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Subacute ruminal acidosis phenotypes in periparturient dairy cows differ in ruminal and salivary bacteria and in the in vitro fermentative activity of their ruminal microbiota

(2022) JOURNAL OF DAIRY SCIENCE. 105(5). p.3969-3987
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Abstract
Both ruminal microbial structure and functionality might play a role in inter-individual variation in susceptibility for subacute rumen acidosis (SARA) observed in dairy cows. The aims of this study were to determine whether differences between cows with distinct SARA susceptibility were reflected in distinct (1) ruminal microbial communities, (2) salivary bacterial communities, and (3) fermentative capacity of ruminal microbiota assessed in vitro. To test this hypothesis, rumen samples were collected via an esophageal tube on 21 d postpartum from 38 multiparous Holstein cows, which were classified into 4 groups differing in median and mean time of reticular pH below 6 as well as area under the curve of pH below 6.0. During the 21 d postpartum, all cows within a group fulfilled following criteria: susceptible (S, n = 10; mean or median >= 180 min/d), moderately susceptible (MS, n = 7; 60 min/d < mean time of pH below 6 < 180 min/d, and median time of pH below 6 <180 min/d), moderately unsusceptible (MU, n = 11; 10 min/d < mean < 60 min/d, and median time of pH below 6 <= 30 min/d), or unsusceptible (U, n = 10; median = 0 min/d, and mean <10 min/d). Groups did not differ in total daily dry matter intake nor in total, roughage, or concentrate intake during daily 6-h time intervals. Rumen bacterial alpha-diversity did not differ among groups, but beta-diversity varied and bacterial 16S rRNA gene copy numbers were lower in S compared with U cows. The relative abundance of genera Streptococcus, Sharpea, Prevotellaceae_YAB2003, Succinivibrionaceae_UCG-001, Ruminococcus, and Ruminococcaceae_UCG-001 were higher in S compared with U cows. In contrast, Lachnospiraceae_ND3007 and Oscillospiraceae_V9D2013 were more abundant in U cows. Although pH-associated, inter-animal differences were also observed in the salivary bacteria, common differences in ruminal and salivary bacterial genera were limited. The functionality of the rumen microbiota was evaluated in vitro through exposure of the microbial inoculum of S and U cows to an anaerobic buffer at pH 5.8 and 6.8, in the presence of sterile supernatant of their own and of dry cows' rumen fluid (2 x 2 design). Generally, the S inoculum produced more volatile fatty acids, except at low pH with dry cows' supernatant, where volatile fatty acid production was completely impaired and lactate accumulation was highest. Compared with the microbes of U cows, microbes of S cows showed less fermentative activity in situations with 2 stress factors (low pH and an unfamiliar environment, i.e., rumen fluid supernatant of dry cows).
Keywords
subacute ruminal acidosis, periparturient period, inter-animal variation, bacterial community, in vitro fermentative activity

