Maximizing nutrient recycling from digestate for production of protein-rich microalgae for animal feed application
- Author
- Jai Sankar Seelam (UGent) , Marcella Fernandes De Souza (UGent) , Peter Chaerle (UGent) , Bernard Willems, Evi Michels (UGent) , Wim Vyverman (UGent) and Erik Meers (UGent)
- Organization
- Abstract
- The integration of phototrophic microalgal production and anaerobic digestion can recycle excess nutrients across European surplus hotspots to produce protein-rich biomass for nutritional applications. However, the challenging physico-chemical properties of raw digestate constrain microalgal growth and limit digestate valorization potential. This study focused on the pre-treatment of food waste-based digestate using paper-filtration to improve its properties for cultivating Desmodesmus sp. and Chlorella vulgaris. The microalgal growth performance in paper-filtered digestate (PFD, 10 mu m-pore size) was then compared to growth in membrane-filtered digestate (MFD, 0.2 mu m-pore size). A microplate-based screening coupled with Cytation device assessment of PFD and MFD samples after dilution and with/without phosphorus supplementation showed that PFD was the best substrate. Moreover, phosphorus supplementation resulted in improved growth at higher digestate concentrations (5-10% v/v PFD), indicating the importance of using a balanced growth medium to increase the volumetric usage of digestate. Results were validated in a 3-L bioreactor at 10% PFD with phosphorus supplementation, reaching a biomass concentration of 2.4 g L-1 with a protein and carbohydrate content of 67% and 13% w/w respectively. This trial indicates that paper-filtration is a promising pre-treatment technique to maximize digestate recycling and deliver a sustainable animal feed-grade protein alternative.
- Keywords
- ANAEROBIC DIGESTATE, WASTE-WATER, REMOVAL, FOOD, OPTIMIZATION, CULTIVATION, FILTRATION, Animal feed, Digestate, Microalgae, Microplate experiments, Paper, filtration, Photobioreactor
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8742319
- MLA
- Seelam, Jai Sankar, et al. “Maximizing Nutrient Recycling from Digestate for Production of Protein-Rich Microalgae for Animal Feed Application.” CHEMOSPHERE, vol. 290, 2022, doi:10.1016/j.chemosphere.2021.133180.
- APA
- Seelam, J. S., Fernandes De Souza, M., Chaerle, P., Willems, B., Michels, E., Vyverman, W., & Meers, E. (2022). Maximizing nutrient recycling from digestate for production of protein-rich microalgae for animal feed application. CHEMOSPHERE, 290. https://doi.org/10.1016/j.chemosphere.2021.133180
- Chicago author-date
- Seelam, Jai Sankar, Marcella Fernandes De Souza, Peter Chaerle, Bernard Willems, Evi Michels, Wim Vyverman, and Erik Meers. 2022. “Maximizing Nutrient Recycling from Digestate for Production of Protein-Rich Microalgae for Animal Feed Application.” CHEMOSPHERE 290. https://doi.org/10.1016/j.chemosphere.2021.133180.
- Chicago author-date (all authors)
- Seelam, Jai Sankar, Marcella Fernandes De Souza, Peter Chaerle, Bernard Willems, Evi Michels, Wim Vyverman, and Erik Meers. 2022. “Maximizing Nutrient Recycling from Digestate for Production of Protein-Rich Microalgae for Animal Feed Application.” CHEMOSPHERE 290. doi:10.1016/j.chemosphere.2021.133180.
- Vancouver
- 1.Seelam JS, Fernandes De Souza M, Chaerle P, Willems B, Michels E, Vyverman W, et al. Maximizing nutrient recycling from digestate for production of protein-rich microalgae for animal feed application. CHEMOSPHERE. 2022;290.
- IEEE
- [1]J. S. Seelam et al., “Maximizing nutrient recycling from digestate for production of protein-rich microalgae for animal feed application,” CHEMOSPHERE, vol. 290, 2022.
@article{8742319,
abstract = {{The integration of phototrophic microalgal production and anaerobic digestion can recycle excess nutrients across European surplus hotspots to produce protein-rich biomass for nutritional applications. However, the challenging physico-chemical properties of raw digestate constrain microalgal growth and limit digestate valorization potential. This study focused on the pre-treatment of food waste-based digestate using paper-filtration to improve its properties for cultivating Desmodesmus sp. and Chlorella vulgaris. The microalgal growth performance in paper-filtered digestate (PFD, 10 mu m-pore size) was then compared to growth in membrane-filtered digestate (MFD, 0.2 mu m-pore size). A microplate-based screening coupled with Cytation device assessment of PFD and MFD samples after dilution and with/without phosphorus supplementation showed that PFD was the best substrate. Moreover, phosphorus supplementation resulted in improved growth at higher digestate concentrations (5-10% v/v PFD), indicating the importance of using a balanced growth medium to increase the volumetric usage of digestate. Results were validated in a 3-L bioreactor at 10% PFD with phosphorus supplementation, reaching a biomass concentration of 2.4 g L-1 with a protein and carbohydrate content of 67% and 13% w/w respectively. This trial indicates that paper-filtration is a promising pre-treatment technique to maximize digestate recycling and deliver a sustainable animal feed-grade protein alternative.}},
articleno = {{133180}},
author = {{Seelam, Jai Sankar and Fernandes De Souza, Marcella and Chaerle, Peter and Willems, Bernard and Michels, Evi and Vyverman, Wim and Meers, Erik}},
issn = {{0045-6535}},
journal = {{CHEMOSPHERE}},
keywords = {{ANAEROBIC DIGESTATE,WASTE-WATER,REMOVAL,FOOD,OPTIMIZATION,CULTIVATION,FILTRATION,Animal feed,Digestate,Microalgae,Microplate experiments,Paper,filtration,Photobioreactor}},
language = {{eng}},
pages = {{9}},
title = {{Maximizing nutrient recycling from digestate for production of protein-rich microalgae for animal feed application}},
url = {{http://dx.doi.org/10.1016/j.chemosphere.2021.133180}},
volume = {{290}},
year = {{2022}},
}
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