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A bioanalytical screening method for Enterococcus faecalis RNPP-type quorum sensing peptides in murine feces

Frederick Verbeke (UGent) , Nathan Debunne (UGent) , Yorick Janssens (UGent) , Bart De Spiegeleer (UGent) and Evelien Wynendaele (UGent)
(2022) BIOANALYSIS. 14(3). p.151-167
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Abstract
Background: Bacteria coordinate their behavior as a group via communication with their peers, known as 'quorum sensing'. Enterococcus faecalis employs quorum sensing via RNPP-peptides which were not yet reported to be present in mammalian biofluids. Results: Solid phase extraction of murine feces was performed, followed by ultra high performance liquid chromatography (UHPLC-MS/MS) in multiple reaction monitoring (MRM) mode (in total <90 min/sample) for the nine known RNPP peptides. Limits of detection ranged between 0.045 and 52 nM. Adequate identification criteria allowed detection of RNPP quorum sensing peptides in 2/20 wild-type murine feces samples (i.e., cAM373 and cOB1). Conclusion: A fit-for-purpose UHPLC-MS/MS method detected these RNPP peptides in wild-type murine feces samples.
Keywords
Medical Laboratory Technology, Clinical Biochemistry, General Pharmacology, Toxicology and Pharmaceutics, General Medicine, Analytical Chemistry, Enterococcus faecalis, mass spectrometry, mice feces, microbiome, quorum sensing peptides, UHPLC, SEX-PHEROMONE INHIBITOR, TETRACYCLINE RESISTANCE PLASMID, FECAL MICROBIOTA, BACTERIA, QUANTIFICATION, COMMUNICATION, INFECTIONS, CANCER

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MLA
Verbeke, Frederick, et al. “A Bioanalytical Screening Method for Enterococcus Faecalis RNPP-Type Quorum Sensing Peptides in Murine Feces.” BIOANALYSIS, vol. 14, no. 3, 2022, pp. 151–67, doi:10.4155/bio-2021-0225.
APA
Verbeke, F., Debunne, N., Janssens, Y., De Spiegeleer, B., & Wynendaele, E. (2022). A bioanalytical screening method for Enterococcus faecalis RNPP-type quorum sensing peptides in murine feces. BIOANALYSIS, 14(3), 151–167. https://doi.org/10.4155/bio-2021-0225
Chicago author-date
Verbeke, Frederick, Nathan Debunne, Yorick Janssens, Bart De Spiegeleer, and Evelien Wynendaele. 2022. “A Bioanalytical Screening Method for Enterococcus Faecalis RNPP-Type Quorum Sensing Peptides in Murine Feces.” BIOANALYSIS 14 (3): 151–67. https://doi.org/10.4155/bio-2021-0225.
Chicago author-date (all authors)
Verbeke, Frederick, Nathan Debunne, Yorick Janssens, Bart De Spiegeleer, and Evelien Wynendaele. 2022. “A Bioanalytical Screening Method for Enterococcus Faecalis RNPP-Type Quorum Sensing Peptides in Murine Feces.” BIOANALYSIS 14 (3): 151–167. doi:10.4155/bio-2021-0225.
Vancouver
1.
Verbeke F, Debunne N, Janssens Y, De Spiegeleer B, Wynendaele E. A bioanalytical screening method for Enterococcus faecalis RNPP-type quorum sensing peptides in murine feces. BIOANALYSIS. 2022;14(3):151–67.
IEEE
[1]
F. Verbeke, N. Debunne, Y. Janssens, B. De Spiegeleer, and E. Wynendaele, “A bioanalytical screening method for Enterococcus faecalis RNPP-type quorum sensing peptides in murine feces,” BIOANALYSIS, vol. 14, no. 3, pp. 151–167, 2022.
@article{8733668,
  abstract     = {{Background: Bacteria coordinate their behavior as a group via communication with their peers, known as 'quorum sensing'. Enterococcus faecalis employs quorum sensing via RNPP-peptides which were not yet reported to be present in mammalian biofluids. Results: Solid phase extraction of murine feces was performed, followed by ultra high performance liquid chromatography (UHPLC-MS/MS) in multiple reaction monitoring (MRM) mode (in total <90 min/sample) for the nine known RNPP peptides. Limits of detection ranged between 0.045 and 52 nM. Adequate identification criteria allowed detection of RNPP quorum sensing peptides in 2/20 wild-type murine feces samples (i.e., cAM373 and cOB1). Conclusion: A fit-for-purpose UHPLC-MS/MS method detected these RNPP peptides in wild-type murine feces samples.}},
  author       = {{Verbeke, Frederick and Debunne, Nathan and Janssens, Yorick and De Spiegeleer, Bart and Wynendaele, Evelien}},
  issn         = {{1757-6180}},
  journal      = {{BIOANALYSIS}},
  keywords     = {{Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology,Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry,Enterococcus faecalis,mass spectrometry,mice feces,microbiome,quorum sensing peptides,UHPLC,SEX-PHEROMONE INHIBITOR,TETRACYCLINE RESISTANCE PLASMID,FECAL MICROBIOTA,BACTERIA,QUANTIFICATION,COMMUNICATION,INFECTIONS,CANCER}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{151--167}},
  title        = {{A bioanalytical screening method for Enterococcus faecalis RNPP-type quorum sensing peptides in murine feces}},
  url          = {{http://dx.doi.org/10.4155/bio-2021-0225}},
  volume       = {{14}},
  year         = {{2022}},
}

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