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Influence of single layer centrifugation with canicoll on semen freezability in dogs

(2022) ANIMALS. 12(6).
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Abstract
Simple Summary Freezing dog semen is not always possible due to low quality sperm or poor survival during freezing. In order to make this assisted reproductive technique available to a larger number of dogs, this study investigated the benefit of selecting the best spermatozoa before freezing using single layer centrifugation (SLC). The results indicated that this technique was effective in separating spermatozoa according to their quality, although this resulted in losing some good quality spermatozoa. After thawing, spermatozoa centrifuged by SLC were of better quality than after standard centrifugation. However, spermatozoa from suboptimal quality semen did not survive freezing as well as spermatozoa from semen of optimal quality, even after SLC. Single layer centrifugation, therefore, makes it possible to obtain better quality spermatozoa after thawing but is not sufficient on its own to improve the inferior freezing ability of spermatozoa from suboptimal quality semen. So far, eighteen pups were born after insemination with SLC-selected frozen-thawed semen, proving that these selected spermatozoa remain fertile. This study evaluated how semen selection by single layer centrifugation (SLC) with Canicoll affects semen freezability in dogs. A total of eighteen ejaculates, collected from dogs with optimal and suboptimal semen quality (optimal: normal morphology (NM) >= 80%, n = 9; suboptimal: NM between 60 and 79%, n = 9), were divided into two aliquots and subjected to standard centrifugation or SLC before cryopreservation. Motility, NM, membrane integrity, mitochondrial membrane potential (MMP), and DNA integrity were improved in fresh samples after SLC, regardless of semen quality, but at the expense of some good quality spermatozoa. After thawing, NM and membrane integrity were improved in SLC-selected semen in both semen qualities. Interestingly, MMP was also higher but only in optimal quality semen. Still, spermatozoa from suboptimal quality semen did not survive freezing to the same extent as spermatozoa from optimal quality semen, even after selecting superior spermatozoa. Semen selection with Canicoll is, therefore, an effective technique to isolate a subpopulation of high-quality spermatozoa and obtain sperm samples of better quality after thawing, but is not sufficient to improve the intrinsic inferior freezability of suboptimal quality semen. So far, eighteen pups were born after insemination with SLC-selected frozen-thawed semen, proving that these selected spermatozoa remain fertile.
Keywords
SPERM DNA FRAGMENTATION, COLLOID CENTRIFUGATION, LIPID-PEROXIDATION, GENETIC DIVERSITY, OXIDATIVE STRESS, STALLION SEMEN, FROZEN, CRYOPRESERVATION, QUALITY, INSEMINATION, canine, fertility, semen selection, cryopreservation, semen quality

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MLA
Domain, Guillaume, et al. “Influence of Single Layer Centrifugation with Canicoll on Semen Freezability in Dogs.” ANIMALS, vol. 12, no. 6, 2022, doi:10.3390/ani12060714.
APA
Domain, G., Ali Hassan, H., Wydooghe, E., Bogado Pascottini, O. A., Johannisson, A., Morrell, J. M., … Van Soom, A. (2022). Influence of single layer centrifugation with canicoll on semen freezability in dogs. ANIMALS, 12(6). https://doi.org/10.3390/ani12060714
Chicago author-date
Domain, Guillaume, Hiba Ali Hassan, Eline Wydooghe, Osvaldo Américo Bogado Pascottini, Anders Johannisson, Jane M. Morrell, Wojciech Nizanski, and Ann Van Soom. 2022. “Influence of Single Layer Centrifugation with Canicoll on Semen Freezability in Dogs.” ANIMALS 12 (6). https://doi.org/10.3390/ani12060714.
Chicago author-date (all authors)
Domain, Guillaume, Hiba Ali Hassan, Eline Wydooghe, Osvaldo Américo Bogado Pascottini, Anders Johannisson, Jane M. Morrell, Wojciech Nizanski, and Ann Van Soom. 2022. “Influence of Single Layer Centrifugation with Canicoll on Semen Freezability in Dogs.” ANIMALS 12 (6). doi:10.3390/ani12060714.
Vancouver
1.
Domain G, Ali Hassan H, Wydooghe E, Bogado Pascottini OA, Johannisson A, Morrell JM, et al. Influence of single layer centrifugation with canicoll on semen freezability in dogs. ANIMALS. 2022;12(6).
IEEE
[1]
G. Domain et al., “Influence of single layer centrifugation with canicoll on semen freezability in dogs,” ANIMALS, vol. 12, no. 6, 2022.
@article{8749722,
  abstract     = {{Simple Summary Freezing dog semen is not always possible due to low quality sperm or poor survival during freezing. In order to make this assisted reproductive technique available to a larger number of dogs, this study investigated the benefit of selecting the best spermatozoa before freezing using single layer centrifugation (SLC). The results indicated that this technique was effective in separating spermatozoa according to their quality, although this resulted in losing some good quality spermatozoa. After thawing, spermatozoa centrifuged by SLC were of better quality than after standard centrifugation. However, spermatozoa from suboptimal quality semen did not survive freezing as well as spermatozoa from semen of optimal quality, even after SLC. Single layer centrifugation, therefore, makes it possible to obtain better quality spermatozoa after thawing but is not sufficient on its own to improve the inferior freezing ability of spermatozoa from suboptimal quality semen. So far, eighteen pups were born after insemination with SLC-selected frozen-thawed semen, proving that these selected spermatozoa remain fertile. This study evaluated how semen selection by single layer centrifugation (SLC) with Canicoll affects semen freezability in dogs. A total of eighteen ejaculates, collected from dogs with optimal and suboptimal semen quality (optimal: normal morphology (NM) >= 80%, n = 9; suboptimal: NM between 60 and 79%, n = 9), were divided into two aliquots and subjected to standard centrifugation or SLC before cryopreservation. Motility, NM, membrane integrity, mitochondrial membrane potential (MMP), and DNA integrity were improved in fresh samples after SLC, regardless of semen quality, but at the expense of some good quality spermatozoa. After thawing, NM and membrane integrity were improved in SLC-selected semen in both semen qualities. Interestingly, MMP was also higher but only in optimal quality semen. Still, spermatozoa from suboptimal quality semen did not survive freezing to the same extent as spermatozoa from optimal quality semen, even after selecting superior spermatozoa. Semen selection with Canicoll is, therefore, an effective technique to isolate a subpopulation of high-quality spermatozoa and obtain sperm samples of better quality after thawing, but is not sufficient to improve the intrinsic inferior freezability of suboptimal quality semen. So far, eighteen pups were born after insemination with SLC-selected frozen-thawed semen, proving that these selected spermatozoa remain fertile.}},
  articleno    = {{714}},
  author       = {{Domain, Guillaume and Ali Hassan, Hiba and Wydooghe, Eline and Bogado Pascottini, Osvaldo Américo and Johannisson, Anders and Morrell, Jane M. and Nizanski, Wojciech and Van Soom, Ann}},
  issn         = {{2076-2615}},
  journal      = {{ANIMALS}},
  keywords     = {{SPERM DNA FRAGMENTATION,COLLOID CENTRIFUGATION,LIPID-PEROXIDATION,GENETIC DIVERSITY,OXIDATIVE STRESS,STALLION SEMEN,FROZEN,CRYOPRESERVATION,QUALITY,INSEMINATION,canine,fertility,semen selection,cryopreservation,semen quality}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{13}},
  title        = {{Influence of single layer centrifugation with canicoll on semen freezability in dogs}},
  url          = {{http://dx.doi.org/10.3390/ani12060714}},
  volume       = {{12}},
  year         = {{2022}},
}

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