Advanced search
1 file | 5.54 MB Add to list

Exploring the transportome of the biosurfactant producing yeast Starmerella bombicola

(2022) BMC GENOMICS. 23(1).
Author
Organization
Abstract
Starmerella bombicola is a non-conventional yeast mainly known for its capacity to produce high amounts of the glycolipids 'sophorolipids'. Although its product has been used as biological detergent for a couple of decades, the genetics of S. bombicola are still largely unknown. Computational analysis of the yeast's genome enabled us to identify 254 putative transporter genes that make up the entire transportome. For each of them, a potential substrate was predicted using homology analysis, subcellular localization prediction and RNA sequencing in different stages of growth. One transporter family is of exceptional importance to this yeast: the ATP Binding Cassette (ABC) transporter Superfamily, because it harbors the main driver behind the highly efficient sophorolipid export. Furthermore, members of this superfamily translocate a variety of compounds ranging from antibiotics to hydrophobic molecules. We conducted an analysis of this family by creating deletion mutants to understand their role in the export of hydrophobic compounds, antibiotics and sophorolipids. Doing this, we could experimentally confirm the transporters participating in the efflux of medium chain fatty alcohols, particularly decanol and undecanol, and identify a second sophorolipid transporter that is located outside the sophorolipid biosynthetic gene cluster.
Keywords
Starmerella bombicola, Transportome, Sophorolipid, Yeast, ABC transporters, SACCHAROMYCES-CEREVISIAE, ABC TRANSPORTERS, GENE-EXPRESSION, TRANSFORMATION, SOPHOROLIPIDS, INHIBITION, SECRETION, NITROGEN, DATABASE, SYSTEMS

Downloads

  • s12864-021-08177-x.pdf
    • full text (Published version)
    • |
    • open access
    • |
    • PDF
    • |
    • 5.54 MB

Citation

Please use this url to cite or link to this publication:

MLA
Claus, Silke, et al. “Exploring the Transportome of the Biosurfactant Producing Yeast Starmerella Bombicola.” BMC GENOMICS, vol. 23, no. 1, 2022, doi:10.1186/s12864-021-08177-x.
APA
Claus, S., Jezierska, S., Elbourne, L. D. H., & Van Bogaert, I. (2022). Exploring the transportome of the biosurfactant producing yeast Starmerella bombicola. BMC GENOMICS, 23(1). https://doi.org/10.1186/s12864-021-08177-x
Chicago author-date
Claus, Silke, Sylwia Jezierska, Liam D. H. Elbourne, and Inge Van Bogaert. 2022. “Exploring the Transportome of the Biosurfactant Producing Yeast Starmerella Bombicola.” BMC GENOMICS 23 (1). https://doi.org/10.1186/s12864-021-08177-x.
Chicago author-date (all authors)
Claus, Silke, Sylwia Jezierska, Liam D. H. Elbourne, and Inge Van Bogaert. 2022. “Exploring the Transportome of the Biosurfactant Producing Yeast Starmerella Bombicola.” BMC GENOMICS 23 (1). doi:10.1186/s12864-021-08177-x.
Vancouver
1.
Claus S, Jezierska S, Elbourne LDH, Van Bogaert I. Exploring the transportome of the biosurfactant producing yeast Starmerella bombicola. BMC GENOMICS. 2022;23(1).
IEEE
[1]
S. Claus, S. Jezierska, L. D. H. Elbourne, and I. Van Bogaert, “Exploring the transportome of the biosurfactant producing yeast Starmerella bombicola,” BMC GENOMICS, vol. 23, no. 1, 2022.
@article{8754081,
  abstract     = {{Starmerella bombicola is a non-conventional yeast mainly known for its capacity to produce high amounts of the glycolipids 'sophorolipids'. Although its product has been used as biological detergent for a couple of decades, the genetics of S. bombicola are still largely unknown. Computational analysis of the yeast's genome enabled us to identify 254 putative transporter genes that make up the entire transportome. For each of them, a potential substrate was predicted using homology analysis, subcellular localization prediction and RNA sequencing in different stages of growth. One transporter family is of exceptional importance to this yeast: the ATP Binding Cassette (ABC) transporter Superfamily, because it harbors the main driver behind the highly efficient sophorolipid export. Furthermore, members of this superfamily translocate a variety of compounds ranging from antibiotics to hydrophobic molecules. We conducted an analysis of this family by creating deletion mutants to understand their role in the export of hydrophobic compounds, antibiotics and sophorolipids. Doing this, we could experimentally confirm the transporters participating in the efflux of medium chain fatty alcohols, particularly decanol and undecanol, and identify a second sophorolipid transporter that is located outside the sophorolipid biosynthetic gene cluster.}},
  articleno    = {{22}},
  author       = {{Claus, Silke and Jezierska, Sylwia and Elbourne, Liam D. H. and Van Bogaert, Inge}},
  issn         = {{1471-2164}},
  journal      = {{BMC GENOMICS}},
  keywords     = {{Starmerella bombicola,Transportome,Sophorolipid,Yeast,ABC transporters,SACCHAROMYCES-CEREVISIAE,ABC TRANSPORTERS,GENE-EXPRESSION,TRANSFORMATION,SOPHOROLIPIDS,INHIBITION,SECRETION,NITROGEN,DATABASE,SYSTEMS}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{17}},
  title        = {{Exploring the transportome of the biosurfactant producing yeast Starmerella bombicola}},
  url          = {{http://doi.org/10.1186/s12864-021-08177-x}},
  volume       = {{23}},
  year         = {{2022}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: