Advanced search
1 file | 2.11 MB Add to list

Late embryogenesis abundant (LEA)5 regulates translation in mitochondria and chloroplasts to enhance growth and stress tolerance

Author
Organization
Abstract
The late embryogenesis abundant (LEA) protein family are small, highly hydrophilic proteins that are involved in stress tolerance. The small (10 kD) Arabidopsis LEA5 protein is predominantly expressed in leaves in the dark, the levels of LEA5 transcripts decreasing rapidly upon illumination. However, LEA5 is strongly expressed in leaves in the light when plants are exposed to stress. Localization assays suggest that LEA5 is dually localized to the chloroplasts and mitochondria. Tandem affinity purification analysis revealed LEA5 interacts with chloroplast proteins, particularly the DEAD-box ATP-dependent RNA helicase 22 (RH22), which is essential for RNA processing in plastids. Split YFP analysis confirmed the interaction between RH22 and LEA5 in chloroplasts. While changes in the abundance of LEA5 in mutants and transgenic plants had no effect on RNA processing in chloroplasts, the abundance of translated protein products were decreased in transgenic plants constitutively expressing LEA5 (OEX 2-5) and increased in lea5 knockout mutants. The abundance of translated mitochondrial protein products and mitochondrial electron transport rates were increased in OEX 2-5 plants and decreased in lea5 mutants. Barley lines transformed with Arabidopsis LEA5showed increased biomass and seed yields relative to controls. Moreover, the drought-induced inhibition of photosynthesis was greater in all of the LEA5-expressing barley lines than the wildtype We conclude that LEA5 is required for the regulation of translation, particularly in plants exposed to stress, acting to switch autotrophic to heterotrophic metabolism.
Keywords
Respiration, Mitochondria, Chloroplasts, translation, signalling

Downloads

  • Karpinska et al. 2022 Frontiers Plant Science 13 875799.pdf
    • full text (Published version)
    • |
    • open access
    • |
    • PDF
    • |
    • 2.11 MB

Citation

Please use this url to cite or link to this publication:

MLA
Karpinska, Barbara, et al. “Late Embryogenesis Abundant (LEA)5 Regulates Translation in Mitochondria and Chloroplasts to Enhance Growth and Stress Tolerance.” FRONTIERS IN PLANT SCIENCE, vol. 13, 2022, doi:10.3389/fpls.2022.875799.
APA
Karpinska, B., Nurhayatirazak, N., Shaw, D., Plumb, W., Van De Slijke, E., Stephens, J., … Foyer, C. (2022). Late embryogenesis abundant (LEA)5 regulates translation in mitochondria and chloroplasts to enhance growth and stress tolerance. FRONTIERS IN PLANT SCIENCE, 13. https://doi.org/10.3389/fpls.2022.875799
Chicago author-date
Karpinska, Barbara, Nurhayati Nurhayatirazak, Daniela Shaw, William Plumb, Eveline Van De Slijke, Jennifer Stephens, Geert De Jaeger, Monika Murcha, and Christine Foyer. 2022. “Late Embryogenesis Abundant (LEA)5 Regulates Translation in Mitochondria and Chloroplasts to Enhance Growth and Stress Tolerance.” FRONTIERS IN PLANT SCIENCE 13. https://doi.org/10.3389/fpls.2022.875799.
Chicago author-date (all authors)
Karpinska, Barbara, Nurhayati Nurhayatirazak, Daniela Shaw, William Plumb, Eveline Van De Slijke, Jennifer Stephens, Geert De Jaeger, Monika Murcha, and Christine Foyer. 2022. “Late Embryogenesis Abundant (LEA)5 Regulates Translation in Mitochondria and Chloroplasts to Enhance Growth and Stress Tolerance.” FRONTIERS IN PLANT SCIENCE 13. doi:10.3389/fpls.2022.875799.
Vancouver
1.
Karpinska B, Nurhayatirazak N, Shaw D, Plumb W, Van De Slijke E, Stephens J, et al. Late embryogenesis abundant (LEA)5 regulates translation in mitochondria and chloroplasts to enhance growth and stress tolerance. FRONTIERS IN PLANT SCIENCE. 2022;13.
IEEE
[1]
B. Karpinska et al., “Late embryogenesis abundant (LEA)5 regulates translation in mitochondria and chloroplasts to enhance growth and stress tolerance,” FRONTIERS IN PLANT SCIENCE, vol. 13, 2022.
@article{8748868,
  abstract     = {{The late embryogenesis abundant (LEA) protein family are small, highly hydrophilic proteins that are involved in stress tolerance. The small (10 kD) Arabidopsis LEA5 protein is predominantly expressed in leaves in the dark, the levels of LEA5 transcripts decreasing rapidly upon illumination. However, LEA5 is strongly expressed in leaves in the light when plants are exposed to stress. Localization assays suggest that LEA5 is dually localized to the chloroplasts and mitochondria. Tandem affinity purification analysis revealed LEA5 interacts with chloroplast proteins, particularly the DEAD-box ATP-dependent RNA helicase 22 (RH22), which is essential for RNA processing in plastids. Split YFP analysis confirmed the interaction between RH22 and LEA5 in chloroplasts. While changes in the abundance of LEA5 in mutants and transgenic plants had no effect on RNA processing in chloroplasts, the abundance of translated protein products were decreased in transgenic plants constitutively expressing LEA5 (OEX 2-5) and increased in lea5 knockout mutants. The abundance of translated mitochondrial protein products and mitochondrial electron transport rates were increased in OEX 2-5 plants and decreased in lea5 mutants. Barley lines transformed with Arabidopsis LEA5showed increased biomass and seed yields relative to controls. Moreover, the drought-induced inhibition of photosynthesis was greater in all of the LEA5-expressing barley lines than the wildtype We conclude that LEA5 is required for the regulation of translation, particularly in plants exposed to stress, acting to switch autotrophic to heterotrophic metabolism.}},
  articleno    = {{875799}},
  author       = {{Karpinska, Barbara and Nurhayatirazak, Nurhayati and Shaw, Daniela and Plumb, William and Van De Slijke, Eveline and Stephens, Jennifer and De Jaeger, Geert and Murcha, Monika and Foyer, Christine}},
  issn         = {{1664-462X}},
  journal      = {{FRONTIERS IN PLANT SCIENCE}},
  keywords     = {{Respiration,Mitochondria,Chloroplasts,translation,signalling}},
  language     = {{eng}},
  pages        = {{13}},
  title        = {{Late embryogenesis abundant (LEA)5 regulates translation in mitochondria and chloroplasts to enhance growth and stress tolerance}},
  url          = {{http://dx.doi.org/10.3389/fpls.2022.875799}},
  volume       = {{13}},
  year         = {{2022}},
}

Altmetric
View in Altmetric