Light triggered nanoscale biolistics for efficient intracellular delivery of functional macromolecules in mammalian cells
- Author
- Juan Fraire (UGent) , Elnaz Shaabani Sichani, Maryam Sharifiaghdam, Matthias Rombaut, Charlotte Hinnekens (UGent) , Dawei Hua, Jana Ramon (UGent) , Laurens Raes, Eduardo Bolea Fernandez (UGent) , Toon Brans (UGent) , Frank Vanhaecke (UGent) , Peter Borghgraef (UGent) , Chaobo Huang, Félix Sauvage (UGent) , Tamara Vanhaecke, Joery De Kock, Ranhua Xiong (UGent) , Stefaan De Smedt (UGent) and Kevin Braeckmans (UGent)
- Organization
- Project
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- NANOBUBBLE (Laser-induced vapour nanobubbles for intracellular delivery of nanomaterials and treatment of biofilm infections)
- Light-triggered nanobombs for intracellular delivery of functional macromolecules
- Temporally and spatially resolved multi-element information at the micro- and nano-scale: development of new analytical methods and their application to metallomics and nanotechnology
- Abstract
- Biolistic intracellular delivery of functional macromolecules makes use of dense microparticles which are ballistically fired onto cells with a pressurized gun. While it has been used to transfect plant cells, its application to mammalian cells has met with limited success mainly due to high toxicity. Here we present a more refined nanotechnological approach to biolistic delivery with light-triggered self-assembled nanobombs (NBs) that consist of a photothermal core particle surrounded by smaller nanoprojectiles. Upon irradiation with pulsed laser light, fast heating of the core particle results in vapor bubble formation, which propels the nanoprojectiles through the cell membrane of nearby cells. We show successful transfection of both adherent and non-adherent cells with mRNA and pDNA, outperforming electroporation as the most used physical transfection technology by a factor of 5.5-7.6 in transfection yield. With a throughput of 10(4)-10(5) cells per second, biolistic delivery with NBs offers scalable and highly efficient transfections of mammalian cells.
- Keywords
- General Physics and Astronomy, General Biochemistry, Genetics and Molecular Biology, General Chemistry, GENE-TRANSFER, LASER, NANOPARTICLES, TRANSFECTION, OPTOPORATION, MEMBRANE, PLGA
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8755185
- MLA
- Fraire, Juan, et al. “Light Triggered Nanoscale Biolistics for Efficient Intracellular Delivery of Functional Macromolecules in Mammalian Cells.” NATURE COMMUNICATIONS, vol. 13, no. 1, 2022, doi:10.1038/s41467-022-29713-7.
- APA
- Fraire, J., Shaabani Sichani, E., Sharifiaghdam, M., Rombaut, M., Hinnekens, C., Hua, D., … Braeckmans, K. (2022). Light triggered nanoscale biolistics for efficient intracellular delivery of functional macromolecules in mammalian cells. NATURE COMMUNICATIONS, 13(1). https://doi.org/10.1038/s41467-022-29713-7
- Chicago author-date
- Fraire, Juan, Elnaz Shaabani Sichani, Maryam Sharifiaghdam, Matthias Rombaut, Charlotte Hinnekens, Dawei Hua, Jana Ramon, et al. 2022. “Light Triggered Nanoscale Biolistics for Efficient Intracellular Delivery of Functional Macromolecules in Mammalian Cells.” NATURE COMMUNICATIONS 13 (1). https://doi.org/10.1038/s41467-022-29713-7.
- Chicago author-date (all authors)
- Fraire, Juan, Elnaz Shaabani Sichani, Maryam Sharifiaghdam, Matthias Rombaut, Charlotte Hinnekens, Dawei Hua, Jana Ramon, Laurens Raes, Eduardo Bolea Fernandez, Toon Brans, Frank Vanhaecke, Peter Borghgraef, Chaobo Huang, Félix Sauvage, Tamara Vanhaecke, Joery De Kock, Ranhua Xiong, Stefaan De Smedt, and Kevin Braeckmans. 2022. “Light Triggered Nanoscale Biolistics for Efficient Intracellular Delivery of Functional Macromolecules in Mammalian Cells.” NATURE COMMUNICATIONS 13 (1). doi:10.1038/s41467-022-29713-7.
- Vancouver
- 1.Fraire J, Shaabani Sichani E, Sharifiaghdam M, Rombaut M, Hinnekens C, Hua D, et al. Light triggered nanoscale biolistics for efficient intracellular delivery of functional macromolecules in mammalian cells. NATURE COMMUNICATIONS. 2022;13(1).
- IEEE
- [1]J. Fraire et al., “Light triggered nanoscale biolistics for efficient intracellular delivery of functional macromolecules in mammalian cells,” NATURE COMMUNICATIONS, vol. 13, no. 1, 2022.
@article{8755185,
abstract = {{Biolistic intracellular delivery of functional macromolecules makes use of dense microparticles which are ballistically fired onto cells with a pressurized gun. While it has been used to transfect plant cells, its application to mammalian cells has met with limited success mainly due to high toxicity. Here we present a more refined nanotechnological approach to biolistic delivery with light-triggered self-assembled nanobombs (NBs) that consist of a photothermal core particle surrounded by smaller nanoprojectiles. Upon irradiation with pulsed laser light, fast heating of the core particle results in vapor bubble formation, which propels the nanoprojectiles through the cell membrane of nearby cells. We show successful transfection of both adherent and non-adherent cells with mRNA and pDNA, outperforming electroporation as the most used physical transfection technology by a factor of 5.5-7.6 in transfection yield. With a throughput of 10(4)-10(5) cells per second, biolistic delivery with NBs offers scalable and highly efficient transfections of mammalian cells.}},
articleno = {{1996}},
author = {{Fraire, Juan and Shaabani Sichani, Elnaz and Sharifiaghdam, Maryam and Rombaut, Matthias and Hinnekens, Charlotte and Hua, Dawei and Ramon, Jana and Raes, Laurens and Bolea Fernandez, Eduardo and Brans, Toon and Vanhaecke, Frank and Borghgraef, Peter and Huang, Chaobo and Sauvage, Félix and Vanhaecke, Tamara and De Kock, Joery and Xiong, Ranhua and De Smedt, Stefaan and Braeckmans, Kevin}},
issn = {{2041-1723}},
journal = {{NATURE COMMUNICATIONS}},
keywords = {{General Physics and Astronomy,General Biochemistry,Genetics and Molecular Biology,General Chemistry,GENE-TRANSFER,LASER,NANOPARTICLES,TRANSFECTION,OPTOPORATION,MEMBRANE,PLGA}},
language = {{eng}},
number = {{1}},
title = {{Light triggered nanoscale biolistics for efficient intracellular delivery of functional macromolecules in mammalian cells}},
url = {{http://doi.org/10.1038/s41467-022-29713-7}},
volume = {{13}},
year = {{2022}},
}
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