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Paraburkholderia gardini sp. nov. and Paraburkholderia saeva sp. nov. : novel aromatic compound degrading bacteria isolated from garden and forest soil samples

Sarah Vanwijnsberghe (UGent) , Charlotte Peeters (UGent) , Margo Cnockaert (UGent) , Evelien De Canck (UGent) and Peter Vandamme (UGent)
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Abstract
Three forest and four botanical garden top soil isolates with unique MALDI-TOF mass spectra were identified as Paraburkholderia strains closely related to Paraburkholderia sartisoli through recA gene sequence analysis. OrthoANIu, digital DNA-DNA hybridization analyses and phylogenomic analyses demonstrated that the five strains represented two new Paraburkholderia species closely related to P. sartisoli. The genome of strain LMG 31841(T) had a cumulative size of 6.3 Mb and a G + C content of 62.64 mol%; strain LMG 32171(T) had a genome size of 5.8 Mb and a G + C content of 62.91 mol%. Hemolysis on horse blood agar, beta-galactosidase and phosphoamidase activity, and assimilation of adipic acid and trisodium citrate allowed phenotypic differentiation of strains LMG 31841(T), LMG 32171(T) and P. sartisoli LMG 24000(T). An analysis of the genomic potential for aromatic compound degradation yielded additional differences among strains representing these three species, but also highlighted some discrepancies between the presence of genes and pathways, and the phenotype revealed through growth experiments using a mineral salts medium supplemented with single aromatic compounds as carbon sources. We propose to classify all isolates from the present study into two novel Paraburkholderia species, for which we propose the names Paraburkholderia gardini with LMG 32171(T)(=CECT 30344(T)) as the type strain, and Paraburkholderia saeva with LMG 31841(T) (=CECT 30338(T)) as the type strain. (C)& nbsp;2022 Elsevier GmbH. All rights reserved.
Keywords
Applied Microbiology and Biotechnology, Ecology, Evolution, Behavior and Systematics, Microbiology, Paraburkholderia, Novel species, Microdiversity, Aromatic compound degradation, Comparative genomics, BURKHOLDERIA, QUALITY, GENES

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MLA
Vanwijnsberghe, Sarah, et al. “Paraburkholderia Gardini Sp. Nov. and Paraburkholderia Saeva Sp. Nov. : Novel Aromatic Compound Degrading Bacteria Isolated from Garden and Forest Soil Samples.” SYSTEMATIC AND APPLIED MICROBIOLOGY, vol. 45, no. 3, 2022, doi:10.1016/j.syapm.2022.126318.
APA
Vanwijnsberghe, S., Peeters, C., Cnockaert, M., De Canck, E., & Vandamme, P. (2022). Paraburkholderia gardini sp. nov. and Paraburkholderia saeva sp. nov. : novel aromatic compound degrading bacteria isolated from garden and forest soil samples. SYSTEMATIC AND APPLIED MICROBIOLOGY, 45(3). https://doi.org/10.1016/j.syapm.2022.126318
Chicago author-date
Vanwijnsberghe, Sarah, Charlotte Peeters, Margo Cnockaert, Evelien De Canck, and Peter Vandamme. 2022. “Paraburkholderia Gardini Sp. Nov. and Paraburkholderia Saeva Sp. Nov. : Novel Aromatic Compound Degrading Bacteria Isolated from Garden and Forest Soil Samples.” SYSTEMATIC AND APPLIED MICROBIOLOGY 45 (3). https://doi.org/10.1016/j.syapm.2022.126318.
Chicago author-date (all authors)
Vanwijnsberghe, Sarah, Charlotte Peeters, Margo Cnockaert, Evelien De Canck, and Peter Vandamme. 2022. “Paraburkholderia Gardini Sp. Nov. and Paraburkholderia Saeva Sp. Nov. : Novel Aromatic Compound Degrading Bacteria Isolated from Garden and Forest Soil Samples.” SYSTEMATIC AND APPLIED MICROBIOLOGY 45 (3). doi:10.1016/j.syapm.2022.126318.
Vancouver
1.
Vanwijnsberghe S, Peeters C, Cnockaert M, De Canck E, Vandamme P. Paraburkholderia gardini sp. nov. and Paraburkholderia saeva sp. nov. : novel aromatic compound degrading bacteria isolated from garden and forest soil samples. SYSTEMATIC AND APPLIED MICROBIOLOGY. 2022;45(3).
IEEE
[1]
S. Vanwijnsberghe, C. Peeters, M. Cnockaert, E. De Canck, and P. Vandamme, “Paraburkholderia gardini sp. nov. and Paraburkholderia saeva sp. nov. : novel aromatic compound degrading bacteria isolated from garden and forest soil samples,” SYSTEMATIC AND APPLIED MICROBIOLOGY, vol. 45, no. 3, 2022.
@article{8748508,
  abstract     = {{Three forest and four botanical garden top soil isolates with unique MALDI-TOF mass spectra were identified as Paraburkholderia strains closely related to Paraburkholderia sartisoli through recA gene sequence analysis. OrthoANIu, digital DNA-DNA hybridization analyses and phylogenomic analyses demonstrated that the five strains represented two new Paraburkholderia species closely related to P. sartisoli. The genome of strain LMG 31841(T) had a cumulative size of 6.3 Mb and a G + C content of 62.64 mol%; strain LMG 32171(T) had a genome size of 5.8 Mb and a G + C content of 62.91 mol%. Hemolysis on horse blood agar, beta-galactosidase and phosphoamidase activity, and assimilation of adipic acid and trisodium citrate allowed phenotypic differentiation of strains LMG 31841(T), LMG 32171(T) and P. sartisoli LMG 24000(T). An analysis of the genomic potential for aromatic compound degradation yielded additional differences among strains representing these three species, but also highlighted some discrepancies between the presence of genes and pathways, and the phenotype revealed through growth experiments using a mineral salts medium supplemented with single aromatic compounds as carbon sources. We propose to classify all isolates from the present study into two novel Paraburkholderia species, for which we propose the names Paraburkholderia gardini with LMG 32171(T)(=CECT 30344(T)) as the type strain, and Paraburkholderia saeva with LMG 31841(T) (=CECT 30338(T)) as the type strain. (C)& nbsp;2022 Elsevier GmbH. All rights reserved.}},
  articleno    = {{126318}},
  author       = {{Vanwijnsberghe, Sarah and Peeters, Charlotte and Cnockaert, Margo and De Canck, Evelien and Vandamme, Peter}},
  issn         = {{0723-2020}},
  journal      = {{SYSTEMATIC AND APPLIED MICROBIOLOGY}},
  keywords     = {{Applied Microbiology and Biotechnology,Ecology,Evolution,Behavior and Systematics,Microbiology,Paraburkholderia,Novel species,Microdiversity,Aromatic compound degradation,Comparative genomics,BURKHOLDERIA,QUALITY,GENES}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{16}},
  title        = {{Paraburkholderia gardini sp. nov. and Paraburkholderia saeva sp. nov. : novel aromatic compound degrading bacteria isolated from garden and forest soil samples}},
  url          = {{http://dx.doi.org/10.1016/j.syapm.2022.126318}},
  volume       = {{45}},
  year         = {{2022}},
}

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