Yeast-produced fructosamine-3-kinase retains mobility after ex vivo intravitreal injection in human and bovine eyes as determined by Fluorescence Correlation Spectroscopy
- Author
- An-Katrien Minnaert (UGent) , Loes van Schie (UGent) , Hendrik Grootaert (UGent) , Jonas Himpe (UGent) , Simon Devos (UGent) , Wannes Weyts (UGent) , Herlinde De Keersmaecker (UGent) , Kevin Braeckmans (UGent) , Elisabeth Van Aken (UGent) , Joris Delanghe (UGent) , Stefaan De Smedt (UGent) , Nico Callewaert (UGent) and Katrien Remaut (UGent)
- Organization
- Project
-
- TREATMENT OF GLYCATED TISSUES BY FRUCTOSAMINE-3 KINASE INJECTION
- The barrier role of the vitreoretinal interface toward intravitreal injected proteins, viruses and siRNA nanoparticles: ex vivo bovine screening models and siRNA nanoparticle design.
- In vitro and in vivo glyco-engineering of human IgG, and the leukocyte-cell surface with new fucosidasen
- Bestrijding van Mycobacterium tuberculosis-infectie met celwanddegraderende enzymen
- Abstract
- Globally, over 2 billion people suffer from vision impairment. Despite complex multifactorial etiology, advanced glycation end products are involved in the pathogenesis of many causative age-and diabetes-related eye diseases. Deglycating enzyme fructosamine-3-kinase (FN3K) was recently proposed as a potential therapeutic, but for further biopharmaceutical development, knowledge on its manufacturability and stability and mobility in the vitreous fluid of the eye is indispensable. We evaluated recombinant production of FN3K in two host systems, and its diffusion behavior in both bovine and human vitreous. Compared to Escherichia coli, intracellular production in Pichia pastoris yielded more and higher purity FN3K. The yeast-produced enzyme was used in a first attempt to use fluorescence correlation spectroscopy to study protein mobility in non-sonicated bovine vitreous, human vitreous, and intact bovine eyes. It was demonstrated that FN3K retained mobility upon intravitreal injection, although a certain delay in diffusion was observed. Alkylation of free cysteines was tolerated both in terms of enzymatic activity and vitreous diffusion. Ex vivo diffusion data gathered and the availability of yeast-produced high purity enzyme now clear the path for in vivo pharmacokinetics studies of FN3K.
- Keywords
- Pharmaceutical Science, Fructosamine-3-kinase (FN3K), Fluorescence correlation spectroscopy (FCS), Vitreous, Cataract, Age-related macular degeneration (AMD), Pichia pastoris, GLYCATION END-PRODUCTS, PROTEIN AGGREGATION, VITREOUS-HUMOR, DIFFUSION, PHARMACOKINETICS, VISCOELASTICITY, RHEOLOGY, RABBIT, MODEL, BODY
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8754766
- MLA
- Minnaert, An-Katrien, et al. “Yeast-Produced Fructosamine-3-Kinase Retains Mobility after Ex Vivo Intravitreal Injection in Human and Bovine Eyes as Determined by Fluorescence Correlation Spectroscopy.” INTERNATIONAL JOURNAL OF PHARMACEUTICS, vol. 621, 2022, doi:10.1016/j.ijpharm.2022.121772.
- APA
- Minnaert, A.-K., van Schie, L., Grootaert, H., Himpe, J., Devos, S., Weyts, W., … Remaut, K. (2022). Yeast-produced fructosamine-3-kinase retains mobility after ex vivo intravitreal injection in human and bovine eyes as determined by Fluorescence Correlation Spectroscopy. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 621. https://doi.org/10.1016/j.ijpharm.2022.121772
- Chicago author-date
- Minnaert, An-Katrien, Loes van Schie, Hendrik Grootaert, Jonas Himpe, Simon Devos, Wannes Weyts, Herlinde De Keersmaecker, et al. 2022. “Yeast-Produced Fructosamine-3-Kinase Retains Mobility after Ex Vivo Intravitreal Injection in Human and Bovine Eyes as Determined by Fluorescence Correlation Spectroscopy.” INTERNATIONAL JOURNAL OF PHARMACEUTICS 621. https://doi.org/10.1016/j.ijpharm.2022.121772.