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MLA
Yang, Hong, et al. “Subacute Ruminal Acidosis Phenotypes in Periparturient Dairy Cows Differ in Ruminal and Salivary Bacteria and in the in Vitro Fermentative Activity of Their Ruminal Microbiota.” JOURNAL OF DAIRY SCIENCE, vol. 105, no. 5, 2022, pp. 3969–87, doi:10.3168/jds.2021-21115.
APA
Yang, H., Heirbaut, S., Jeyanathan, J., Jing, X., De Neve, N., Vandaele, L., & Fievez, V. (2022). Subacute ruminal acidosis phenotypes in periparturient dairy cows differ in ruminal and salivary bacteria and in the in vitro fermentative activity of their ruminal microbiota. JOURNAL OF DAIRY SCIENCE, 105(5), 3969–3987. https://doi.org/10.3168/jds.2021-21115
Chicago author-date
Yang, Hong, Stijn Heirbaut, Jeyamalar Jeyanathan, Xiaoping Jing, Nympha De Neve, L. Vandaele, and Veerle Fievez. 2022. “Subacute Ruminal Acidosis Phenotypes in Periparturient Dairy Cows Differ in Ruminal and Salivary Bacteria and in the in Vitro Fermentative Activity of Their Ruminal Microbiota.” JOURNAL OF DAIRY SCIENCE 105 (5): 3969–87. https://doi.org/10.3168/jds.2021-21115.
Chicago author-date (all authors)
Yang, Hong, Stijn Heirbaut, Jeyamalar Jeyanathan, Xiaoping Jing, Nympha De Neve, L. Vandaele, and Veerle Fievez. 2022. “Subacute Ruminal Acidosis Phenotypes in Periparturient Dairy Cows Differ in Ruminal and Salivary Bacteria and in the in Vitro Fermentative Activity of Their Ruminal Microbiota.” JOURNAL OF DAIRY SCIENCE 105 (5): 3969–3987. doi:10.3168/jds.2021-21115.
Vancouver
1.
Yang H, Heirbaut S, Jeyanathan J, Jing X, De Neve N, Vandaele L, et al. Subacute ruminal acidosis phenotypes in periparturient dairy cows differ in ruminal and salivary bacteria and in the in vitro fermentative activity of their ruminal microbiota. JOURNAL OF DAIRY SCIENCE. 2022;105(5):3969–87.
IEEE
[1]
H. Yang et al., “Subacute ruminal acidosis phenotypes in periparturient dairy cows differ in ruminal and salivary bacteria and in the in vitro fermentative activity of their ruminal microbiota,” JOURNAL OF DAIRY SCIENCE, vol. 105, no. 5, pp. 3969–3987, 2022.
@article{8742779,
  abstract     = {{Both ruminal microbial structure and functionality might play a role in inter-individual variation in susceptibility for subacute rumen acidosis (SARA) observed in dairy cows. The aims of this study were to determine whether differences between cows with distinct SARA susceptibility were reflected in distinct (1) ruminal microbial communities, (2) salivary bacterial communities, and (3) fermentative capacity of ruminal microbiota assessed in vitro. To test this hypothesis, rumen samples were collected via an esophageal tube on 21 d postpartum from 38 multiparous Holstein cows, which were classified into 4 groups differing in median and mean time of reticular pH below 6 as well as area under the curve of pH below 6.0. During the 21 d postpartum, all cows within a group fulfilled following criteria: susceptible (S, n = 10; mean or median >= 180 min/d), moderately susceptible (MS, n = 7; 60 min/d < mean time of pH below 6 < 180 min/d, and median time of pH below 6 <180 min/d), moderately unsusceptible (MU, n = 11; 10 min/d < mean < 60 min/d, and median time of pH below 6 <= 30 min/d), or unsusceptible (U, n = 10; median = 0 min/d, and mean <10 min/d). Groups did not differ in total daily dry matter intake nor in total, roughage, or concentrate intake during daily 6-h time intervals. Rumen bacterial alpha-diversity did not differ among groups, but beta-diversity varied and bacterial 16S rRNA gene copy numbers were lower in S compared with U cows. The relative abundance of genera Streptococcus, Sharpea, Prevotellaceae_YAB2003, Succinivibrionaceae_UCG-001, Ruminococcus, and Ruminococcaceae_UCG-001 were higher in S compared with U cows. In contrast, Lachnospiraceae_ND3007 and Oscillospiraceae_V9D2013 were more abundant in U cows. Although pH-associated, inter-animal differences were also observed in the salivary bacteria, common differences in ruminal and salivary bacterial genera were limited. The functionality of the rumen microbiota was evaluated in vitro through exposure of the microbial inoculum of S and U cows to an anaerobic buffer at pH 5.8 and 6.8, in the presence of sterile supernatant of their own and of dry cows' rumen fluid (2 x 2 design). Generally, the S inoculum produced more volatile fatty acids, except at low pH with dry cows' supernatant, where volatile fatty acid production was completely impaired and lactate accumulation was highest. Compared with the microbes of U cows, microbes of S cows showed less fermentative activity in situations with 2 stress factors (low pH and an unfamiliar environment, i.e., rumen fluid supernatant of dry cows).}},
  author       = {{Yang, Hong and Heirbaut, Stijn and Jeyanathan, Jeyamalar and Jing, Xiaoping and De Neve, Nympha and Vandaele, L. and Fievez, Veerle}},
  issn         = {{0022-0302}},
  journal      = {{JOURNAL OF DAIRY SCIENCE}},
  keywords     = {{subacute ruminal acidosis,periparturient period,inter-animal variation,bacterial community,in vitro fermentative activity}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{3969--3987}},
  title        = {{Subacute ruminal acidosis phenotypes in periparturient dairy cows differ in ruminal and salivary bacteria and in the in vitro fermentative activity of their ruminal microbiota}},
  url          = {{http://doi.org/10.3168/jds.2021-21115}},
  volume       = {{105}},
  year         = {{2022}},
}

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