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Auxin analog-induced Ca 2+ signaling is not involved in inhibition of endosomal aggregation in Arabidopsis roots

Ren Wang (UGent) , Ellie Himschoot (UGent) , Matteo Grenzi, Jian Chen (UGent) , Alaeddine Safi (UGent) , Melanie Krebs, Karin Schumacher, Moritz Nowack (UGent) , Wolfgang Moeder, Keiko Yoshioka, et al.
(2022) JOURNAL OF EXPERIMENTAL BOTANY. 73(8). p.2308-2319
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Abstract
Much of what we know about the role of auxin in plant development derives from exogenous manipulations of auxin distribution and signaling, using inhibitors, auxins, and auxin analogs. In this context, synthetic auxin analogs, such as 1-naphthalene acetic acid (1-NAA), are often favored over the endogenous auxin, indole-3-acetic acid (IAA), in part due to their higher stability. While such auxin analogs have proven instrumental in revealing the various faces of auxin, they display in some cases bioactivities distinct from IAA. Here, we focused on the effect of auxin analogs on the accumulation of PIN proteins in brefeldin A-sensitive endosomal aggregations (BFA bodies), and correlation with the ability to elicit Ca2+ responses. For a set of commonly used auxin analogs, we evaluated if auxin analog-induced Ca2+ signaling inhibits PIN accumulation. Not all auxin analogs elicited a Ca2+ response, and their differential ability to elicit Ca2+ responses correlated partially with their ability to inhibit BFA-body formation. However, in tir1/afb and cngc14, 1-NAA-induced Ca2+ signaling was strongly impaired, yet 1-NAA still could inhibit PIN accumulation in BFA bodies. This demonstrates that TIR1/AFB-CNGC14-dependent Ca2+ signaling does not inhibit BFA body formation in Arabidopsis roots. Synthetic auxin analog-induced Ca(2+)signaling is independent of inhibition of PIN accumulation in brefeldin A-sensitive endosomal aggregations.
Keywords
Arabidopsis, Auxin-analogs, Calcium, PIN protein, endosomes, root, signaling, trafficking

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Citation

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MLA
Wang, Ren, et al. “Auxin Analog-Induced Ca 2+ Signaling Is Not Involved in Inhibition of Endosomal Aggregation in Arabidopsis Roots.” JOURNAL OF EXPERIMENTAL BOTANY, vol. 73, no. 8, 2022, pp. 2308–19, doi:10.1093/jxb/erac019.
APA
Wang, R., Himschoot, E., Grenzi, M., Chen, J., Safi, A., Krebs, M., … Vanneste, S. (2022). Auxin analog-induced Ca 2+ signaling is not involved in inhibition of endosomal aggregation in Arabidopsis roots. JOURNAL OF EXPERIMENTAL BOTANY, 73(8), 2308–2319. https://doi.org/10.1093/jxb/erac019
Chicago author-date
Wang, Ren, Ellie Himschoot, Matteo Grenzi, Jian Chen, Alaeddine Safi, Melanie Krebs, Karin Schumacher, et al. 2022. “Auxin Analog-Induced Ca 2+ Signaling Is Not Involved in Inhibition of Endosomal Aggregation in Arabidopsis Roots.” JOURNAL OF EXPERIMENTAL BOTANY 73 (8): 2308–19. https://doi.org/10.1093/jxb/erac019.
Chicago author-date (all authors)
Wang, Ren, Ellie Himschoot, Matteo Grenzi, Jian Chen, Alaeddine Safi, Melanie Krebs, Karin Schumacher, Moritz Nowack, Wolfgang Moeder, Keiko Yoshioka, Daniël Van Damme, Ive De Smet, Danny Geelen, Tom Beeckman, Jiří Friml, Alex Costa, and Steffen Vanneste. 2022. “Auxin Analog-Induced Ca 2+ Signaling Is Not Involved in Inhibition of Endosomal Aggregation in Arabidopsis Roots.” JOURNAL OF EXPERIMENTAL BOTANY 73 (8): 2308–2319. doi:10.1093/jxb/erac019.
Vancouver
1.
Wang R, Himschoot E, Grenzi M, Chen J, Safi A, Krebs M, et al. Auxin analog-induced Ca 2+ signaling is not involved in inhibition of endosomal aggregation in Arabidopsis roots. JOURNAL OF EXPERIMENTAL BOTANY. 2022;73(8):2308–19.
IEEE
[1]
R. Wang et al., “Auxin analog-induced Ca 2+ signaling is not involved in inhibition of endosomal aggregation in Arabidopsis roots,” JOURNAL OF EXPERIMENTAL BOTANY, vol. 73, no. 8, pp. 2308–2319, 2022.
@article{8738721,
  abstract     = {{Much of what we know about the role of auxin in plant development derives from exogenous manipulations of auxin distribution and signaling, using inhibitors, auxins, and auxin analogs. In this context, synthetic auxin analogs, such as 1-naphthalene acetic acid (1-NAA), are often favored over the endogenous auxin, indole-3-acetic acid (IAA), in part due to their higher stability. While such auxin analogs have proven instrumental in revealing the various faces of auxin, they display in some cases bioactivities distinct from IAA. Here, we focused on the effect of auxin analogs on the accumulation of PIN proteins in brefeldin A-sensitive endosomal aggregations (BFA bodies), and correlation with the ability to elicit Ca2+ responses. For a set of commonly used auxin analogs, we evaluated if auxin analog-induced Ca2+ signaling inhibits PIN accumulation. Not all auxin analogs elicited a Ca2+ response, and their differential ability to elicit Ca2+ responses correlated partially with their ability to inhibit BFA-body formation. However, in tir1/afb and cngc14, 1-NAA-induced Ca2+ signaling was strongly impaired, yet 1-NAA still could inhibit PIN accumulation in BFA bodies. This demonstrates that TIR1/AFB-CNGC14-dependent Ca2+ signaling does not inhibit BFA body formation in Arabidopsis roots.

Synthetic auxin analog-induced Ca(2+)signaling is independent of inhibition of PIN accumulation in brefeldin A-sensitive endosomal aggregations.}},
  author       = {{Wang, Ren and Himschoot, Ellie and Grenzi, Matteo and Chen, Jian and Safi, Alaeddine and Krebs, Melanie and Schumacher, Karin and Nowack, Moritz and Moeder, Wolfgang and Yoshioka, Keiko and Van Damme, Daniël and De Smet, Ive and Geelen, Danny and Beeckman, Tom and Friml, Jiří and Costa, Alex and Vanneste, Steffen}},
  issn         = {{0022-0957}},
  journal      = {{JOURNAL OF EXPERIMENTAL BOTANY}},
  keywords     = {{Arabidopsis,Auxin-analogs,Calcium,PIN protein,endosomes,root,signaling,trafficking}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{2308--2319}},
  title        = {{Auxin analog-induced Ca 2+ signaling is not involved in inhibition of endosomal aggregation in Arabidopsis roots}},
  url          = {{http://doi.org/10.1093/jxb/erac019}},
  volume       = {{73}},
  year         = {{2022}},
}
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