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Selective Campylobacter detection and quantification in poultry : a sensor tool for detecting the cause of a common zoonosis at its source

Stella Givanoudi, Peter Cornelis, Geertrui Rasschaert, Gideon Wackers, Heiko Iken, David Rolka, Derick Yongabi, Johan Robbens, Michael J. Schoning, Marc Heyndrickx (UGent) , et al.
(2021) SENSORS AND ACTUATORS B-CHEMICAL. 332.
Author
Organization
Abstract
Thermotolerant Campylobacter bacteria, most notably Campylobacter jejuni and Campylobacter coli, are a major cause of human foodborne gastroenteritis, which is usually related to consumption of contaminated poultry. In this work, we present a sensor and the associated assay for the on-site detection of the prevalent species C. jejuni and C. coli. The sensor uses surface-imprinted polymer (SIP) layers as selective, biomimetic recognition elements in combination with a modified heat-transfer method (M-HTM) as a label-free, quantitative readout principle. The selectivity for C. coli and C. jejuni was evaluated against six other morphologically similar Campylobacterales species, confirming that the sensor is selective at species level while responding uniformly to different strains within the same species. For the relevant matrix, that is chicken cecal droppings suspended in PBS buffer, the detection limits are 1.1 x 10(3) CFU/mL for C. coli and 2.7 x 10(4) CFU/mL for C. jejuni, which is both low enough for a meaningful diagnostic test. The sensor concept requires only a minimum of sample preparation and a given concentration can be measured within less than one hour: Both are important assets for on-site detection such as on a poultry farm or in a slaughterhouse, keeping in mind that Campylobacter detection with established methods in analytical laboratories takes 2-4 days for obtaining the result.
Keywords
HEAT-TRANSFER-METHOD, ESCHERICHIA-COLI, HELICOBACTER-PULLORUM, SYNTHETIC, RECEPTORS, IMPRINTED POLYMERS, THERMAL TRANSPORT, CELL DETECTION, LABEL-FREE, JEJUNI, IDENTIFICATION, Campylobacter species, Poultry farming, Zoonosis, Biomimetic sensors, Surface-imprinted polymers, Heat-transfer method

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Citation

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MLA
Givanoudi, Stella, et al. “Selective Campylobacter Detection and Quantification in Poultry : A Sensor Tool for Detecting the Cause of a Common Zoonosis at Its Source.” SENSORS AND ACTUATORS B-CHEMICAL, vol. 332, 2021, doi:10.1016/j.snb.2021.129484.
APA
Givanoudi, S., Cornelis, P., Rasschaert, G., Wackers, G., Iken, H., Rolka, D., … Wagner, P. (2021). Selective Campylobacter detection and quantification in poultry : a sensor tool for detecting the cause of a common zoonosis at its source. SENSORS AND ACTUATORS B-CHEMICAL, 332. https://doi.org/10.1016/j.snb.2021.129484
Chicago author-date
Givanoudi, Stella, Peter Cornelis, Geertrui Rasschaert, Gideon Wackers, Heiko Iken, David Rolka, Derick Yongabi, et al. 2021. “Selective Campylobacter Detection and Quantification in Poultry : A Sensor Tool for Detecting the Cause of a Common Zoonosis at Its Source.” SENSORS AND ACTUATORS B-CHEMICAL 332. https://doi.org/10.1016/j.snb.2021.129484.
Chicago author-date (all authors)
Givanoudi, Stella, Peter Cornelis, Geertrui Rasschaert, Gideon Wackers, Heiko Iken, David Rolka, Derick Yongabi, Johan Robbens, Michael J. Schoning, Marc Heyndrickx, and Patrick Wagner. 2021. “Selective Campylobacter Detection and Quantification in Poultry : A Sensor Tool for Detecting the Cause of a Common Zoonosis at Its Source.” SENSORS AND ACTUATORS B-CHEMICAL 332. doi:10.1016/j.snb.2021.129484.
Vancouver
1.
Givanoudi S, Cornelis P, Rasschaert G, Wackers G, Iken H, Rolka D, et al. Selective Campylobacter detection and quantification in poultry : a sensor tool for detecting the cause of a common zoonosis at its source. SENSORS AND ACTUATORS B-CHEMICAL. 2021;332.
IEEE
[1]
S. Givanoudi et al., “Selective Campylobacter detection and quantification in poultry : a sensor tool for detecting the cause of a common zoonosis at its source,” SENSORS AND ACTUATORS B-CHEMICAL, vol. 332, 2021.
@article{8743951,
  abstract     = {{Thermotolerant Campylobacter bacteria, most notably Campylobacter jejuni and Campylobacter coli, are a major cause of human foodborne gastroenteritis, which is usually related to consumption of contaminated poultry. In this work, we present a sensor and the associated assay for the on-site detection of the prevalent species C. jejuni and C. coli. The sensor uses surface-imprinted polymer (SIP) layers as selective, biomimetic recognition elements in combination with a modified heat-transfer method (M-HTM) as a label-free, quantitative readout principle. The selectivity for C. coli and C. jejuni was evaluated against six other morphologically similar Campylobacterales species, confirming that the sensor is selective at species level while responding uniformly to different strains within the same species. For the relevant matrix, that is chicken cecal droppings suspended in PBS buffer, the detection limits are 1.1 x 10(3) CFU/mL for C. coli and 2.7 x 10(4) CFU/mL for C. jejuni, which is both low enough for a meaningful diagnostic test. The sensor concept requires only a minimum of sample preparation and a given concentration can be measured within less than one hour: Both are important assets for on-site detection such as on a poultry farm or in a slaughterhouse, keeping in mind that Campylobacter detection with established methods in analytical laboratories takes 2-4 days for obtaining the result.}},
  articleno    = {{129484}},
  author       = {{Givanoudi, Stella and Cornelis, Peter and Rasschaert, Geertrui and Wackers, Gideon and Iken, Heiko and Rolka, David and Yongabi, Derick and Robbens, Johan and Schoning, Michael J. and Heyndrickx, Marc and Wagner, Patrick}},
  issn         = {{0925-4005}},
  journal      = {{SENSORS AND ACTUATORS B-CHEMICAL}},
  keywords     = {{HEAT-TRANSFER-METHOD,ESCHERICHIA-COLI,HELICOBACTER-PULLORUM,SYNTHETIC,RECEPTORS,IMPRINTED POLYMERS,THERMAL TRANSPORT,CELL DETECTION,LABEL-FREE,JEJUNI,IDENTIFICATION,Campylobacter species,Poultry farming,Zoonosis,Biomimetic sensors,Surface-imprinted polymers,Heat-transfer method}},
  language     = {{eng}},
  pages        = {{11}},
  title        = {{Selective Campylobacter detection and quantification in poultry : a sensor tool for detecting the cause of a common zoonosis at its source}},
  url          = {{http://doi.org/10.1016/j.snb.2021.129484}},
  volume       = {{332}},
  year         = {{2021}},
}
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