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Quercetin mitigates endothelial activation in a novel intestinal-endothelial-monocyte/macrophage coculture setup

Hanne Vissenaekens (UGent) , Charlotte Grootaert (UGent) , Katleen Raes (UGent) , Julie De Munck (UGent) , Guy Smagghe (UGent) , Nico Boon (UGent) and John Van Camp (UGent)
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Abstract
Atherosclerosis initiation is associated with a pro-inflammatory state of the endothelium. Quercetin is a flavonoid abundantly present in plant-based foods, with a possible impact on cardiovascular health. In this study, the effects of quercetin on lipopolysaccharide (LPS)-mediated endothelial inflammation and monocyte adhesion and migration, which are initial steps of the atherogenic process, are studied. Novel in vitro multicellular models simulating the intestinal-endothelial-monocytes/macrophages axis allowed to combine relevant intestinal flavonoid absorption, metabolism and efflux, and the consequent bioactivity towards peripheral endothelial cells. In this triple coculture, quercetin exposure decreased monocyte adhesion to and macrophage migration through an LPS-stressed endothelium, and this was associated with significantly lower levels of soluble vascular cell adhesion molecule-1 (sVCAM-1). Furthermore, quercetin decreased the pro-inflammatory cell environment upon LPS-induced endothelial activation, in terms of tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), interleukin-8 (IL-8), and sVCAM-1 expression. These findings highlight a mode-of-action by which quercetin may positively impact the initial states of atherosclerosis under more physiologically relevant conditions in terms of quercetin concentrations, metabolites, and intercellular crosstalk.
Keywords
coculture cell model, quercetin, 1 popolysaccharide, endothelial activation, monocyte adhesion, inflammatory markers, ADHESION MOLECULE EXPRESSION, IN-VITRO, CARDIOVASCULAR-DISEASE, DIETARY POLYPHENOLS, FLAVONOID INTAKE, INFLAMMATION, DYSFUNCTION, HEALTH, DIFFERENTIATION, GLUCURONIDES

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MLA
Vissenaekens, Hanne, et al. “Quercetin Mitigates Endothelial Activation in a Novel Intestinal-Endothelial-Monocyte/Macrophage Coculture Setup.” INFLAMMATION, 2022, doi:10.1007/s10753-022-01645-w.
APA
Vissenaekens, H., Grootaert, C., Raes, K., De Munck, J., Smagghe, G., Boon, N., & Van Camp, J. (2022). Quercetin mitigates endothelial activation in a novel intestinal-endothelial-monocyte/macrophage coculture setup. INFLAMMATION. https://doi.org/10.1007/s10753-022-01645-w
Chicago author-date
Vissenaekens, Hanne, Charlotte Grootaert, Katleen Raes, Julie De Munck, Guy Smagghe, Nico Boon, and John Van Camp. 2022. “Quercetin Mitigates Endothelial Activation in a Novel Intestinal-Endothelial-Monocyte/Macrophage Coculture Setup.” INFLAMMATION. https://doi.org/10.1007/s10753-022-01645-w.
Chicago author-date (all authors)
Vissenaekens, Hanne, Charlotte Grootaert, Katleen Raes, Julie De Munck, Guy Smagghe, Nico Boon, and John Van Camp. 2022. “Quercetin Mitigates Endothelial Activation in a Novel Intestinal-Endothelial-Monocyte/Macrophage Coculture Setup.” INFLAMMATION. doi:10.1007/s10753-022-01645-w.
Vancouver
1.
Vissenaekens H, Grootaert C, Raes K, De Munck J, Smagghe G, Boon N, et al. Quercetin mitigates endothelial activation in a novel intestinal-endothelial-monocyte/macrophage coculture setup. INFLAMMATION. 2022;
IEEE
[1]
H. Vissenaekens et al., “Quercetin mitigates endothelial activation in a novel intestinal-endothelial-monocyte/macrophage coculture setup,” INFLAMMATION, 2022.
@article{8749697,
  abstract     = {{Atherosclerosis initiation is associated with a pro-inflammatory state of the endothelium. Quercetin is a flavonoid abundantly present in plant-based foods, with a possible impact on cardiovascular health. In this study, the effects of quercetin on lipopolysaccharide (LPS)-mediated endothelial inflammation and monocyte adhesion and migration, which are initial steps of the atherogenic process, are studied. Novel in vitro multicellular models simulating the intestinal-endothelial-monocytes/macrophages axis allowed to combine relevant intestinal flavonoid absorption, metabolism and efflux, and the consequent bioactivity towards peripheral endothelial cells. In this triple coculture, quercetin exposure decreased monocyte adhesion to and macrophage migration through an LPS-stressed endothelium, and this was associated with significantly lower levels of soluble vascular cell adhesion molecule-1 (sVCAM-1). Furthermore, quercetin decreased the pro-inflammatory cell environment upon LPS-induced endothelial activation, in terms of tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), interleukin-8 (IL-8), and sVCAM-1 expression. These findings highlight a mode-of-action by which quercetin may positively impact the initial states of atherosclerosis under more physiologically relevant conditions in terms of quercetin concentrations, metabolites, and intercellular crosstalk.}},
  author       = {{Vissenaekens, Hanne and Grootaert, Charlotte and Raes, Katleen and De Munck, Julie and Smagghe, Guy and Boon, Nico and Van Camp, John}},
  issn         = {{0360-3997}},
  journal      = {{INFLAMMATION}},
  keywords     = {{coculture cell model,quercetin,1 popolysaccharide,endothelial activation,monocyte adhesion,inflammatory markers,ADHESION MOLECULE EXPRESSION,IN-VITRO,CARDIOVASCULAR-DISEASE,DIETARY POLYPHENOLS,FLAVONOID INTAKE,INFLAMMATION,DYSFUNCTION,HEALTH,DIFFERENTIATION,GLUCURONIDES}},
  language     = {{eng}},
  title        = {{Quercetin mitigates endothelial activation in a novel intestinal-endothelial-monocyte/macrophage coculture setup}},
  url          = {{http://dx.doi.org/10.1007/s10753-022-01645-w}},
  year         = {{2022}},
}

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