@phdthesis{8750973,
  abstract     = {{Since the industrial revolution, manual labour began to be replaced by machinery. Hereby, the accompanied use of fuels impacts the environment in a negative way and led to global warming and climate change. To tackle these global challenges we face today, a shift from a fossil-based economy towards a green, carbon-free, bio-based economy is needed. Hereby, biotechnology is an important player. Industrial biotechnology uses enzymes and microorganisms, such as yeast, to produce biobased products based on renewable resources. However, the development of industrially relevant production strains remains a challenge which is often hampered by imbalances in the production pathway and the metabolism of the host. Indeed, finding the metabolic sweet spot in a (production) pathway is the challenge we face today.

In this Ph.D. dissertation, post-transcriptional regulation to control gene expression was explored. Regulatory elements that influence post-transcriptional regulation were screened and important fundaments were laid for a high-throughput tool that can indicate which post-transcriptional level of gene expression is influenced. In a second part of this Ph.D., different strategies to balance the production pathway of two human milk oligosaccharides were evaluated with the goal to find the metabolic sweet spot of these pathways. Both a rational engineering strategy and a machine-learning approach were applied to do so. In a next step, both parts of this Ph.D. can be brought together, using post-transcriptional regulation for pathway balancing.}},
  author       = {{De Nijs, Yatti}},
  isbn         = {{9789463575089}},
  keywords     = {{synthetic biology,metabolic engineering,Saccharomyces cerevisiae,post-transcriptional regulation,5'UTR,pathway balancing}},
  language     = {{eng}},
  pages        = {{XII, 269}},
  publisher    = {{Ghent University. Faculty of Bioscience Engineering}},
  school       = {{Ghent University}},
  title        = {{Finding the metabolic sweet spot : exploring post-transcriptional regulation and pathway balancing in yeast}},
  year         = {{2022}},
}