Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants
- Author
- Simon Strobbe (UGent) , Jana Verstraete, Christophe Stove (UGent) and Dominique Van Der Straeten (UGent)
- Organization
- Abstract
- Thiamin (or thiamine) is a water-soluble B-vitamin (B1), which is required, in the form of thiamin pyrophosphate, as an essential cofactor in crucial carbon metabolism reactions in all forms of life. To ensure adequate metabolic functioning, humans rely on a sufficient dietary supply of thiamin. Increasing thiamin levels in plants via metabolic engineering is a powerful strategy to alleviate vitamin B1 malnutrition and thus improve global human health. These engineering strategies rely on comprehensive knowledge of plant thiamin metabolism and its regulation. Here, multiple metabolic engineering strategies were examined in the model plant Arabidopsis thaliana. This was achieved by constitutive overexpression of the three biosynthesis genes responsible for B1 synthesis, HMP-P synthase (THIC), HET-P synthase (THI1), and HMP-P kinase/ TMP pyrophosphorylase (TH1), either separate or in combination. By monitoring the levels of thiamin, its phosphorylated entities, and its biosynthetic intermediates, we gained insight into the effect of either strategy on thiamin biosynthesis. Moreover, expression analysis of thiamin biosynthesis genes showed the plant's intriguing ability to respond to alterations in the pathway. Overall, we revealed the necessity to balance the pyrimidine and thiazole branches of thiamin biosynthesis and assessed its biosynthetic intermediates. Furthermore, the accumulation of nonphosphorylated intermediates demonstrated the inefficiency of endogenous thiamin salvage mechanisms. These results serve as guidelines in the development of novel thiamin metabolic engineering strategies.
- Keywords
- ARABIDOPSIS-THALIANA, GENE-EXPRESSION, PROTEIN, IDENTIFICATION, RICE, THI1, PYROPHOSPHOKINASE, BIOFORTIFICATION, TRIPHOSPHATE, DEFICIENCY
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8734332
- MLA
- Strobbe, Simon, et al. “Metabolic Engineering Provides Insight into the Regulation of Thiamin Biosynthesis in Plants.” PLANT PHYSIOLOGY, vol. 186, no. 4, 2021, pp. 1832–47, doi:10.1093/plphys/kiab198.
- APA
- Strobbe, S., Verstraete, J., Stove, C., & Van Der Straeten, D. (2021). Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants. PLANT PHYSIOLOGY, 186(4), 1832–1847. https://doi.org/10.1093/plphys/kiab198
- Chicago author-date
- Strobbe, Simon, Jana Verstraete, Christophe Stove, and Dominique Van Der Straeten. 2021. “Metabolic Engineering Provides Insight into the Regulation of Thiamin Biosynthesis in Plants.” PLANT PHYSIOLOGY 186 (4): 1832–47. https://doi.org/10.1093/plphys/kiab198.
- Chicago author-date (all authors)
- Strobbe, Simon, Jana Verstraete, Christophe Stove, and Dominique Van Der Straeten. 2021. “Metabolic Engineering Provides Insight into the Regulation of Thiamin Biosynthesis in Plants.” PLANT PHYSIOLOGY 186 (4): 1832–1847. doi:10.1093/plphys/kiab198.
- Vancouver
- 1.Strobbe S, Verstraete J, Stove C, Van Der Straeten D. Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants. PLANT PHYSIOLOGY. 2021;186(4):1832–47.
- IEEE
- [1]S. Strobbe, J. Verstraete, C. Stove, and D. Van Der Straeten, “Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants,” PLANT PHYSIOLOGY, vol. 186, no. 4, pp. 1832–1847, 2021.
@article{8734332,
abstract = {{Thiamin (or thiamine) is a water-soluble B-vitamin (B1), which is required, in the form of thiamin pyrophosphate, as an essential cofactor in crucial carbon metabolism reactions in all forms of life. To ensure adequate metabolic functioning, humans rely on a sufficient dietary supply of thiamin. Increasing thiamin levels in plants via metabolic engineering is a powerful strategy to alleviate vitamin B1 malnutrition and thus improve global human health. These engineering strategies rely on comprehensive knowledge of plant thiamin metabolism and its regulation. Here, multiple metabolic engineering strategies were examined in the model plant Arabidopsis thaliana. This was achieved by constitutive overexpression of the three biosynthesis genes responsible for B1 synthesis, HMP-P synthase (THIC), HET-P synthase (THI1), and HMP-P kinase/ TMP pyrophosphorylase (TH1), either separate or in combination. By monitoring the levels of thiamin, its phosphorylated entities, and its biosynthetic intermediates, we gained insight into the effect of either strategy on thiamin biosynthesis. Moreover, expression analysis of thiamin biosynthesis genes showed the plant's intriguing ability to respond to alterations in the pathway. Overall, we revealed the necessity to balance the pyrimidine and thiazole branches of thiamin biosynthesis and assessed its biosynthetic intermediates. Furthermore, the accumulation of nonphosphorylated intermediates demonstrated the inefficiency of endogenous thiamin salvage mechanisms. These results serve as guidelines in the development of novel thiamin metabolic engineering strategies.}},
author = {{Strobbe, Simon and Verstraete, Jana and Stove, Christophe and Van Der Straeten, Dominique}},
issn = {{0032-0889}},
journal = {{PLANT PHYSIOLOGY}},
keywords = {{ARABIDOPSIS-THALIANA,GENE-EXPRESSION,PROTEIN,IDENTIFICATION,RICE,THI1,PYROPHOSPHOKINASE,BIOFORTIFICATION,TRIPHOSPHATE,DEFICIENCY}},
language = {{eng}},
number = {{4}},
pages = {{1832--1847}},
title = {{Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants}},
url = {{http://dx.doi.org/10.1093/plphys/kiab198}},
volume = {{186}},
year = {{2021}},
}
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