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Plasmodesmata play pivotal role in sucrose supply to Meloidogyne graminicola-caused giant cells in rice

(2021) MOLECULAR PLANT PATHOLOGY. 22(5). p.539-550
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Abstract
On infection, plant-parasitic nematodes establish feeding sites in roots from which they take up carbohydrates among other nutrients. Knowledge on how carbohydrates are supplied to the nematodes' feeding sites is limited. Here, gene expression analyses showed that RNA levels of OsSWEET11 to OsSWEET15 were extremely low in both Meloidogyne graminicola (Mg)-caused galls and noninoculated roots. All the rice sucrose transporter genes, OsSUT1 to OsSUT5, were either down-regulated in Mg-caused galls compared with noninoculated rice roots or had very low transcript abundance. OsSUT1 was the only gene up-regulated in galls, at 14 days postinoculation (dpi), after being highly down-regulated at 3 and 7 dpi. OsSUT4 was down-regulated at 3 dpi. No noticeable OsSUTs promoter activities were detected in Mg-caused galls of pOsSUT1 to -5::GUS rice lines. Loading experiments with carboxyfluorescein diacetate (CFDA) demonstrated that symplastic connections exist between phloem and Mg-caused giant cells (GCs). According to data from OsGNS5- and OsGSL2-overexpressing rice plants that had decreased and increased callose deposition, respectively, callose negatively affected Mg parasitism and sucrose supply to Mg-caused GCs. Our results suggest that plasmodesmata-mediated sucrose transport plays a pivotal role in sucrose supply from rice root phloem to Mg-caused GCs, and OsSWEET11 to -15 and OsSUTs are not major players in it, although further functional analysis is needed for OsSUT1 and OsSUT4.
Keywords
callose deposition, giant cells, Meloidogyne graminicola, OsSUT, s, plasmodesmata, sucrose transport

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MLA
Xu, Li-he, et al. “Plasmodesmata Play Pivotal Role in Sucrose Supply to Meloidogyne Graminicola-Caused Giant Cells in Rice.” MOLECULAR PLANT PATHOLOGY, vol. 22, no. 5, 2021, pp. 539–50, doi:10.1111/mpp.13042.
APA
Xu, L., Xiao, L., Xiao, Y., Peng, D., Xiao, X., Huang, W., … Wang, G. (2021). Plasmodesmata play pivotal role in sucrose supply to Meloidogyne graminicola-caused giant cells in rice. MOLECULAR PLANT PATHOLOGY, 22(5), 539–550. https://doi.org/10.1111/mpp.13042
Chicago author-date
Xu, Li-he, Li-ying Xiao, Yan-nong Xiao, De-liang Peng, Xue-qiong Xiao, Wen-kun Huang, Godelieve Gheysen, and Gao-feng Wang. 2021. “Plasmodesmata Play Pivotal Role in Sucrose Supply to Meloidogyne Graminicola-Caused Giant Cells in Rice.” MOLECULAR PLANT PATHOLOGY 22 (5): 539–50. https://doi.org/10.1111/mpp.13042.
Chicago author-date (all authors)
Xu, Li-he, Li-ying Xiao, Yan-nong Xiao, De-liang Peng, Xue-qiong Xiao, Wen-kun Huang, Godelieve Gheysen, and Gao-feng Wang. 2021. “Plasmodesmata Play Pivotal Role in Sucrose Supply to Meloidogyne Graminicola-Caused Giant Cells in Rice.” MOLECULAR PLANT PATHOLOGY 22 (5): 539–550. doi:10.1111/mpp.13042.
Vancouver
1.
Xu L, Xiao L, Xiao Y, Peng D, Xiao X, Huang W, et al. Plasmodesmata play pivotal role in sucrose supply to Meloidogyne graminicola-caused giant cells in rice. MOLECULAR PLANT PATHOLOGY. 2021;22(5):539–50.
IEEE
[1]
L. Xu et al., “Plasmodesmata play pivotal role in sucrose supply to Meloidogyne graminicola-caused giant cells in rice,” MOLECULAR PLANT PATHOLOGY, vol. 22, no. 5, pp. 539–550, 2021.
@article{8743001,
  abstract     = {{On infection, plant-parasitic nematodes establish feeding sites in roots from which they take up carbohydrates among other nutrients. Knowledge on how carbohydrates are supplied to the nematodes' feeding sites is limited. Here, gene expression analyses showed that RNA levels of OsSWEET11 to OsSWEET15 were extremely low in both Meloidogyne graminicola (Mg)-caused galls and noninoculated roots. All the rice sucrose transporter genes, OsSUT1 to OsSUT5, were either down-regulated in Mg-caused galls compared with noninoculated rice roots or had very low transcript abundance. OsSUT1 was the only gene up-regulated in galls, at 14 days postinoculation (dpi), after being highly down-regulated at 3 and 7 dpi. OsSUT4 was down-regulated at 3 dpi. No noticeable OsSUTs promoter activities were detected in Mg-caused galls of pOsSUT1 to -5::GUS rice lines. Loading experiments with carboxyfluorescein diacetate (CFDA) demonstrated that symplastic connections exist between phloem and Mg-caused giant cells (GCs). According to data from OsGNS5- and OsGSL2-overexpressing rice plants that had decreased and increased callose deposition, respectively, callose negatively affected Mg parasitism and sucrose supply to Mg-caused GCs. Our results suggest that plasmodesmata-mediated sucrose transport plays a pivotal role in sucrose supply from rice root phloem to Mg-caused GCs, and OsSWEET11 to -15 and OsSUTs are not major players in it, although further functional analysis is needed for OsSUT1 and OsSUT4.}},
  author       = {{Xu, Li-he and Xiao, Li-ying and Xiao, Yan-nong and Peng, De-liang and Xiao, Xue-qiong and Huang, Wen-kun and Gheysen, Godelieve and Wang, Gao-feng}},
  issn         = {{1464-6722}},
  journal      = {{MOLECULAR PLANT PATHOLOGY}},
  keywords     = {{callose deposition,giant cells,Meloidogyne graminicola,OsSUT,s,plasmodesmata,sucrose transport}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{539--550}},
  title        = {{Plasmodesmata play pivotal role in sucrose supply to Meloidogyne graminicola-caused giant cells in rice}},
  url          = {{http://doi.org/10.1111/mpp.13042}},
  volume       = {{22}},
  year         = {{2021}},
}

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