@article{8743022,
  abstract     = {{Prostate cancer metastasis has an enormous impact on the mortality of cancer patients. Factors involved in cancer progression and metastasis are known to be key players in microgravity (mu g)-driven three-dimensional (3D) cancer spheroid formation. We investigated PC-3 prostate cancer cells for 30 min, 2, 4 and 24 h on the random positioning machine (RPM), a device simulating mu g on Earth. After a 24 h RPM-exposure, the cells could be divided into two groups: one grew as 3D multicellular spheroids (MCS), the other one as adherent monolayer (AD). No signs of apoptosis were visible. Among others, we focused on cytokines involved in the events of metastasis and MCS formation. After 24 h of exposure, in the MCS group we measured an increase in ACTB, MSN, COL1A1, LAMA3, FN1, TIMP1, FLT1, EGFR1, IL1A, IL6, CXCL8, and HIF1A mRNA expression, and in the AD group an elevation of LAMA3, COL1A1, FN1, MMP9, VEGFA, IL6, and CXCL8 mRNAs compared to samples subjected to 1 g conditions. Significant downregulations in AD cells were detected in the mRNA levels of TUBB, KRT8, IL1B, IL7, PIK3CB, AKT1 and MTOR after 24 h. The release of collagen-1 alpha 1 and fibronectin protein in the supernatant was decreased, whereas the secretion of IL-6 was elevated in 24 h RPM samples. The secretion of IL-1 alpha, IL-1 beta, IL-7, IL-2, IL-8, IL-17, TNF-alpha, laminin, MMP-2, TIMP-1, osteopontin and EGF was not significantly altered after 24 h compared to 1 g conditions. The release of soluble factors was significantly reduced after 2 h (IL-1 alpha, IL-2, IL-7, IL-8, IL-17, TNF-alpha, collagen-1 alpha 1, MMP-2, osteopontin) and elevated after 4 h (IL-1 beta, IL-2, IL-6, IL-7, IL-8, TNF-alpha, laminin) in RPM samples. Taken together, simulated mu g induced 3D growth of PC-3 cancer cells combined with a differential expression of the cytokines IL-1 alpha, IL-1 beta, IL-6 and IL-8, supporting their involvement in growth and progression of prostate cancer cells.}},
  articleno    = {{841017}},
  author       = {{Dietrichs, Dorothea and Grimm, Daniela and Sahana, Jayashree and Melnik, Daniela and Corydon, Thomas J. and Wehland, Markus and Krüger, Marcus and Vermeesen, Randy and Baselet, Bjorn and Baatout, Sarah and Hybel, Trine Engelbrecht and Kahlert, Stefan and Schulz, Herbert and Infanger, Manfred and Kopp, Sascha}},
  issn         = {{2296-634X}},
  journal      = {{FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY}},
  keywords     = {{prostate cancer,cytokines,interleukins,cytoskeleton,extracellular matrix,PAM signaling,prostate cancer cells and microgravity,INDUCIBLE FACTOR 1-ALPHA,SPHEROID FORMATION,GENE-EXPRESSION,EXTRACELLULAR-MATRIX,INCREASES APOPTOSIS,INTERLEUKIN-6,CYTOSKELETON,PROTEINS,ALTERS,LINES}},
  language     = {{eng}},
  pages        = {{21}},
  title        = {{Three-dimensional growth of prostate cancer cells exposed to simulated microgravity}},
  url          = {{http://doi.org/10.3389/fcell.2022.841017}},
  volume       = {{10}},
  year         = {{2022}},
}

Altmetric

View in Altmetric

Web of Science

Times cited: