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DNA methylation regulates transcription factor-specific neurodevelopmental but not sexually dimorphic gene expression dynamics in zebra finch telencephalon

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Abstract
Song learning in zebra finches (Taeniopygia guttata) is a prototypical example of a complex learned behavior, yet knowledge of the underlying molecular processes is limited. Therefore, we characterized transcriptomic (RNA-sequencing) and epigenomic (RRBS, reduced representation bisulfite sequencing; immunofluorescence) dynamics in matched zebra finch telencephalon samples of both sexes from 1 day post hatching (1 dph) to adulthood, spanning the critical period for song learning (20 and 65 dph). We identified extensive transcriptional neurodevelopmental changes during postnatal telencephalon development. DNA methylation was very low, yet increased over time, particularly in song control nuclei. Only a small fraction of the massive differential expression in the developing zebra finch telencephalon could be explained by differential CpG and CpH DNA methylation. However, a strong association between DNA methylation and age-dependent gene expression was found for various transcription factors (i.e., OTX2, AR, and FOS) involved in neurodevelopment. Incomplete dosage compensation, independent of DNA methylation, was found to be largely responsible for sexually dimorphic gene expression, with dosage compensation increasing throughout life. In conclusion, our results indicate that DNA methylation regulates neurodevelopmental gene expression dynamics through steering transcription factor activity, but does not explain sexually dimorphic gene expression patterns in zebra finch telencephalon.
Keywords
DNA methylation, zebra finch, brain development, epigenetics, song learning, dosage compensation, gene expression

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MLA
Diddens, Jolien, et al. “DNA Methylation Regulates Transcription Factor-Specific Neurodevelopmental but Not Sexually Dimorphic Gene Expression Dynamics in Zebra Finch Telencephalon.” FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, vol. 9, 2021, doi:10.3389/fcell.2021.583555.
APA
Diddens, J., Coussement, L., Frankl-Vilches, C., Majumdar, G., Steyaert, S., Ter Haar, S. M., … Vanden Berghe, W. (2021). DNA methylation regulates transcription factor-specific neurodevelopmental but not sexually dimorphic gene expression dynamics in zebra finch telencephalon. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 9. https://doi.org/10.3389/fcell.2021.583555
Chicago author-date
Diddens, Jolien, Louis Coussement, Carolina Frankl-Vilches, Gaurav Majumdar, Sandra Steyaert, Sita M. Ter Haar, Jeroen Galle, et al. 2021. “DNA Methylation Regulates Transcription Factor-Specific Neurodevelopmental but Not Sexually Dimorphic Gene Expression Dynamics in Zebra Finch Telencephalon.” FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 9. https://doi.org/10.3389/fcell.2021.583555.
Chicago author-date (all authors)
Diddens, Jolien, Louis Coussement, Carolina Frankl-Vilches, Gaurav Majumdar, Sandra Steyaert, Sita M. Ter Haar, Jeroen Galle, Ellen De Meester, Sarah De Keulenaer, Wim Van Criekinge, Charlotte A. Cornil, Jacques Balthazart, Annemie Van der Linden, Tim De Meyer, and Wim Vanden Berghe. 2021. “DNA Methylation Regulates Transcription Factor-Specific Neurodevelopmental but Not Sexually Dimorphic Gene Expression Dynamics in Zebra Finch Telencephalon.” FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 9. doi:10.3389/fcell.2021.583555.
Vancouver
1.
Diddens J, Coussement L, Frankl-Vilches C, Majumdar G, Steyaert S, Ter Haar SM, et al. DNA methylation regulates transcription factor-specific neurodevelopmental but not sexually dimorphic gene expression dynamics in zebra finch telencephalon. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY. 2021;9.
IEEE
[1]
J. Diddens et al., “DNA methylation regulates transcription factor-specific neurodevelopmental but not sexually dimorphic gene expression dynamics in zebra finch telencephalon,” FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, vol. 9, 2021.
@article{8741084,
  abstract     = {{Song learning in zebra finches (Taeniopygia guttata) is a prototypical example of a complex learned behavior, yet knowledge of the underlying molecular processes is limited. Therefore, we characterized transcriptomic (RNA-sequencing) and epigenomic (RRBS, reduced representation bisulfite sequencing; immunofluorescence) dynamics in matched zebra finch telencephalon samples of both sexes from 1 day post hatching (1 dph) to adulthood, spanning the critical period for song learning (20 and 65 dph). We identified extensive transcriptional neurodevelopmental changes during postnatal telencephalon development. DNA methylation was very low, yet increased over time, particularly in song control nuclei. Only a small fraction of the massive differential expression in the developing zebra finch telencephalon could be explained by differential CpG and CpH DNA methylation. However, a strong association between DNA methylation and age-dependent gene expression was found for various transcription factors (i.e., OTX2, AR, and FOS) involved in neurodevelopment. Incomplete dosage compensation, independent of DNA methylation, was found to be largely responsible for sexually dimorphic gene expression, with dosage compensation increasing throughout life. In conclusion, our results indicate that DNA methylation regulates neurodevelopmental gene expression dynamics through steering transcription factor activity, but does not explain sexually dimorphic gene expression patterns in zebra finch telencephalon.}},
  articleno    = {{583555}},
  author       = {{Diddens, Jolien and Coussement, Louis and Frankl-Vilches, Carolina and Majumdar, Gaurav and Steyaert, Sandra and Ter Haar, Sita M. and Galle, Jeroen and De Meester, Ellen and De Keulenaer, Sarah and Van Criekinge, Wim and Cornil, Charlotte A. and Balthazart, Jacques and Van der Linden, Annemie and De Meyer, Tim and Vanden Berghe, Wim}},
  issn         = {{2296-634X}},
  journal      = {{FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY}},
  keywords     = {{DNA methylation,zebra finch,brain development,epigenetics,song learning,dosage compensation,gene expression}},
  language     = {{eng}},
  pages        = {{19}},
  title        = {{DNA methylation regulates transcription factor-specific neurodevelopmental but not sexually dimorphic gene expression dynamics in zebra finch telencephalon}},
  url          = {{http://dx.doi.org/10.3389/fcell.2021.583555}},
  volume       = {{9}},
  year         = {{2021}},
}

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