2576–2590. https://doi.org/10.1002/1873-3468.14431
Chicago author-date
Kim, Seo Woo, Kûbra Alci, Femke Van Gaever, Yasmine Driege, Keylla Bicalho, Geert Goeminne, Claude Libert, Alain Goossens, Rudi Beyaert, and Jens Staal. 2022. “Engineering a Highly Sensitive Biosensor for Abscisic Acid in Mammalian Cells.” FEBS LETTERS 596 (19): 2576–90. https://doi.org/10.1002/1873-3468.14431.
Chicago author-date (all authors)
Kim, Seo Woo, Kûbra Alci, Femke Van Gaever, Yasmine Driege, Keylla Bicalho, Geert Goeminne, Claude Libert, Alain Goossens, Rudi Beyaert, and Jens Staal. 2022. “Engineering a Highly Sensitive Biosensor for Abscisic Acid in Mammalian Cells.” FEBS LETTERS 596 (19): 2576–2590. doi:10.1002/1873-3468.14431.
Vancouver
1.
Kim SW, Alci K, Van Gaever F, Driege Y, Bicalho K, Goeminne G, et al. Engineering a highly sensitive biosensor for abscisic acid in mammalian cells. FEBS LETTERS. 2022;596(19):2576–90.
IEEE
[1]
S. W. Kim et al., “Engineering a highly sensitive biosensor for abscisic acid in mammalian cells,” FEBS LETTERS, vol. 596, no. 19, pp. 2576–2590, 2022.
@article{8757253,
  abstract     = {{Abscisic acid (ABA) is a signaling molecule conserved in plants, bacteria, fungi and animals. Recently, ABA has gained attention for its pharmacological activities and its potential as a biomarker for the severity of chronic obstructive pulmonary disease (COPD) and glioma. This prompts the development of a reliable, sensitive, rapid, and cost-effective method to quantify ABA levels in mammalian cells and tissues. The previously described ABA biosensor system based on the ABA-dependent interaction between the plant ABA receptor PYL1 and co-receptor ABI1 is not sensitive enough for the low ABA levels seen in mammals. Therefore, we optimized this system by replacing PYL1 with other high-affinity plant PYL proteins. The optimized biosensor system engineered with the PYL8 receptor enabled the quantification of ABA at low concentrations in HEK293T cells.}},
  author       = {{Kim, Seo Woo and Alci, Kûbra and Van Gaever, Femke and Driege, Yasmine and Bicalho, Keylla and Goeminne, Geert and Libert, Claude and Goossens, Alain and Beyaert, Rudi and Staal, Jens}},
  issn         = {{0014-5793}},
  journal      = {{FEBS LETTERS}},
  keywords     = {{Cell Biology,Genetics,Molecular Biology,Biochemistry,Structural Biology,Biophysics,abscisic acid,biosensor,cell-based assay,synthetic biology,CYCLIC ADP-RIBOSE,PPAR-GAMMA,PROTEIN-2 LANCL2,EXPRESSION,PHYTOHORMONE,INCREASES,DISEASE,ENZYME}},
  language     = {{eng}},
  number       = {{19}},
  pages        = {{2576--2590}},
  title        = {{Engineering a highly sensitive biosensor for abscisic acid in mammalian cells}},
  url          = {{http://doi.org/10.1002/1873-3468.14431}},
  volume       = {{596}},
  year         = {{2022}},
}

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