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Transient nuclear lamin A/C accretion aids in recovery from vapor nanobubble-induced permeabilisation of the plasma membrane

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Abstract
Vapor nanobubble (VNB) photoporation is a physical method for intracellular delivery that has gained significant interest in the past decade. It has successfully been used to introduce molecular cargo of diverse nature into different cell types with high throughput and minimal cytotoxicity. For translational purposes, it is important to understand whether and how photoporation affects cell homeostasis. To obtain a comprehensive view on the transcriptional rewiring that takes place after VNB photoporation, we performed a longitudinal shotgun RNA-sequencing experiment. Six hours after photoporation, we found a marked upregulation of LMNA transcripts as well as their protein products, the A-type lamins. At the same time point, we observed a significant increase in several heterochromatin marks, suggesting a global stiffening of the nucleus. These molecular features vanished 24 h after photoporation. Since VNB-induced chromatin condensation was prolonged in LMNA knockout cells, A-type lamins may be required for restoring the nucleus to its original state. Selective depletion of A-type lamins reduced cell viability after VNB photoporation, while pharmacological stimulation of LMNA transcription increased the percentage of successfully transfected cells that survived after photoporation. Therefore, our results suggest that cells respond to VNB photoporation by temporary upregulation of A-type lamins to facilitate their recovery.
Keywords
Cell Biology, Cellular and Molecular Neuroscience, Pharmacology, Molecular Biology, Molecular Medicine, Vapor nanobubbles, Gold nanoparticles, Photoporation, Plasma membrane, A-type lamins, Chromatin, CHROMATIN, DELIVERY, SONOPORATION, PHOTOPORATION, STRESS, CELLS, HETEROCHROMATIN, INDUCTION, EFFICIENT, APOPTOSIS

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MLA
Houthaeve, Gaëlle, et al. “Transient Nuclear Lamin A/C Accretion Aids in Recovery from Vapor Nanobubble-Induced Permeabilisation of the Plasma Membrane.” CELLULAR AND MOLECULAR LIFE SCIENCES, vol. 79, no. 1, 2022, doi:10.1007/s00018-021-04099-9.
APA
Houthaeve, G., García-Díaz Barriga, G., Stremersch, S., De Keersmaecker, H., Fraire, J., Vandesompele, J., … De Vos, W. (2022). Transient nuclear lamin A/C accretion aids in recovery from vapor nanobubble-induced permeabilisation of the plasma membrane. CELLULAR AND MOLECULAR LIFE SCIENCES, 79(1). https://doi.org/10.1007/s00018-021-04099-9
Chicago author-date
Houthaeve, Gaëlle, Gerardo García-Díaz Barriga, Stephan Stremersch, Herlinde De Keersmaecker, Juan Fraire, Jo Vandesompele, Pieter Mestdagh, Stefaan De Smedt, Kevin Braeckmans, and Winnok De Vos. 2022. “Transient Nuclear Lamin A/C Accretion Aids in Recovery from Vapor Nanobubble-Induced Permeabilisation of the Plasma Membrane.” CELLULAR AND MOLECULAR LIFE SCIENCES 79 (1). https://doi.org/10.1007/s00018-021-04099-9.
Chicago author-date (all authors)
Houthaeve, Gaëlle, Gerardo García-Díaz Barriga, Stephan Stremersch, Herlinde De Keersmaecker, Juan Fraire, Jo Vandesompele, Pieter Mestdagh, Stefaan De Smedt, Kevin Braeckmans, and Winnok De Vos. 2022. “Transient Nuclear Lamin A/C Accretion Aids in Recovery from Vapor Nanobubble-Induced Permeabilisation of the Plasma Membrane.” CELLULAR AND MOLECULAR LIFE SCIENCES 79 (1). doi:10.1007/s00018-021-04099-9.
Vancouver
1.
Houthaeve G, García-Díaz Barriga G, Stremersch S, De Keersmaecker H, Fraire J, Vandesompele J, et al. Transient nuclear lamin A/C accretion aids in recovery from vapor nanobubble-induced permeabilisation of the plasma membrane. CELLULAR AND MOLECULAR LIFE SCIENCES. 2022;79(1).
IEEE
[1]
G. Houthaeve et al., “Transient nuclear lamin A/C accretion aids in recovery from vapor nanobubble-induced permeabilisation of the plasma membrane,” CELLULAR AND MOLECULAR LIFE SCIENCES, vol. 79, no. 1, 2022.
@article{8743612,
  abstract     = {{Vapor nanobubble (VNB) photoporation is a physical method for intracellular delivery that has gained significant interest in the past decade. It has successfully been used to introduce molecular cargo of diverse nature into different cell types with high throughput and minimal cytotoxicity. For translational purposes, it is important to understand whether and how photoporation affects cell homeostasis. To obtain a comprehensive view on the transcriptional rewiring that takes place after VNB photoporation, we performed a longitudinal shotgun RNA-sequencing experiment. Six hours after photoporation, we found a marked upregulation of LMNA transcripts as well as their protein products, the A-type lamins. At the same time point, we observed a significant increase in several heterochromatin marks, suggesting a global stiffening of the nucleus. These molecular features vanished 24 h after photoporation. Since VNB-induced chromatin condensation was prolonged in LMNA knockout cells, A-type lamins may be required for restoring the nucleus to its original state. Selective depletion of A-type lamins reduced cell viability after VNB photoporation, while pharmacological stimulation of LMNA transcription increased the percentage of successfully transfected cells that survived after photoporation. Therefore, our results suggest that cells respond to VNB photoporation by temporary upregulation of A-type lamins to facilitate their recovery.}},
  articleno    = {{23}},
  author       = {{Houthaeve, Gaëlle and García-Díaz Barriga, Gerardo and Stremersch, Stephan and De Keersmaecker, Herlinde and Fraire, Juan and Vandesompele, Jo and Mestdagh, Pieter and De Smedt, Stefaan and Braeckmans, Kevin and De Vos, Winnok}},
  issn         = {{1420-682X}},
  journal      = {{CELLULAR AND MOLECULAR LIFE SCIENCES}},
  keywords     = {{Cell Biology,Cellular and Molecular Neuroscience,Pharmacology,Molecular Biology,Molecular Medicine,Vapor nanobubbles,Gold nanoparticles,Photoporation,Plasma membrane,A-type lamins,Chromatin,CHROMATIN,DELIVERY,SONOPORATION,PHOTOPORATION,STRESS,CELLS,HETEROCHROMATIN,INDUCTION,EFFICIENT,APOPTOSIS}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{13}},
  title        = {{Transient nuclear lamin A/C accretion aids in recovery from vapor nanobubble-induced permeabilisation of the plasma membrane}},
  url          = {{http://doi.org/10.1007/s00018-021-04099-9}},
  volume       = {{79}},
  year         = {{2022}},
}

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