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A fit-for-purpose LC-MS/MS method for the analysis of selected Streptococcal quorum sensing peptides in human saliva

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Abstract
The development of analytical methods for the detection of peptides at the nanomolar level can be challenging. Peptides can suffer from adsorption, rendering the detection of peptides at these low levels difficult. A subset of peptides are the quorum sensing peptides, which are bacterial communication molecules demonstrating possible host effects as well. However, their direct presence in human biofluids has only rarely been reported. Therefore, a UHPLC-MS/MS method capable of detecting 15 selected Streptococcal competence stimulating quorum sensing peptides at the nanomolar level in human saliva was developed. This method, using an anti-adsorption diluent, was applied on saliva samples obtained from 38 healthy donors. Six donors did have a positive hit for at least one of three competence stimulating quorum sensing peptides using a triple quadrupole assay. These observations indicate that Streptococcus species produce quorum sensing peptides in the human oral cavity.
Keywords
Clinical Biochemistry, Spectroscopy, Drug Discovery, Pharmaceutical Science, Analytical Chemistry, Quorum sensing peptides, Streptococcus, Peptide adsorption, LC-MS, Saliva, COMPETENCE, PNEUMONIAE, PHEROTYPE

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Citation

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MLA
Verbeke, Frederick, et al. “A Fit-for-Purpose LC-MS/MS Method for the Analysis of Selected Streptococcal Quorum Sensing Peptides in Human Saliva.” JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, vol. 213, 2022, doi:10.1016/j.jpba.2022.114594.
APA
Verbeke, F., Van der borght, K., De Spiegeleer, A., Debunne, N., Janssens, Y., Wynendaele, E., & De Spiegeleer, B. (2022). A fit-for-purpose LC-MS/MS method for the analysis of selected Streptococcal quorum sensing peptides in human saliva. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 213. https://doi.org/10.1016/j.jpba.2022.114594
Chicago author-date
Verbeke, Frederick, Kevin Van der borght, Anton De Spiegeleer, Nathan Debunne, Yorick Janssens, Evelien Wynendaele, and Bart De Spiegeleer. 2022. “A Fit-for-Purpose LC-MS/MS Method for the Analysis of Selected Streptococcal Quorum Sensing Peptides in Human Saliva.” JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 213. https://doi.org/10.1016/j.jpba.2022.114594.
Chicago author-date (all authors)
Verbeke, Frederick, Kevin Van der borght, Anton De Spiegeleer, Nathan Debunne, Yorick Janssens, Evelien Wynendaele, and Bart De Spiegeleer. 2022. “A Fit-for-Purpose LC-MS/MS Method for the Analysis of Selected Streptococcal Quorum Sensing Peptides in Human Saliva.” JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 213. doi:10.1016/j.jpba.2022.114594.
Vancouver
1.
Verbeke F, Van der borght K, De Spiegeleer A, Debunne N, Janssens Y, Wynendaele E, et al. A fit-for-purpose LC-MS/MS method for the analysis of selected Streptococcal quorum sensing peptides in human saliva. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. 2022;213.
IEEE
[1]
F. Verbeke et al., “A fit-for-purpose LC-MS/MS method for the analysis of selected Streptococcal quorum sensing peptides in human saliva,” JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, vol. 213, 2022.
@article{8742840,
  abstract     = {{The development of analytical methods for the detection of peptides at the nanomolar level can be challenging. Peptides can suffer from adsorption, rendering the detection of peptides at these low levels difficult. A subset of peptides are the quorum sensing peptides, which are bacterial communication molecules demonstrating possible host effects as well. However, their direct presence in human biofluids has only rarely been reported. Therefore, a UHPLC-MS/MS method capable of detecting 15 selected Streptococcal competence stimulating quorum sensing peptides at the nanomolar level in human saliva was developed. This method, using an anti-adsorption diluent, was applied on saliva samples obtained from 38 healthy donors. Six donors did have a positive hit for at least one of three competence stimulating quorum sensing peptides using a triple quadrupole assay. These observations indicate that Streptococcus species produce quorum sensing peptides in the human oral cavity.}},
  articleno    = {{114594}},
  author       = {{Verbeke, Frederick and Van der borght, Kevin and De Spiegeleer, Anton and Debunne, Nathan and Janssens, Yorick and Wynendaele, Evelien and De Spiegeleer, Bart}},
  issn         = {{0731-7085}},
  journal      = {{JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS}},
  keywords     = {{Clinical Biochemistry,Spectroscopy,Drug Discovery,Pharmaceutical Science,Analytical Chemistry,Quorum sensing peptides,Streptococcus,Peptide adsorption,LC-MS,Saliva,COMPETENCE,PNEUMONIAE,PHEROTYPE}},
  language     = {{eng}},
  pages        = {{9}},
  title        = {{A fit-for-purpose LC-MS/MS method for the analysis of selected Streptococcal quorum sensing peptides in human saliva}},
  url          = {{http://dx.doi.org/10.1016/j.jpba.2022.114594}},
  volume       = {{213}},
  year         = {{2022}},
}

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