- Chicago author-date (all authors)
- Minnaert, An-Katrien, Loes van Schie, Hendrik Grootaert, Jonas Himpe, Simon Devos, Wannes Weyts, Herlinde De Keersmaecker, Kevin Braeckmans, Elisabeth Van Aken, Joris Delanghe, Stefaan De Smedt, Nico Callewaert, and Katrien Remaut. 2022. “Yeast-Produced Fructosamine-3-Kinase Retains Mobility after Ex Vivo Intravitreal Injection in Human and Bovine Eyes as Determined by Fluorescence Correlation Spectroscopy.” INTERNATIONAL JOURNAL OF PHARMACEUTICS 621. doi:10.1016/j.ijpharm.2022.121772.
- Vancouver
- 1.Minnaert A-K, van Schie L, Grootaert H, Himpe J, Devos S, Weyts W, et al. Yeast-produced fructosamine-3-kinase retains mobility after ex vivo intravitreal injection in human and bovine eyes as determined by Fluorescence Correlation Spectroscopy. INTERNATIONAL JOURNAL OF PHARMACEUTICS. 2022;621.
- IEEE
- [1]A.-K. Minnaert et al., “Yeast-produced fructosamine-3-kinase retains mobility after ex vivo intravitreal injection in human and bovine eyes as determined by Fluorescence Correlation Spectroscopy,” INTERNATIONAL JOURNAL OF PHARMACEUTICS, vol. 621, 2022.
@article{8754766,
abstract = {{Globally, over 2 billion people suffer from vision impairment. Despite complex multifactorial etiology, advanced glycation end products are involved in the pathogenesis of many causative age-and diabetes-related eye diseases. Deglycating enzyme fructosamine-3-kinase (FN3K) was recently proposed as a potential therapeutic, but for further biopharmaceutical development, knowledge on its manufacturability and stability and mobility in the vitreous fluid of the eye is indispensable. We evaluated recombinant production of FN3K in two host systems, and its diffusion behavior in both bovine and human vitreous. Compared to Escherichia coli, intracellular production in Pichia pastoris yielded more and higher purity FN3K. The yeast-produced enzyme was used in a first attempt to use fluorescence correlation spectroscopy to study protein mobility in non-sonicated bovine vitreous, human vitreous, and intact bovine eyes. It was demonstrated that FN3K retained mobility upon intravitreal injection, although a certain delay in diffusion was observed. Alkylation of free cysteines was tolerated both in terms of enzymatic activity and vitreous diffusion. Ex vivo diffusion data gathered and the availability of yeast-produced high purity enzyme now clear the path for in vivo pharmacokinetics studies of FN3K.}},
articleno = {{121772}},
author = {{Minnaert, An-Katrien and van Schie, Loes and Grootaert, Hendrik and Himpe, Jonas and Devos, Simon and Weyts, Wannes and De Keersmaecker, Herlinde and Braeckmans, Kevin and Van Aken, Elisabeth and Delanghe, Joris and De Smedt, Stefaan and Callewaert, Nico and Remaut, Katrien}},
issn = {{0378-5173}},
journal = {{INTERNATIONAL JOURNAL OF PHARMACEUTICS}},
keywords = {{Pharmaceutical Science,Fructosamine-3-kinase (FN3K),Fluorescence correlation spectroscopy (FCS),Vitreous,Cataract,Age-related macular degeneration (AMD),Pichia pastoris,GLYCATION END-PRODUCTS,PROTEIN AGGREGATION,VITREOUS-HUMOR,DIFFUSION,PHARMACOKINETICS,VISCOELASTICITY,RHEOLOGY,RABBIT,MODEL,BODY}},
language = {{eng}},
pages = {{13}},
title = {{Yeast-produced fructosamine-3-kinase retains mobility after ex vivo intravitreal injection in human and bovine eyes as determined by Fluorescence Correlation Spectroscopy}},
url = {{http://doi.org/10.1016/j.ijpharm.2022.121772}},
volume = {{621}},
year = {{2022}},
}
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