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Tree species diversity and forest edge density jointly shape the gut microbiota composition in juvenile great tits (Parus major)

Evy Goossens (UGent) , Roschong Boonyarittichaikij (UGent) , Daan Dekeukeleire (UGent) , Lionel Hertzog (UGent) , Sarah Van Praet (UGent) , Frank Pasmans (UGent) , Dries Bonte (UGent) , Kris Verheyen (UGent) , Luc Lens (UGent) , An Martel (UGent) , et al.
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Abstract
Despite the microbiome’s key role in health and fitness, little is known about the environmental factors shaping the gut microbiome of wild birds. With habitat fragmentation being recognised as a major threat to biological diversity, we here determined how forest structure influences the bacterial species richness and diversity of wild great tit nestlings (Parus major). Using an Illumina metabarcoding approach which amplifies the 16S bacterial ribosomal RNA gene, we measured gut microbiota diversity and composition from 49 great tit nestlings, originating from 23 different nests that were located in 22 different study plots across a gradient of forest fragmentation and tree species diversity. Per nest, an average microbiome was determined on which the influence of tree species (composition and richness) and forest fragmentation (fragment area and edge density) was examined and whether this was linked to host characteristics (body condition and fledging success). We found an interaction effect of edge density with tree species richness or composition on both the microbial richness (alpha diversity: Chao1 and Shannon) and community structure (beta diversity: weighted and unweighted UniFrac). No significant short-term impact was observed of the overall faecal microbiome on host characteristics, but rather an adverse effect of specific bacterial genera on fledging success. These results highlight the influence of environmental factors on the microbial richness as well as the phylogenetic diversity during a life stage where the birds’ microbiota is shaped, which could lead to long-term consequences for host fitness.
Keywords
Microbiology (medical), Microbiology, great tits (Parus major), faeces, microbiota, tree species diversity, forest fragmentation, HABITAT FRAGMENTATION, POSTFLEDGING SURVIVAL, BLUE, PATHOGENS, ABUNDANCE, BACTERIA, BIRDS, RNA

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Citation

Please use this url to cite or link to this publication:

MLA
Goossens, Evy, et al. “Tree Species Diversity and Forest Edge Density Jointly Shape the Gut Microbiota Composition in Juvenile Great Tits (Parus Major).” FRONTIERS IN MICROBIOLOGY, vol. 13, 2022, doi:10.3389/fmicb.2022.790189.
APA
Goossens, E., Boonyarittichaikij, R., Dekeukeleire, D., Hertzog, L., Van Praet, S., Pasmans, F., … Verbrugghe, E. (2022). Tree species diversity and forest edge density jointly shape the gut microbiota composition in juvenile great tits (Parus major). FRONTIERS IN MICROBIOLOGY, 13. https://doi.org/10.3389/fmicb.2022.790189
Chicago author-date
Goossens, Evy, Roschong Boonyarittichaikij, Daan Dekeukeleire, Lionel Hertzog, Sarah Van Praet, Frank Pasmans, Dries Bonte, et al. 2022. “Tree Species Diversity and Forest Edge Density Jointly Shape the Gut Microbiota Composition in Juvenile Great Tits (Parus Major).” FRONTIERS IN MICROBIOLOGY 13. https://doi.org/10.3389/fmicb.2022.790189.
Chicago author-date (all authors)
Goossens, Evy, Roschong Boonyarittichaikij, Daan Dekeukeleire, Lionel Hertzog, Sarah Van Praet, Frank Pasmans, Dries Bonte, Kris Verheyen, Luc Lens, An Martel, and Elin Verbrugghe. 2022. “Tree Species Diversity and Forest Edge Density Jointly Shape the Gut Microbiota Composition in Juvenile Great Tits (Parus Major).” FRONTIERS IN MICROBIOLOGY 13. doi:10.3389/fmicb.2022.790189.
Vancouver
1.
Goossens E, Boonyarittichaikij R, Dekeukeleire D, Hertzog L, Van Praet S, Pasmans F, et al. Tree species diversity and forest edge density jointly shape the gut microbiota composition in juvenile great tits (Parus major). FRONTIERS IN MICROBIOLOGY. 2022;13.
IEEE
[1]
E. Goossens et al., “Tree species diversity and forest edge density jointly shape the gut microbiota composition in juvenile great tits (Parus major),” FRONTIERS IN MICROBIOLOGY, vol. 13, 2022.
@article{8745719,
  abstract     = {{Despite the microbiome’s key role in health and fitness, little is known about the environmental factors shaping the gut microbiome of wild birds. With habitat fragmentation being recognised as a major threat to biological diversity, we here determined how forest structure influences the bacterial species richness and diversity of wild great tit nestlings (Parus major). Using an Illumina metabarcoding approach which amplifies the 16S bacterial ribosomal RNA gene, we measured gut microbiota diversity and composition from 49 great tit nestlings, originating from 23 different nests that were located in 22 different study plots across a gradient of forest fragmentation and tree species diversity. Per nest, an average microbiome was determined on which the influence of tree species (composition and richness) and forest fragmentation (fragment area and edge density) was examined and whether this was linked to host characteristics (body condition and fledging success). We found an interaction effect of edge density with tree species richness or composition on both the microbial richness (alpha diversity: Chao1 and Shannon) and community structure (beta diversity: weighted and unweighted UniFrac). No significant short-term impact was observed of the overall faecal microbiome on host characteristics, but rather an adverse effect of specific bacterial genera on fledging success. These results highlight the influence of environmental factors on the microbial richness as well as the phylogenetic diversity during a life stage where the birds’ microbiota is shaped, which could lead to long-term consequences for host fitness.}},
  articleno    = {{790189}},
  author       = {{Goossens, Evy and Boonyarittichaikij, Roschong and Dekeukeleire, Daan and Hertzog, Lionel and Van Praet, Sarah and Pasmans, Frank and Bonte, Dries and Verheyen, Kris and Lens, Luc and Martel, An and Verbrugghe, Elin}},
  issn         = {{1664-302X}},
  journal      = {{FRONTIERS IN MICROBIOLOGY}},
  keywords     = {{Microbiology (medical),Microbiology,great tits (Parus major),faeces,microbiota,tree species diversity,forest fragmentation,HABITAT FRAGMENTATION,POSTFLEDGING SURVIVAL,BLUE,PATHOGENS,ABUNDANCE,BACTERIA,BIRDS,RNA}},
  language     = {{eng}},
  pages        = {{12}},
  title        = {{Tree species diversity and forest edge density jointly shape the gut microbiota composition in juvenile great tits (Parus major)}},
  url          = {{http://dx.doi.org/10.3389/fmicb.2022.790189}},
  volume       = {{13}},
  year         = {{2022}},
}

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