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Modulation of the DA1 pathway in maize shows that translatability of information from Arabidopsis to crops is complex

Pan Gong (UGent) , Kirin Demuynck (UGent) , Jolien De Block (UGent) , Stijn Aesaert (UGent) , Griet Coussens (UGent) , Laurens Pauwels (UGent) , Dirk Inzé (UGent) and Hilde Nelissen (UGent)
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Abstract
Modern agriculture is struggling to meet the increasing food, silage and raw material demands due to the rapid growth of population and climate change. In Arabidopsis, DA1 and DAR1 are proteases that negatively regulate cell proliferation and control organ size. DA1 and DAR1 are activated by ubiquitination catalyzed by the E3 ligase BIG BROTHER (BB). Here, we characterized the DA1, DAR1 and BB gene families in maize and analyzed whether perturbation of these genes regulates organ size similar to what was observed in Arabidopsis. We generated da1_dar1a_dar1b triple CRISPR maize mutants and bb1_bb2 double mutants. Detailed phenotypic analysis showed that the size of leaf, stem, cob, and seed was not consistently enlarged in these mutants. Also overexpression of a dominant-negative DA1R333K allele, resembling the da1-1 allele of Arabidopsis which has larger leaves and seeds, did not alter the maize phenotype. The mild negative effects on plant height of the DA1R333K_bb1_bb2 mutant indicate that the genes in the DA1 pathway may control organ size in maize, albeit less obvious than in Arabidopsis.
Keywords
maize growthCRISPR/Cas9DA1DAR1BIG BROTHER

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Citation

Please use this url to cite or link to this publication:

MLA
Gong, Pan, et al. “Modulation of the DA1 Pathway in Maize Shows That Translatability of Information from Arabidopsis to Crops Is Complex.” Plant Science, vol. 312, 2022, doi:10.1016/j.plantsci.2022.111295.
APA
Gong, P., Demuynck, K., De Block, J., Aesaert, S., Coussens, G., Pauwels, L., … Nelissen, H. (2022). Modulation of the DA1 pathway in maize shows that translatability of information from Arabidopsis to crops is complex. Plant Science, 312. https://doi.org/10.1016/j.plantsci.2022.111295
Chicago author-date
Gong, Pan, Kirin Demuynck, Jolien De Block, Stijn Aesaert, Griet Coussens, Laurens Pauwels, Dirk Inzé, and Hilde Nelissen. 2022. “Modulation of the DA1 Pathway in Maize Shows That Translatability of Information from Arabidopsis to Crops Is Complex.” Plant Science 312. https://doi.org/10.1016/j.plantsci.2022.111295.
Chicago author-date (all authors)
Gong, Pan, Kirin Demuynck, Jolien De Block, Stijn Aesaert, Griet Coussens, Laurens Pauwels, Dirk Inzé, and Hilde Nelissen. 2022. “Modulation of the DA1 Pathway in Maize Shows That Translatability of Information from Arabidopsis to Crops Is Complex.” Plant Science 312. doi:10.1016/j.plantsci.2022.111295.
Vancouver
1.
Gong P, Demuynck K, De Block J, Aesaert S, Coussens G, Pauwels L, et al. Modulation of the DA1 pathway in maize shows that translatability of information from Arabidopsis to crops is complex. Plant Science. 2022;312.
IEEE
[1]
P. Gong et al., “Modulation of the DA1 pathway in maize shows that translatability of information from Arabidopsis to crops is complex,” Plant Science, vol. 312, 2022.
@article{8751751,
  abstract     = {{Modern agriculture is struggling to meet the increasing food, silage and raw material demands due to the rapid growth of population and climate change. In Arabidopsis, DA1 and DAR1 are proteases that negatively regulate cell proliferation and control organ size. DA1 and DAR1 are activated by ubiquitination catalyzed by the E3 ligase BIG BROTHER (BB). Here, we characterized the DA1, DAR1 and BB gene families in maize and analyzed whether perturbation of these genes regulates organ size similar to what was observed in Arabidopsis. We generated da1_dar1a_dar1b triple CRISPR maize mutants and bb1_bb2 double mutants. Detailed phenotypic analysis showed that the size of leaf, stem, cob, and seed was not consistently enlarged in these mutants. Also overexpression of a dominant-negative DA1R333K allele, resembling the da1-1 allele of Arabidopsis which has larger leaves and seeds, did not alter the maize phenotype. The mild negative effects on plant height of the DA1R333K_bb1_bb2 mutant indicate that the genes in the DA1 pathway may control organ size in maize, albeit less obvious than in Arabidopsis.}},
  articleno    = {{111295}},
  author       = {{Gong, Pan and Demuynck, Kirin and De Block, Jolien and Aesaert, Stijn and Coussens, Griet and Pauwels, Laurens and Inzé, Dirk and Nelissen, Hilde}},
  issn         = {{0168-9452}},
  journal      = {{Plant Science}},
  keywords     = {{maize growthCRISPR/Cas9DA1DAR1BIG BROTHER}},
  language     = {{eng}},
  title        = {{Modulation of the DA1 pathway in maize shows that translatability of information from Arabidopsis to crops is complex}},
  url          = {{http://dx.doi.org/10.1016/j.plantsci.2022.111295}},
  volume       = {{312}},
  year         = {{2022}},
}

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