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Force in numbers : high-throughput screening approaches to unlock microbial transport

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Abstract
Biological membranes are inherently complex, making transport processes in microbial cell factories a significant bottleneck. Lack of knowledge on transport proteins' characteristics and the need for advanced technical equipment often hamper transporter identification and optimization. For these reasons, moving away from individual characterization and towards high-throughput mining, engineering, and screening of transporters is an increasingly attractive approach. Superior transporters can be selected from large libraries by coupling their activity to growth, for substrates that function as feedstocks or toxic compounds. Other compounds can be screened thanks to recent advances in the design and deployment of synthetic genetic circuits (biosensors). Furthermore, novel strategies are rapidly increasing the repertoire of biomolecule transporters susceptible to high-throughput selection methods.
Keywords
DIRECTED EVOLUTION, SACCHAROMYCES-CEREVISIAE, ESCHERICHIA-COLI, ACID TRANSPORTER, EFFLUX PUMP, BIOSENSORS, TOLERANCE, DESIGN, YEAST

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MLA
Jenkins Sánchez, Liam Richard, et al. “Force in Numbers : High-Throughput Screening Approaches to Unlock Microbial Transport.” CURRENT OPINION IN BIOTECHNOLOGY, vol. 74, 2022, pp. 204–10, doi:10.1016/j.copbio.2021.11.012.
APA
Jenkins Sánchez, L. R., Claus, S., Muth, L. T., Salvador López, J. M., & Van Bogaert, I. (2022). Force in numbers : high-throughput screening approaches to unlock microbial transport. CURRENT OPINION IN BIOTECHNOLOGY, 74, 204–210. https://doi.org/10.1016/j.copbio.2021.11.012
Chicago author-date
Jenkins Sánchez, Liam Richard, Silke Claus, Liv Teresa Muth, José Manuel Salvador López, and Inge Van Bogaert. 2022. “Force in Numbers : High-Throughput Screening Approaches to Unlock Microbial Transport.” CURRENT OPINION IN BIOTECHNOLOGY 74: 204–10. https://doi.org/10.1016/j.copbio.2021.11.012.
Chicago author-date (all authors)
Jenkins Sánchez, Liam Richard, Silke Claus, Liv Teresa Muth, José Manuel Salvador López, and Inge Van Bogaert. 2022. “Force in Numbers : High-Throughput Screening Approaches to Unlock Microbial Transport.” CURRENT OPINION IN BIOTECHNOLOGY 74: 204–210. doi:10.1016/j.copbio.2021.11.012.
Vancouver
1.
Jenkins Sánchez LR, Claus S, Muth LT, Salvador López JM, Van Bogaert I. Force in numbers : high-throughput screening approaches to unlock microbial transport. CURRENT OPINION IN BIOTECHNOLOGY. 2022;74:204–10.
IEEE
[1]
L. R. Jenkins Sánchez, S. Claus, L. T. Muth, J. M. Salvador López, and I. Van Bogaert, “Force in numbers : high-throughput screening approaches to unlock microbial transport,” CURRENT OPINION IN BIOTECHNOLOGY, vol. 74, pp. 204–210, 2022.
@article{8754079,
  abstract     = {{Biological membranes are inherently complex, making transport processes in microbial cell factories a significant bottleneck. Lack of knowledge on transport proteins' characteristics and the need for advanced technical equipment often hamper transporter identification and optimization. For these reasons, moving away from individual characterization and towards high-throughput mining, engineering, and screening of transporters is an increasingly attractive approach. Superior transporters can be selected from large libraries by coupling their activity to growth, for substrates that function as feedstocks or toxic compounds. Other compounds can be screened thanks to recent advances in the design and deployment of synthetic genetic circuits (biosensors). Furthermore, novel strategies are rapidly increasing the repertoire of biomolecule transporters susceptible to high-throughput selection methods.}},
  author       = {{Jenkins Sánchez, Liam Richard and Claus, Silke and Muth, Liv Teresa and Salvador López, José Manuel and Van Bogaert, Inge}},
  issn         = {{0958-1669}},
  journal      = {{CURRENT OPINION IN BIOTECHNOLOGY}},
  keywords     = {{DIRECTED EVOLUTION,SACCHAROMYCES-CEREVISIAE,ESCHERICHIA-COLI,ACID TRANSPORTER,EFFLUX PUMP,BIOSENSORS,TOLERANCE,DESIGN,YEAST}},
  language     = {{eng}},
  pages        = {{204--210}},
  title        = {{Force in numbers : high-throughput screening approaches to unlock microbial transport}},
  url          = {{http://dx.doi.org/10.1016/j.copbio.2021.11.012}},
  volume       = {{74}},
  year         = {{2022}},
}

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