A bioanalytical screening method for Enterococcus faecalis RNPP-type quorum sensing peptides in murine feces
- Author
- Frederick Verbeke (UGent) , Nathan Debunne (UGent) , Yorick Janssens (UGent) , Bart De Spiegeleer (UGent) and Evelien Wynendaele (UGent)
- Organization
- Project
- Abstract
- Background: Bacteria coordinate their behavior as a group via communication with their peers, known as 'quorum sensing'. Enterococcus faecalis employs quorum sensing via RNPP-peptides which were not yet reported to be present in mammalian biofluids. Results: Solid phase extraction of murine feces was performed, followed by ultra high performance liquid chromatography (UHPLC-MS/MS) in multiple reaction monitoring (MRM) mode (in total <90 min/sample) for the nine known RNPP peptides. Limits of detection ranged between 0.045 and 52 nM. Adequate identification criteria allowed detection of RNPP quorum sensing peptides in 2/20 wild-type murine feces samples (i.e., cAM373 and cOB1). Conclusion: A fit-for-purpose UHPLC-MS/MS method detected these RNPP peptides in wild-type murine feces samples.
- Keywords
- Medical Laboratory Technology, Clinical Biochemistry, General Pharmacology, Toxicology and Pharmaceutics, General Medicine, Analytical Chemistry, Enterococcus faecalis, mass spectrometry, mice feces, microbiome, quorum sensing peptides, UHPLC, SEX-PHEROMONE INHIBITOR, TETRACYCLINE RESISTANCE PLASMID, FECAL MICROBIOTA, BACTERIA, QUANTIFICATION, COMMUNICATION, INFECTIONS, CANCER
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8733668
- MLA
- Verbeke, Frederick, et al. “A Bioanalytical Screening Method for Enterococcus Faecalis RNPP-Type Quorum Sensing Peptides in Murine Feces.” BIOANALYSIS, vol. 14, no. 3, 2022, pp. 151–67, doi:10.4155/bio-2021-0225.
- APA
- Verbeke, F., Debunne, N., Janssens, Y., De Spiegeleer, B., & Wynendaele, E. (2022). A bioanalytical screening method for Enterococcus faecalis RNPP-type quorum sensing peptides in murine feces. BIOANALYSIS, 14(3), 151–167. https://doi.org/10.4155/bio-2021-0225
- Chicago author-date
- Verbeke, Frederick, Nathan Debunne, Yorick Janssens, Bart De Spiegeleer, and Evelien Wynendaele. 2022. “A Bioanalytical Screening Method for Enterococcus Faecalis RNPP-Type Quorum Sensing Peptides in Murine Feces.” BIOANALYSIS 14 (3): 151–67. https://doi.org/10.4155/bio-2021-0225.
- Chicago author-date (all authors)
- Verbeke, Frederick, Nathan Debunne, Yorick Janssens, Bart De Spiegeleer, and Evelien Wynendaele. 2022. “A Bioanalytical Screening Method for Enterococcus Faecalis RNPP-Type Quorum Sensing Peptides in Murine Feces.” BIOANALYSIS 14 (3): 151–167. doi:10.4155/bio-2021-0225.
- Vancouver
- 1.Verbeke F, Debunne N, Janssens Y, De Spiegeleer B, Wynendaele E. A bioanalytical screening method for Enterococcus faecalis RNPP-type quorum sensing peptides in murine feces. BIOANALYSIS. 2022;14(3):151–67.
- IEEE
- [1]F. Verbeke, N. Debunne, Y. Janssens, B. De Spiegeleer, and E. Wynendaele, “A bioanalytical screening method for Enterococcus faecalis RNPP-type quorum sensing peptides in murine feces,” BIOANALYSIS, vol. 14, no. 3, pp. 151–167, 2022.
@article{8733668,
abstract = {{Background: Bacteria coordinate their behavior as a group via communication with their peers, known as 'quorum sensing'. Enterococcus faecalis employs quorum sensing via RNPP-peptides which were not yet reported to be present in mammalian biofluids. Results: Solid phase extraction of murine feces was performed, followed by ultra high performance liquid chromatography (UHPLC-MS/MS) in multiple reaction monitoring (MRM) mode (in total <90 min/sample) for the nine known RNPP peptides. Limits of detection ranged between 0.045 and 52 nM. Adequate identification criteria allowed detection of RNPP quorum sensing peptides in 2/20 wild-type murine feces samples (i.e., cAM373 and cOB1). Conclusion: A fit-for-purpose UHPLC-MS/MS method detected these RNPP peptides in wild-type murine feces samples.}},
author = {{Verbeke, Frederick and Debunne, Nathan and Janssens, Yorick and De Spiegeleer, Bart and Wynendaele, Evelien}},
issn = {{1757-6180}},
journal = {{BIOANALYSIS}},
keywords = {{Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology,Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry,Enterococcus faecalis,mass spectrometry,mice feces,microbiome,quorum sensing peptides,UHPLC,SEX-PHEROMONE INHIBITOR,TETRACYCLINE RESISTANCE PLASMID,FECAL MICROBIOTA,BACTERIA,QUANTIFICATION,COMMUNICATION,INFECTIONS,CANCER}},
language = {{eng}},
number = {{3}},
pages = {{151--167}},
title = {{A bioanalytical screening method for Enterococcus faecalis RNPP-type quorum sensing peptides in murine feces}},
url = {{http://doi.org/10.4155/bio-2021-0225}},
volume = {{14}},
year = {{2022}},
}
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