
Improved production of novel (bola) glycolipid biosurfactants with the yeast Starmerella bombicola through an integrative approach combining genetic engineering and multiomics analyses
- Author
- Martijn Castelein (UGent) , Nicolas de Fooz (UGent) , Goedele Luyten (UGent) , Lisa Van Renterghem, Sven Dierickx, Stijn Bovijn (UGent) , Sophie Roelants (UGent) , Lynn Vanhaecke (UGent) and Wim Soetaert (UGent)
- Organization
- Project
- Abstract
- Starmerella bombicola is well known for its natural efficient capacity to produce glycolipids sophorolipids (SLs) at high titers (> 200 g/L) and high-volumetric productivities (> 2 g/L h). These traits make this nonpathogenic yeast industrially relevant, resulting in valorization of SLs as microbial biosurfactants by several companies. The efficient glycolipid machinery of S. bombicola has, on the other hand, been employed toward the biosynthesis of novel types of glycolipid biosurfactants. This has resulted in the generation of a battery of novel S. bombicola strains, producing a range of (novel) glycolipids of which an overview is given in the first part of this chapter. The novel glycolipids have varying properties resulting in a diverse valorization potential, which is very promising toward the further development of the bio-based economy. However, some of the novel S. bombicola strains suffer(ed) from decreased glycolipid productivity levels, while the molecular regulation of glycolipid biosynthesis with S. bombicola is yet to be fully elucidated. Our lab has applied several (integrated) -omic strategies to overcome these hurdles, and in some cases, this strategy allowed us to acquire the information required to resolve the productivity decrease, giving rise to efficient production of novel glycolipids. Further determination and integration of -omic datasets and even in-line/in-process determination of specific -omic data is expected to positively impact further developments and follow-up of glycolipid biosurfactant production processes with S. bombicola.
- Keywords
- Glycolipids, microbial biosurfactants, genetic engineering, multi-omics, proteomics, metabolomics, genomics, transcriptomics, yeast, Starmerella bombicola, multiomics
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8753941
- MLA
- Castelein, Martijn, et al. “Improved Production of Novel (Bola) Glycolipid Biosurfactants with the Yeast Starmerella Bombicola through an Integrative Approach Combining Genetic Engineering and Multiomics Analyses.” Biosurfactants : Research and Development, edited by Gloria Soberón-Chávez, Academic Press, 2023, pp. 183–202, doi:10.1016/B978-0-323-91697-4.00009-0.
- APA
- Castelein, M., de Fooz, N., Luyten, G., Van Renterghem, L., Dierickx, S., Bovijn, S., … Soetaert, W. (2023). Improved production of novel (bola) glycolipid biosurfactants with the yeast Starmerella bombicola through an integrative approach combining genetic engineering and multiomics analyses. In G. Soberón-Chávez (Ed.), Biosurfactants : research and development (pp. 183–202). https://doi.org/10.1016/B978-0-323-91697-4.00009-0
- Chicago author-date
- Castelein, Martijn, Nicolas de Fooz, Goedele Luyten, Lisa Van Renterghem, Sven Dierickx, Stijn Bovijn, Sophie Roelants, Lynn Vanhaecke, and Wim Soetaert. 2023. “Improved Production of Novel (Bola) Glycolipid Biosurfactants with the Yeast Starmerella Bombicola through an Integrative Approach Combining Genetic Engineering and Multiomics Analyses.” In Biosurfactants : Research and Development, edited by Gloria Soberón-Chávez, 183–202. Academic Press. https://doi.org/10.1016/B978-0-323-91697-4.00009-0.
- Chicago author-date (all authors)
- Castelein, Martijn, Nicolas de Fooz, Goedele Luyten, Lisa Van Renterghem, Sven Dierickx, Stijn Bovijn, Sophie Roelants, Lynn Vanhaecke, and Wim Soetaert. 2023. “Improved Production of Novel (Bola) Glycolipid Biosurfactants with the Yeast Starmerella Bombicola through an Integrative Approach Combining Genetic Engineering and Multiomics Analyses.” In Biosurfactants : Research and Development, ed by. Gloria Soberón-Chávez, 183–202. Academic Press. doi:10.1016/B978-0-323-91697-4.00009-0.
- Vancouver
- 1.Castelein M, de Fooz N, Luyten G, Van Renterghem L, Dierickx S, Bovijn S, et al. Improved production of novel (bola) glycolipid biosurfactants with the yeast Starmerella bombicola through an integrative approach combining genetic engineering and multiomics analyses. In: Soberón-Chávez G, editor. Biosurfactants : research and development. Academic Press; 2023. p. 183–202.
- IEEE
- [1]M. Castelein et al., “Improved production of novel (bola) glycolipid biosurfactants with the yeast Starmerella bombicola through an integrative approach combining genetic engineering and multiomics analyses,” in Biosurfactants : research and development, G. Soberón-Chávez, Ed. Academic Press, 2023, pp. 183–202.
@incollection{8753941, abstract = {{Starmerella bombicola is well known for its natural efficient capacity to produce glycolipids sophorolipids (SLs) at high titers (> 200 g/L) and high-volumetric productivities (> 2 g/L h). These traits make this nonpathogenic yeast industrially relevant, resulting in valorization of SLs as microbial biosurfactants by several companies. The efficient glycolipid machinery of S. bombicola has, on the other hand, been employed toward the biosynthesis of novel types of glycolipid biosurfactants. This has resulted in the generation of a battery of novel S. bombicola strains, producing a range of (novel) glycolipids of which an overview is given in the first part of this chapter. The novel glycolipids have varying properties resulting in a diverse valorization potential, which is very promising toward the further development of the bio-based economy. However, some of the novel S. bombicola strains suffer(ed) from decreased glycolipid productivity levels, while the molecular regulation of glycolipid biosynthesis with S. bombicola is yet to be fully elucidated. Our lab has applied several (integrated) -omic strategies to overcome these hurdles, and in some cases, this strategy allowed us to acquire the information required to resolve the productivity decrease, giving rise to efficient production of novel glycolipids. Further determination and integration of -omic datasets and even in-line/in-process determination of specific -omic data is expected to positively impact further developments and follow-up of glycolipid biosurfactant production processes with S. bombicola.}}, author = {{Castelein, Martijn and de Fooz, Nicolas and Luyten, Goedele and Van Renterghem, Lisa and Dierickx, Sven and Bovijn, Stijn and Roelants, Sophie and Vanhaecke, Lynn and Soetaert, Wim}}, booktitle = {{Biosurfactants : research and development}}, editor = {{Soberón-Chávez, Gloria}}, isbn = {{9780323916974}}, keywords = {{Glycolipids,microbial biosurfactants,genetic engineering,multi-omics,proteomics,metabolomics,genomics,transcriptomics,yeast,Starmerella bombicola,multiomics}}, language = {{eng}}, pages = {{183--202}}, publisher = {{Academic Press}}, series = {{Foundations and Frontiers in Enzymology}}, title = {{Improved production of novel (bola) glycolipid biosurfactants with the yeast Starmerella bombicola through an integrative approach combining genetic engineering and multiomics analyses}}, url = {{http://doi.org/10.1016/B978-0-323-91697-4.00009-0}}, year = {{2023}}, }
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