NanI sialidase contributes to toxin expression and host cell binding of Clostridium perfringens type G strain CP56 in vitro
- Author
- Lore Van Damme, Chana Callens (UGent) , Michelle Dargatz, Monika Flügel, Sarah Hark, Frank Thiemann, Stefan Pelzer, Richard Ducatelle (UGent) , Filip Van Immerseel (UGent) and Evy Goossens (UGent)
- Organization
- Project
- Abstract
- Necrotic enteritis, caused by NetB producing Clostridium perfringens type G strains, is a globally important poultry disease. An initial step in the pathogenesis of necrotic enteritis is the colonization and degradation of the intestinal mucus layer, a process in which C. perfringens sialidases - such as NanI sialidase - may play an important role. Sialidases cleave terminal sialic acid from complex carbohydrates on glycoconjugates, such as mucins. This study shows that NE-associated C. perfringens strain CP56 is able to use sialic acid (Neu5Ac) as a carbon source for bacterial growth. It is shown that supplementation of Neu5Ac in the growth medium does not only induce the production of extracellular sialidases of strain CP56, but also increases the production of both alpha toxin and NetB toxin. Moreover, it was found that pre-treating avian hepatocellular carcinoma cells (LMH cells) with the recombinant NanI sialidase increases the adherence of C. perfringens type G strain CP56 to these cells. As such, the data suggest an important role for sialidases in the pathogenesis of the disease.
- Keywords
- General Veterinary, General Medicine, Microbiology, Clostridium perfringens, Sialidase, Type G, NanI, NECROTIC ENTERITIS, PATHOGENESIS, CHICKENS
Downloads
-
(...).pdf
- full text (Published version)
- |
- UGent only
- |
- |
- 1.01 MB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8741098
- MLA
- Van Damme, Lore, et al. “NanI Sialidase Contributes to Toxin Expression and Host Cell Binding of Clostridium Perfringens Type G Strain CP56 in Vitro.” VETERINARY MICROBIOLOGY, vol. 266, 2022, doi:10.1016/j.vetmic.2022.109371.
- APA
- Van Damme, L., Callens, C., Dargatz, M., Flügel, M., Hark, S., Thiemann, F., … Goossens, E. (2022). NanI sialidase contributes to toxin expression and host cell binding of Clostridium perfringens type G strain CP56 in vitro. VETERINARY MICROBIOLOGY, 266. https://doi.org/10.1016/j.vetmic.2022.109371
- Chicago author-date
- Van Damme, Lore, Chana Callens, Michelle Dargatz, Monika Flügel, Sarah Hark, Frank Thiemann, Stefan Pelzer, Richard Ducatelle, Filip Van Immerseel, and Evy Goossens. 2022. “NanI Sialidase Contributes to Toxin Expression and Host Cell Binding of Clostridium Perfringens Type G Strain CP56 in Vitro.” VETERINARY MICROBIOLOGY 266. https://doi.org/10.1016/j.vetmic.2022.109371.
- Chicago author-date (all authors)
- Van Damme, Lore, Chana Callens, Michelle Dargatz, Monika Flügel, Sarah Hark, Frank Thiemann, Stefan Pelzer, Richard Ducatelle, Filip Van Immerseel, and Evy Goossens. 2022. “NanI Sialidase Contributes to Toxin Expression and Host Cell Binding of Clostridium Perfringens Type G Strain CP56 in Vitro.” VETERINARY MICROBIOLOGY 266. doi:10.1016/j.vetmic.2022.109371.
- Vancouver
- 1.Van Damme L, Callens C, Dargatz M, Flügel M, Hark S, Thiemann F, et al. NanI sialidase contributes to toxin expression and host cell binding of Clostridium perfringens type G strain CP56 in vitro. VETERINARY MICROBIOLOGY. 2022;266.
- IEEE
- [1]L. Van Damme et al., “NanI sialidase contributes to toxin expression and host cell binding of Clostridium perfringens type G strain CP56 in vitro,” VETERINARY MICROBIOLOGY, vol. 266, 2022.
@article{8741098,
abstract = {{Necrotic enteritis, caused by NetB producing Clostridium perfringens type G strains, is a globally important poultry disease. An initial step in the pathogenesis of necrotic enteritis is the colonization and degradation of the intestinal mucus layer, a process in which C. perfringens sialidases - such as NanI sialidase - may play an important role. Sialidases cleave terminal sialic acid from complex carbohydrates on glycoconjugates, such as mucins. This study shows that NE-associated C. perfringens strain CP56 is able to use sialic acid (Neu5Ac) as a carbon source for bacterial growth. It is shown that supplementation of Neu5Ac in the growth medium does not only induce the production of extracellular sialidases of strain CP56, but also increases the production of both alpha toxin and NetB toxin. Moreover, it was found that pre-treating avian hepatocellular carcinoma cells (LMH cells) with the recombinant NanI sialidase increases the adherence of C. perfringens type G strain CP56 to these cells. As such, the data suggest an important role for sialidases in the pathogenesis of the disease.}},
articleno = {{109371}},
author = {{Van Damme, Lore and Callens, Chana and Dargatz, Michelle and Flügel, Monika and Hark, Sarah and Thiemann, Frank and Pelzer, Stefan and Ducatelle, Richard and Van Immerseel, Filip and Goossens, Evy}},
issn = {{0378-1135}},
journal = {{VETERINARY MICROBIOLOGY}},
keywords = {{General Veterinary,General Medicine,Microbiology,Clostridium perfringens,Sialidase,Type G,NanI,NECROTIC ENTERITIS,PATHOGENESIS,CHICKENS}},
language = {{eng}},
pages = {{5}},
title = {{NanI sialidase contributes to toxin expression and host cell binding of Clostridium perfringens type G strain CP56 in vitro}},
url = {{http://doi.org/10.1016/j.vetmic.2022.109371}},
volume = {{266}},
year = {{2022}},
}
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: