Academic Bibliography

 Search 200 years of publications by Ghent University researchers.
Advanced search
1 file | 1.88 MB
Add to list
  1. Search results
  2. Optimization and application of a mul...

Optimization and application of a multiplex digital PCR assay for the detection of SARS-CoV-2 variants of concern in Belgian influent wastewater

Tim Boogaerts, Siel Van den Bogaert, Laura A. E. Van Poelvoorde, Diala El Masri, Naomi De Roeck, Nancy H. C. Roosens, Marie Lesenfants, Lies Lahousse (UGent) , Koenraad Van Hoorde, Alexander L. N. van Nuijs, et al.
(2022) VIRUSES-BASEL. 14(3).
Author
Organization
Abstract
Since the beginning of the COVID-19 pandemic, the wastewater-based epidemiology (WBE) of SARS-CoV-2 has been used as a complementary indicator to follow up on the trends in the COVID-19 spread in Belgium and in many other countries. To further develop the use of WBE, a multiplex digital polymerase chain reaction (dPCR) assay was optimized, validated and applied for the measurement of emerging SARS-CoV-2 variants of concern (VOC) in influent wastewater (IWW) samples. Key mutations were targeted in the different VOC strains, including S Delta 69/70 deletion, N501Y, S Delta 241 and S Delta 157. The presented bioanalytical method was able to distinguish between SARS-CoV-2 RNA originating from the wild-type and B.1.1.7, B.1.351 and B.1.617.2 variants. The dPCR assay proved to be sensitive enough to detect low concentrations of SARS-CoV-2 RNA in IWW since the limit of detection of the different targets ranged between 0.3 and 2.9 copies/mu L. This developed WBE approach was applied to IWW samples originating from different Belgian locations and was able to monitor spatio-temporal changes in the presence of targeted VOC strains in the investigated communities. The present dPCR assay developments were realized to bring added-value to the current national WBE of COVID-19 by also having the spatio-temporal proportions of the VoC in presence in the wastewaters.
Keywords
wastewater-based epidemiology, variants of concern, digital polymerase, chain reaction, Belgium, SARS-CoV-2

Downloads

  • Download
    published.pdf
    • full text (Published version)
    • |
    • open access
    • |
    • application/octet-stream
    • |
    • 1.88 MB

Citation

Please use this url to cite or link to this publication:

MLA
Boogaerts, Tim, et al. “Optimization and Application of a Multiplex Digital PCR Assay for the Detection of SARS-CoV-2 Variants of Concern in Belgian Influent Wastewater.” VIRUSES-BASEL, vol. 14, no. 3, 2022, doi:10.3390/v14030610.
APA
Boogaerts, T., Van den Bogaert, S., Van Poelvoorde, L. A. E., El Masri, D., De Roeck, N., Roosens, N. H. C., … Delputte, P. (2022). Optimization and application of a multiplex digital PCR assay for the detection of SARS-CoV-2 variants of concern in Belgian influent wastewater. VIRUSES-BASEL, 14(3). https://doi.org/10.3390/v14030610
Chicago author-date
Boogaerts, Tim, Siel Van den Bogaert, Laura A. E. Van Poelvoorde, Diala El Masri, Naomi De Roeck, Nancy H. C. Roosens, Marie Lesenfants, et al. 2022. “Optimization and Application of a Multiplex Digital PCR Assay for the Detection of SARS-CoV-2 Variants of Concern in Belgian Influent Wastewater.” VIRUSES-BASEL 14 (3). https://doi.org/10.3390/v14030610.
Chicago author-date (all authors)
Boogaerts, Tim, Siel Van den Bogaert, Laura A. E. Van Poelvoorde, Diala El Masri, Naomi De Roeck, Nancy H. C. Roosens, Marie Lesenfants, Lies Lahousse, Koenraad Van Hoorde, Alexander L. N. van Nuijs, and Peter Delputte. 2022. “Optimization and Application of a Multiplex Digital PCR Assay for the Detection of SARS-CoV-2 Variants of Concern in Belgian Influent Wastewater.” VIRUSES-BASEL 14 (3). doi:10.3390/v14030610.
Vancouver
1.
Boogaerts T, Van den Bogaert S, Van Poelvoorde LAE, El Masri D, De Roeck N, Roosens NHC, et al. Optimization and application of a multiplex digital PCR assay for the detection of SARS-CoV-2 variants of concern in Belgian influent wastewater. VIRUSES-BASEL. 2022;14(3).
IEEE
[1]
T. Boogaerts et al., “Optimization and application of a multiplex digital PCR assay for the detection of SARS-CoV-2 variants of concern in Belgian influent wastewater,” VIRUSES-BASEL, vol. 14, no. 3, 2022.
@article{8756155,
  abstract     = {{Since the beginning of the COVID-19 pandemic, the wastewater-based epidemiology (WBE) of SARS-CoV-2 has been used as a complementary indicator to follow up on the trends in the COVID-19 spread in Belgium and in many other countries. To further develop the use of WBE, a multiplex digital polymerase chain reaction (dPCR) assay was optimized, validated and applied for the measurement of emerging SARS-CoV-2 variants of concern (VOC) in influent wastewater (IWW) samples. Key mutations were targeted in the different VOC strains, including S Delta 69/70 deletion, N501Y, S Delta 241 and S Delta 157. The presented bioanalytical method was able to distinguish between SARS-CoV-2 RNA originating from the wild-type and B.1.1.7, B.1.351 and B.1.617.2 variants. The dPCR assay proved to be sensitive enough to detect low concentrations of SARS-CoV-2 RNA in IWW since the limit of detection of the different targets ranged between 0.3 and 2.9 copies/mu L. This developed WBE approach was applied to IWW samples originating from different Belgian locations and was able to monitor spatio-temporal changes in the presence of targeted VOC strains in the investigated communities. The present dPCR assay developments were realized to bring added-value to the current national WBE of COVID-19 by also having the spatio-temporal proportions of the VoC in presence in the wastewaters.}},
  articleno    = {{610}},
  author       = {{Boogaerts, Tim and Van den Bogaert, Siel and Van Poelvoorde, Laura A. E. and El Masri, Diala and De Roeck, Naomi and Roosens, Nancy H. C. and Lesenfants, Marie and Lahousse, Lies and Van Hoorde, Koenraad and van Nuijs, Alexander L. N. and Delputte, Peter}},
  issn         = {{1999-4915}},
  journal      = {{VIRUSES-BASEL}},
  keywords     = {{wastewater-based epidemiology,variants of concern,digital polymerase,chain reaction,Belgium,SARS-CoV-2}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{17}},
  title        = {{Optimization and application of a multiplex digital PCR assay for the detection of SARS-CoV-2 variants of concern in Belgian influent wastewater}},
  url          = {{http://dx.doi.org/10.3390/v14030610}},
  volume       = {{14}},
  year         = {{2022}},
}
Altmetric
View in Altmetric
Web of Science
Times cited: